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Identification and validation of novel low-tiller number QTL in common wheat

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Abstract

Key message

SNP-based QTL mapping provided useful information for novel loci that can be used in breeding programs to control tillering and improve yield in wheat via marker-assisted selection.

Abstract

Tillering is one of the most important agronomic traits affecting biomass and grain yield potential in wheat. Wheat lines with very limited tillering capacity are more productive than free-tillering lines under severe drought conditions. In this study, three recombinant inbred line (RIL) populations were generated and used, having H461, a low-tillering genotype, as a common parent. A linkage map containing 7808 single nucleotide polymorphism loci was constructed on the basis of H461/CN16 RIL population. Three QTL controlling low tillering were identified on Chromosome (Chr.) 2D (Qltn.sicau-2D), Chr. 2B (Qltn.sicau-2B), and Chr. 5A (Qltn.sicau-5A). Qltn.sicau-2D, Qltn.sicau-2B, and Qltn.sicau-5A explained up to 19.1, 14.6, and 9.6 % of the phenotypic variance, respectively. Comparing with previous findings, Qltn.sicau-2D and Qltn.sicau-2B should thus be novel tillering QTL. The effects of these QTL were further validated in two additional RIL populations. Significant effects of Qltn.sicau-2D were detected across all growth stages in different genetic backgrounds, making it an ideal target for breeding programs as well as for further characterization of the gene(s) underlying this locus.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31301317) and the International Science and Technology Cooperation Program of China (No. 2015DFA30600).

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    Authors and Affiliations

    1. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, China

      Zhiqiang Wang, Yaxi Liu, Haoran Shi, Hongjun Mo, Fangkun Wu, Yu Lin, Shang Gao, Jirui Wang, Yuming Wei & Youliang Zheng

    2. CSIRO Agriculture Flagship, 306 Carmody Road, St Lucia, QLD, 4067, Australia

      Chunji Liu

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    1. Zhiqiang Wang
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    Correspondence to Yaxi Liu.

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    Additional information

    Communicated by G. Muehlbauer.

    Z. Wang and H. Shi contributed equally to this work.

    Electronic supplementary material

    Below is the link to the electronic supplementary material.

    Figure S1: The frequency of tiller number in H461/CN16 population (PDF 288 kb)

    122_2015_2652_MOESM2_ESM.pdf

    Figure S2: Linkage maps constructed using a recombination inbred line population derived from H461/CN16 on chromosome A (PDF 728 kb)

    122_2015_2652_MOESM3_ESM.pdf

    Figure S3: Linkage maps constructed using a recombination inbred line population derived from H461/CN16 on chromosome B (PDF 771 kb)

    122_2015_2652_MOESM4_ESM.pdf

    Figure S4: Linkage maps constructed using a recombination inbred line population derived from H461/CN16 on chromosome D (PDF 305 kb)

    Figure S5: The new genetic linkage map with Xgwm539 (PDF 135 kb)

    122_2015_2652_MOESM6_ESM.pdf

    Table S1: Co-located loci not listed on the linkage map and their corresponding chromosome (Chr) and loci on the map (PDF 712 kb)

    Table S2: QTL x environmental interaction by the mixed linear-model approach for the tiller (PDF 183 kb)

    Table S3: Information for HRM markers developed based on the linkage SNP markers (PDF 185 kb)

    Table S4: Effects of Qltn.sicau-2B in two validation populations (PDF 237 kb)

    Table S5: Effects of Qltn.sicau-5A in two validation populations (PDF 237 kb)

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    Wang, Z., Liu, Y., Shi, H. et al. Identification and validation of novel low-tiller number QTL in common wheat. Theor Appl Genet 129, 603–612 (2016). https://doi.org/10.1007/s00122-015-2652-4

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