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Identification of consistent QTL with large effect on anther extrusion in doubled haploid populations developed from spring wheat accessions in German Federal ex situ Genebank

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Abstract

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Novel large-effect consistent QTL for anther extrusion (AE) to improve cross-pollination were mapped in doubled haploid populations derived from IPK gene bank spring wheat accessions. TaAP2-D, an ortholog of Cleistogamy1 in barley, is a likely candidate gene for AE in wheat.

Abstract

To establish a robust hybrid wheat breeding system, male lines harboring alleles that promote outcrossing should be developed. In this study, we developed two doubled haploid (DH) populations of hexaploid spring wheat (Triticum aestivum L.) by crossing accessions taken from IPK gene bank. In both populations, the phenotypic data of anther extrusion (AE) based on three years of field trials showed a wide variation and approximated a normal distribution. Both populations were genotyped with a 15 k Infinium single nucleotide polymorphism (SNP) array resulting in 3567 and 3457 polymorphic SNP markers for DH population-1 and DH population-2, respectively. Composite interval mapping identified quantitative trait loci (QTL) on chromosomes 1D, 2D, 4A, 4B, 5A, 5D, 6A, and 6B; with consistent QTL (that are identified in all the years) on chromosome 4A in DH population-1, and on chromosomes 2D and 6B in DH population-2. The consistent QTL explained 17.2%, 32.9%, and 12.3% of the phenotypic variances, respectively. Genic scan of the chromosome 2D-QTL showed that the wheat gene TaAP2-D, an ortholog of Cleistogamy1 which promotes AE via swelling of the lodicules in barley, lies within the QTL region. A diagnostic marker was developed for TaAP2-D that showed co-segregation with the AE phenotype. This study shows the use of gene bank diversity reservoir to find alleles which are otherwise difficult to detect in elite populations. The identification of large-effect consistent QTL for AE is expected to help form efficient male parental lines suitable for hybrid wheat seed production and serve as a source for map-based cloning.

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Acknowledgements

This study was funded by Grant4Traits, a project by Bayer AG, Division Crop Science. We are grateful to Ellen Weiß, Anette Heber, Ute Ostermann, and Sonja Allner for help in data collection. We thank Ravi Koppolu and Martin Mascher for helpful discussions. The authors gratefully acknowledge the handling editor and three anonymous reviewers whose comments helped to improve this manuscript.

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  1. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, 06466, Stadt Seeland, OT Gatersleben, Germany

    Quddoos H. Muqaddasi, Murukarthick Jayakodi, Andreas Börner & Marion S. Röder

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  1. Quddoos H. Muqaddasi
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  2. Murukarthick Jayakodi
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Muqaddasi, Q.H., Jayakodi, M., Börner, A. et al. Identification of consistent QTL with large effect on anther extrusion in doubled haploid populations developed from spring wheat accessions in German Federal ex situ Genebank. Theor Appl Genet 132, 3035–3045 (2019). https://doi.org/10.1007/s00122-019-03404-2

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