Abstract
Tetraploid plants were induced by colchicine treatment of in vitro leaf petiole segment cultures of five diploid Actinidia chinensis Planch. genotypes, including the commercially important, yellow-fleshed cultivar ‘Hort16A’, three female selections with red-fleshed fruit and one male pollinizer. Petiole segments were incubated on a shoot regeneration medium for a period of 4 weeks, and subsequently microshoots were treated with 0.05 or 0.1% colchicine. About one-third of the regenerated shoots were tetraploid following 0.05% colchicine treatment, more than with 0.1% colchicine treatment. Similar rates of tetraploid induction were achieved with all the genotypes tested. The efficiency of induction of polyploidy depended on the interaction between the types of in vitro culture chosen and the concentration of colchicine used. There are no previous reports of colchicine being used so successfully to induce polyploidy in Actinidia.
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Acknowledgments
We thank CH Cheng, RG Lowe, MA McNeilage and AG Seal for plant material, PA Mooney for helpful discussions, H Ding, AM Duffy, T Machin and P Sutton for technical assistance, D Gibson and JL Zhang for figure design, PM Datson, FA Gunson, D Morgan and JL Yao for critical reading of the manuscript.
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Wu, JH., Ferguson, A.R. & Murray, B.G. Manipulation of ploidy for kiwifruit breeding: in vitro chromosome doubling in diploid Actinidia chinensis Planch.. Plant Cell Tiss Organ Cult 106, 503–511 (2011). https://doi.org/10.1007/s11240-011-9949-z
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DOI: https://doi.org/10.1007/s11240-011-9949-z