Abstract
Four genes encoding novel 1Dx-type high-molecular weight (HMW) subunits were amplified by polymerase chain reaction, two each from Aegilops tauschii and bread wheat Triticum aestivum. The two subunits from Ae. tauschii (1Dx2.1t and 1Dx2t) were both very similar in sequence to subunit 1Dx2 from bread wheat. In contrast, the two novel bread wheat subunits (1Dx2.2 and 1Dx2.2*) differed from subunit 1Dx2 in having different internally duplicated regions (of 132 and 186 amino acid, respectively) within their repetitive domains. These duplicated sequences were located adjacent to the regions from which they had been duplicated and had complete intact repeat motifs at each end. The implications of these results for HMW subunit evolution and wheat quality improvement are discussed.
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Alvarez JB, D’Ovidio R, Lafiandra D (1997) Comparison of allelic x-type genes present at the Glu-D1 locus in bread wheat by PCR and sequence analysis of their N-terminal domain. J Genet Breed 51:61–166
Anderson OD, Greene FC, Yip RE, Halford NG, Shewry PR, Malpica-Romero J-M (1989) Nucleotide sequences of the two high-molecular-weight glutenen genes from the D-genome of a hexaploid bread wheat, Triticum aestivum L. cv Cheyenne. Nucleic Acids Res 17:461–462
Békés F, Anderson OD, Gras PW, Gupta RB, Tam A, Wrigley CW, Appels R (1994) The contribution to mixing properties of 1D HMW glutenin subunits expressed in a bacterial system. In: Henry RJ, Ronalds JA (eds) Improvement of cereal quality by genetic engineering. Plenum Press, New York, pp 97–103
Békés F, Gras PW, Gupta RB (1995) The effect of purified cereal polypeptides on the mixing properties of dough. In: Williams YA, Wrigley CW (eds) Proceedings of the 45th Australian Cereal Chemistry Conference. The Royal Australian Chemical Institute, Cereal Chemistry Division, pp 92–98
Belton PS (1999) On the elasticity of wheat gluten. J Cereal Sci 29:103–107
D’Ovidio R, Porceddu E, Lafiandra D (1994) PCR analysis of genes encoding allelic variants of high-molecular-weight glutenin subunits at the Glu-Di locus. Theor Appl Genet 88:175–180
D’Ovidio R, Lafiandra D, Porceddu E (1996) Identification and molecular characterisation of a large insertion within the repetitive domain of a high-molecular-weight glutenin subunit gene from hexaploid wheat. Theor Appl Genet 93:1048–1053
Feeney KA, Wellner N, Gilbert SM, Halford NG, Tatham AS, Shewry PR, Belton PS (2003) Molecular structures and interactions of repetitive peptides based on wheat glutenin subunits depend on chain length. Biopolym Biospectrosc 72:123–131
Feldman M (2000) Origin of cultivated wheat. In: Bonjean AP, Angus WJ (eds) The world wheat book: a history of wheat breeding. Intercept Ltd, London, pp 3–56
Feldman M, Lupton FGH, Miller TE (1995) Wheats. In: Smartt J, Simmonds NW (eds) Evolution of crop plants. Longman Group Ltd, London, pp 184–192
Gage LP, Manning RF (1980) Internal structure of the silk fibroin gene of Bombyx mori. I. The fibroin gene consists of a homogeneous alternating array of repetitious crystalline and amorphous coding sequences. J Biol Chem 255:9444–9450
Halford NG, Forde J, Anderson OD, Green FC, Shewry PR (1987) The nucleotide and deduced amino acid sequences of an HMW glutenin subunit gene from chromosome 1B of bread wheat (Triticum aestivum L.), and comparison with those of genes from chromosomes 1A and 1D. Theor Appl Genet 75:117–126
Lagudah ES, Halloran GM (1988) Phylogenetic relationships of Triticum tauschii the D genome donor to hexaploid wheat. I. Variation in HMW subunits of glutenin and gliadins. Theor Appl Genet 75:592–598
Lewin B (1997) Genes VI. Oxford University Press, Oxford
Lewis R (2003) Sequences, structures and properties of spider silks. In: Shewry PR, Tatham AS, Bailey AJ (eds) Elastomeric proteins: structures, biomechanical properties and biological roles. Cambridge University Press, Cambridge, pp 136–151
Mackie AM, Lagudah ES, Sharp PJ, Lafiandra D (1996) Molecular and biochemical characterisation of HMW glutenin subunits from T. tauschii and the D genome of hexaploid wheat. J Cereal Sci 23:213–225
Manning RF, Gage LP (1980) Internal structure of the silk fibroin gene of Bombyx mori. II. Remarkable polymorphism of the organisation of the crystalline and amorphous coding sequences. J Biol Chem 255:9451–9457
Margiotta B, Colaprico G, D’Ovidio R, Lafiandra D (1993) Characterisation of high M r subunits of glutenin by combined chromatographis (RP-HPLC) and electrophoretic separations and restriction fragment length polymorphism (RFLP) analyses of their encoding genes. J Cereal Sci 17:221–236
Massa AN, Morris CF, Gill BS (2004) Sequence diversity of puroindoline-a, puroindoline-b and the grain softness protein genes in Aegilops tauschii Coss. Crop Sci 44:1808–1816
Morris CF (2002) Puroindolines: the molecular genetic basis of wheat grain hardness. Plant Mol Biol 48:633–647
Nakamura H, Hirano H, Sasaki H, Yamashita A (1990) A high molecular weight subunit of wheat glutenin seed storage protein correlates with its flour quality. Jpn J Breed 40:485–494
Payne PI (1987) Genetics of wheat storage proteins and the effect of allelic variation on break making quality. Annu Rev Plant Physiol 38:141–153
Payne PI, Holt LM, Lawrence GD (1983) Detection of a novel high-molecular-weight subunit of glutenin in some Japanese hexaploid wheats. J Cereal Sci 1:3–8
Popineau Y, Cornec M, Lefebvre J, Marchylo B (1994) Influence of high M r glutenin subunits on glutenin polymers and rheological properties of gluten and gluten subfractions of near-isogenic lines of wheat Sicco. J Cereal Sci 19:231–241
Shewry PR, Halford NG, Tatham AS (1989) The high molecular weight subunits of wheat, barley and rye: genetics, molecular biology, chemistry and role in wheat gluten structure and functionality. In: Miflin BJ (ed) Oxford surveys of plant molecular and cell biology, vol 6. Oxford University Press, Oxford, pp 163–219
Shewry PR, Tatham AS, Fido RJ (1995) Separation of plant proteins by electrophoresis. In: Jones H (ed) Methods in molecular biology—plant gene transfer and expression protocols, vol 49. Humana Press, USA, pp 399–422
Shewry PR, Halford NG, Lafiandra D (2003a) In: Hall JC, Dunlap JC, Friedman T (eds) The genetics of wheat gluten proteins. Adv Genet, vol 49. Academic Press, San Diego, pp 111–184
Shewry PR, Halford NG, Tatham AS, Popineau Y, Lafiandra D, Belton PS (2003b) The high molecular weight subunits of wheat glutenin and their role in determining wheat processing properties. Adv Food Nutr Res 45:221–302
Shewry PR, Tatham AS, Bailey A (eds) (2003c) Elastomeric proteins. Cambridge University Press, Cambridge, pp 331–351
Sugiyama T, Rafalski A, Peterson D, Sol D (1985) A wheat HMW glutenin subunit gene reveals a highly repeated structure. Nucleic Acids Res 13:8729–8737
Tatham AS, Shewry PR (2003) Comparative structures and properties of elastic proteins. In: Shewry PR, Tatham AS, Bailey AJ (eds) Elastomeric proteins: structures, biomechanical properties and biological roles. Cambridge University Press, Cambridge, pp 338–351
Wan Y, Liu K, Wang D, Shewry PR (2000) High-molecular-weight glutenin subunits in the Cylindropyrum and Vertebrata section of the Aegilops genus and identification of subunits related to those encoded by the Dx alleles of common wheat. Theor Appl Genet 101:879–884
Wan Y, Wang D, Shewry PR, Halford NG (2002) Isolation and characterisation of five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Aegilops cylindrica. Theor Appl Genet 104:828–839
William MDHM, Peňa RJ, Mujeeb-Kazi A (1993) Seed protein and isozyme variations in Triticum tauschii (Aegilops squarrosa). Theor Appl Genet 87:257–263
Xia L, Chen F, He Z, Chen X, Morris CF (2005) Occurrence of puroindoline alleles in Chinese winter wheats. Cereal Chem 82:38–43
Acknowledgements
Rothamsted Research receives grant-aided support from the Biotechnology and Biological Sciences Research Council of the United Kingdom. Yongfang Wan was supported by a Royal Society China-UK Joint Project Grant (Q813 “Aegilops species as a source of novel genes for wheat improvement”). Daowen Wang was supported by grants (2002CB111301, 2003AA222040) from the Ministry of Science and Technology of China.
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Wan, Y., Yan, Z., Liu, K. et al. Comparative analysis of the D genome-encoded high-molecular weight subunits of glutenin. Theor Appl Genet 111, 1183–1190 (2005). https://doi.org/10.1007/s00122-005-0051-y
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DOI: https://doi.org/10.1007/s00122-005-0051-y