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Meiotic chromosome pairing behaviour of natural tetraploids and induced autotetraploids of Actinidia chinensis

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Abstract

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Non-preferential chromosome pairing was identified in tetraploid Actinidia chinensis and a higher mean multivalent frequency in pollen mother cells was found in colchine-induced tetraploids of A. chinensis compared with naturally occurring tetraploids.

Abstract

Diploid and tetraploid Actinidia chinensis are used for the development of kiwifruit cultivars. Diploid germplasm can be exploited in a tetraploid breeding programme via unreduced (2n) gametes and chemical-induced chromosome doubling of diploid cultivars and selections. Meiotic chromosome behaviour in diploid A. chinensis ‘Hort16A’ and colchicine-induced tetraploids from ‘Hort16A’ was analysed and compared with that in a diploid male and tetraploid males of A. chinensis raised from seeds sourced from the wild in China. Both naturally occurring and induced tetraploids formed multivalents, but colchicine-induced tetraploids showed a higher mean multivalent frequency in the pollen mother cells. Lagging chromosomes at anaphase I and II were observed at low frequencies in the colchicine-induced tetraploids. To investigate whether preferential or non-preferential chromosome pairing occurs in tetraploid A. chinensis, the inheritance of microsatellite alleles was analysed in the tetraploid progeny of crosses between A. chinensis (4x) and A. arguta (4x). The frequencies of inherited microsatellite allelic combinations in the hybrids suggested that non-preferential chromosome pairing had occurred in the tetraploid A. chinensis parent.

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Acknowledgments

We thank A. R. Ferguson, A. G. Seal, H. N. de Silva, and F. A. Gunson for helpful comments on the manuscript, and D. Gibson for photographic design.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

The authors declare that the experiments complied with current laws of the country in which they were performed.

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Authors and Affiliations

  1. The New Zealand Institute for Plant and Food Research Ltd, Auckland Mail Centre, Private Bag 92169, Auckland, 1142, New Zealand

    Jin-Hu Wu, Paul M. Datson & Kelvina I. Manako

  2. School of Biological Sciences, The University of Auckland, Auckland Mail Centre, Private Bag 92019, Auckland, 1142, New Zealand

    Jin-Hu Wu & Brian G. Murray

Authors
  1. Jin-Hu Wu
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  2. Paul M. Datson
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  3. Kelvina I. Manako
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  4. Brian G. Murray
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Corresponding authors

Correspondence to Jin-Hu Wu or Paul M. Datson.

Additional information

Communicated by B. Friebe.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 30 kb)

Supplementary material 2 (DOCX 76 kb)

Supplementary Table 1 Microsatellite primers used in the Actinidia study.

Supplementary Table 2 Multinomial likelihood tests and Pearson’s Chi-squared tests of goodness of fit of observed microsatellite allelic combinations in the hybrid progeny of Actinidia chinensis (4x) x A. arguta (4x) to expected non-preferential pairing, and preferential pairing segregation ratios.

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Wu, JH., Datson, P.M., Manako, K.I. et al. Meiotic chromosome pairing behaviour of natural tetraploids and induced autotetraploids of Actinidia chinensis . Theor Appl Genet 127, 549–557 (2014). https://doi.org/10.1007/s00122-013-2238-y

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  • DOI: https://doi.org/10.1007/s00122-013-2238-y

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