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The detection of a de novo allele of the Glu-1Dx gene in wheat–rye hybrid offspring

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This study provides a link between a de novo gene and novel phenotype in wheat–rye hybrids that can be used as a model for induced de novo genetic variation.

Abstract

Wide hybridization can produce de novo DNA variation that may cause novel phenotypes. However, there is still a lack of specific links between changed genes and novel phenotypes in wide hybrids. The well-studied high-molecular-weight glutenin subunit (HMW-GS) genes in tribe Triticeae provide a useful model for addressing this issue. In this study, we investigated the feasibility of a wheat–rye hybridization method for inducing de novo phenotypes using the Glu-1Dx2.2 subunit as an example. We developed three hexaploid wheat lines with normal fertility and a Glu-1Dx2.2 variant, named Glu-1Dx2.2 v, derived from three F1 hybrids. The wild-type Glu-1Dx2.2 has two direct repeats of 295 bp length separated by an intervening 101 bp in its central repetitive region. In the mutant Glu-1Dx2.2 v, one copy of the repeats and the intervening sequence were deleted, probably through homology-dependent illegitimate recombination (IR). This study provides a direct link between a de novo allele and novel phenotype. Our results indicate that the wheat–rye method may be a useful tool to induce de novo genetic variations that broaden the genetic diversity for wheat improvement.

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Acknowledgments

We thank the two anonymous reviewers for helpful suggestions. This work was supported by projects of the National Natural Science Foundation of China (31201210, 31271723) and the Scientific Research Foundation of the Education Department of Sichuan Province (11ZB056).

Conflict of interest

All authors read the manuscript and do not have any conflict of interest.

Ethical standard

The experiments reported here comply with the current laws of the countries in which they were performed.

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Authors and Affiliations

  1. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China

    Zhongwei Yuan, Miao Liu, Yuyuan Ouyang, Xiaoxue Zeng, Ming Hao, Lianquan Zhang, Shunzong Ning, Zehong Yan & Dengcai Liu

  2. Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, China

    Dengcai Liu

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  1. Zhongwei Yuan
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  2. Miao Liu
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  3. Yuyuan Ouyang
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  4. Xiaoxue Zeng
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  5. Ming Hao
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  8. Zehong Yan
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  9. Dengcai Liu
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Corresponding author

Correspondence to Dengcai Liu.

Additional information

Communicated by Bernd Friebe.

Z. Yuan, M. Liu and Y. Ouyang have contributed equally to this work.

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Yuan, Z., Liu, M., Ouyang, Y. et al. The detection of a de novo allele of the Glu-1Dx gene in wheat–rye hybrid offspring. Theor Appl Genet 127, 2173–2182 (2014). https://doi.org/10.1007/s00122-014-2370-3

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  • DOI: https://doi.org/10.1007/s00122-014-2370-3

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