Skip to main content
SpringerLink
Log in

Tetrasomic inheritance pattern of the pentaploid Solanum chacoense (+) S. tuberosum somatic hybrid (resistant to bacterial wilt) revealed by SSR detected alleles

  • Original Article
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Abstract

Protoplast fusion may be used to introgress desirable traits from wild species into cultivated potato which are cross incompatible. However, none of commercial varieties have been developed from hyper-tetraploid somatic hybrids because of the unpredictable heredity associated with irregular chromosome segregation during meiosis. A pentaploid somatic hybrid (SH) from Solanum chacoense + S. tuberosum, 3C28-1, which carries bacterial wilt (BW) resistance from the wild species, was employed in the present research. Gamete genotypes and inheritance pattern of SH 3C28-1 were estimated by counting the chromosome numbers and detecting the parent-specific SSR alleles in a backcross population of SH 3C28-1 × E-Potato 1 (variety) which is BW susceptible. The SSR alleles retained from S. chacoense were analyzed for association with BW resistance and evaluation of wild species genetic background. Our results implied that SH 3C28-1 could produce viable gametes of n = 2x = 24 + a where a ≤ 6, which are compatible with a tetraploid pollen source to yield a high proportion of tetraploids and aneuploids with chromosome numbers between 48 and 54. Sixty-four of 83 SH 3C28-1-specific SSR alleles fit the tetrasomic or disomic/tetrasomic segregation ratio of a tetraploid, suggesting that the pentaploid mainly exhibits tetraploid inheritance pattern. Four (three positive and one negative) alleles were estimated to associate with BW resistance and backcross clones were selected for preserving less wild species genetic background and harboring the resistance. The present research elucidates the inheritance pattern of the pentaploid somatic hybrid to provide a theoretical and applied foundation for use of the pentaploid in potato breeding.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Ahn YK, Park TH (2013) Resistance to common scab developed by somatic hybrids between Solanum brevidens and Solanum tuberosum. Acta Agric Scand Sect B Soil Plant Sci 63:595–603

    CAS  Google Scholar 

  • Barone A, Sebastiano A, Carputo D, della Rocca F, Frusciante L (2001) Molecular marker-assisted introgression of the wild Solanum commersonii genome into the cultivated S. tuberosum gene pool. Theor Appl Genet 102:900–907

    Article  CAS  Google Scholar 

  • Barone A, Li J, Sebastiano A, Cardi T, Frusciante L (2002) Evidence for tetrasomic inheritance in a tetraploid Solanum commersonii (+) S. tuberosum somatic hybrid through the use of molecular markers. Theor Appl Genet 104:539–546

    Article  CAS  PubMed  Google Scholar 

  • Butenko R, Kuchko A (1979) Somatic hybridization of Solanum tuberosum L. and Solanum chacoense Bitt. by protoplast fusion. In: Ferenczy L, Farkas GL (eds) Advances in protoplast research. Akademiai Kiado, Budapest, pp 293–300

    Google Scholar 

  • Butenko R, Kuchko A, Komarnitsky I (1982) Some features of somatic hybrids between Solanum tuberosum and Solanum chacoense and its F1 sexual progeny. In: Fujiwara A (ed) Plant tissue culture. Japanese Association for Plant Tissue Culture, Tokyo, pp 643–644

    Google Scholar 

  • Cai X, Liu J, Xie C (2004) Mesophyll protoplast fusion of Solanum tuberosum and Solanum chacoense and their somatic hybrids analysis. Acta Hortic Sin 31:623–626

    Google Scholar 

  • Carputo D (2003) Cytological and breeding behavior of pentaploids derived from 3x × 4x crosses in potato. Theor Appl Genet 106:883–888

    CAS  PubMed  Google Scholar 

  • Carputo D, Parisi M, Consiglio F, Iovene M, Caruso G, Monti L, Frusciante L (2003) Aneuploid hybrids from 5x–4x crosses in potato: chromosome number, fertility, morphology and yield. Am J Potato Res 80:93–101

    Article  Google Scholar 

  • Chen L, Guo X, Xie C, He L, Cai X, Tian L, Song B, Liu J (2013) Nuclear and cytoplasmic genome components of Solanum tuberosum + S. chacoense somatic hybrids and three SSR alleles related to bacterial wilt resistance. Theor Appl Genet 126:1861–1872

    Article  CAS  PubMed  Google Scholar 

  • Cheng J, Saunders JA, Sinden SL (1995) Colorado potato beetle resistant somatic hybrid potato plants produced via protoplast electrofusion. In Vitro Cell Dev Biol Plant 31:90–95

    Article  Google Scholar 

  • Dellaporta S, Wood J, Hicks J (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21

    Article  CAS  Google Scholar 

  • Feingold S, Lloyd J, Norero N, Bonierbale M, Lorenzen J (2005) Mapping and characterization of new EST-derived microsatellites for potato (Solanum tuberosum L.). Theor Appl Genet 111:456–466

    Article  CAS  PubMed  Google Scholar 

  • Fock I, Collonnier C, Purwito A, Luisetti J, Souvannavong V, Vedel F, Servaes A, Ambroise A, Kodja H, Ducreux G, Sihachakr D (2000) Resistance to bacterial wilt in somatic hybrids between Solanum tuberosum and Solanum phureja. Plant Sci 160:165–176

    Article  CAS  PubMed  Google Scholar 

  • Fock I, Collonnier C, Purwito A, Luisetti J, Souvannavong V, Vedel F, Servaes A, Ambroise A, Kodja H, Ducreux G (2001) Use of Solanum stenotomum for introduction of resistance to bacterial wilt in somatic hybrids of potato. Plant Physiol Biochem 39:899–908

    Article  CAS  Google Scholar 

  • Gavrilenko T, Thieme R, Heimbach U, Thieme T (2003) Fertile somatic hybrids of Solanum etuberosum (+) dihaploid Solanum tuberosum and their backcrossing progenies: relationships of genome dosage with tuber development and resistance to potato virus Y. Euphytica 131:323–332

    Article  CAS  Google Scholar 

  • Ghislain M, Nunez J, Herrera MR, Pignataro J, Guzman F, Bonierbale M, Spooner DM (2009) Robust and highly informative microsatellite-based genetic identity kit for potato. Mol Breed 23:377–388

    Article  CAS  Google Scholar 

  • Guo X, Xie C, Cai X, Song B, He L, Liu J (2010) Meiotic behavior of pollen mother cells in relation to ploidy level of somatic hybrids between Solanum tuberosum and S. chacoense. Plant Cell Rep 29:1277–1285

    Article  CAS  PubMed  Google Scholar 

  • Hayward AC (1994) Hosts of Pseudomonas solanacearum. In: Hayward AC, Hartman GL (eds) Bacterial wilt: the disease and its causative agent Pseudomonas solanacearum. CAB International, Wallingford, pp 9–23

    Google Scholar 

  • Helgeson J, Pohlman J, Austin S, Haberlach G, Wielgus S, Ronis D, Zambolim L, Tooley P, McGrath J, James R (1998) Somatic hybrids between Solanum bulbocastanum and potato: a new source of resistance to late blight. Theor Appl Genet 96:738–742

    Article  Google Scholar 

  • Hermsen JGT, Bradshaw JE, Mackay GR (1994) Introgression of genes from wild species, including molecular and cellular approaches. In: Bradshaw JE, Mackay GR (eds) Potato genetics. CAB International, Wallingford, pp 515–538

    Google Scholar 

  • Iovene M, Barone A, Frusciante L, Monti L, Carputo D (2004) Selection for aneuploid potato hybrids combining a low wild genome content and resistance traits from Solanum commersonii. Theor Appl Genet 109:1139–1146

    Article  CAS  PubMed  Google Scholar 

  • Jackson SA, Hanneman REJ (1999) Crossability between cultivated and wild tuber- and non-tuber-bearing Solanums. Euphytica 109:51–67

    Article  Google Scholar 

  • Johnston SA, den Nijs TPM, Peloquin SJ, Hanneman REJ (1980) The significance of genic balance to endosperm development in interspecific crosses. Theor Appl Genet 57:5–9

    Article  CAS  PubMed  Google Scholar 

  • Kim-Lee HY, Moon JS, Hong YJ, Kim MS, Cho HM (2005) Bacterial wilt resistance in the progenies of the fusion hybrids between haploid of potato and Solanum commersonii. Am J Potato Res 82:129–137

    Article  Google Scholar 

  • Laferriere LT, Helgeson JP, Allen C (1999) Fertile Solanum tuberosum + S. commersonii somatic hybrids as sources of resistance to bacterial wilt caused by Ralstonia solanacearum. Theor Appl Genet 98:1272–1278

    Article  Google Scholar 

  • Lamm R (1941) Varying cytological behaviour in reciprocal Solanum crosses. Hereditas 27:202–208

    Article  Google Scholar 

  • Li YH, Han ZH, Xu X (2004) Segregation patterns of AFLP markers in F1 hybrids of a cross between tetraploid and diploid species in genus Malus. Plant Breed 123:316–320

    Article  CAS  Google Scholar 

  • Li J, Lindqvist-Kreuze H, Tian Z, Liu J, Song B, Landeo J, Portal L, Gastelo M, Frisancho J, Sanchez L (2012) Conditional QTL underlying resistance to late blight in a diploid potato population. Theor Appl Genet 124:1339–1350

    Article  CAS  PubMed  Google Scholar 

  • Liu J, Xu X, Deng X (2005) Intergeneric somatic hybridization and its application to crop genetic improvement. Plant Cell Tissue Org Cult 82:19–44

    Article  CAS  Google Scholar 

  • Murakami N, Matsunaga H, Senda K, Okuyama Y, Iritani M, Asama K, Mitsui Y, Shimizu K (1995) A new potato variety “Konamuso (=Sakurafubuki)”. Bull Hokkaido Prefect Agric Exp Stn 68:1–16

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Orczyk W, Przetakiewicz J, Nadolska-Orczyk A (2003) Somatic hybrids of Solanum tuberosum—application to genetics and breeding. Plant Cell Tissue Organ Cult 74:1–13

    Article  CAS  Google Scholar 

  • Sarkar D, Tiwari JK, Sharma S, Poonam Sharma S, Gopal J, Singh BP, Luthra SK, Pandey SK, Pattanayak D (2011) Production and characterization of somatic hybrids between Solanum tuberosum L. and S. pinnatisectum Dun. Plant Cell Tissue Organ Cult 107:427–440

    Article  Google Scholar 

  • Thieme R, Rakosy-Tican E, Gavrilenko T, Antonova O, Schubert J, Nachtigall M, Heimbach U, Thieme T (2008) Novel somatic hybrids (Solanum tuberosum L. + Solanum tarnii) and their fertile BC1 progenies express extreme resistance to potato virus Y and late blight. Theor Appl Genet 116:691–700

    Article  CAS  PubMed  Google Scholar 

  • Thieme R, Rakosy-Tican E, Nachtigall M, Antonova O, Gavrilenko T, Heimbach U, Thieme T (2010) Characterization of the multiple resistance traits of somatic hybrids between Solanum cardiophyllum Lindl. and two commercial potato cultivars. Plant Cell Rep 29:1187–1201

    Article  CAS  PubMed  Google Scholar 

  • Tiwari JK, Poonam B, Sarkar D, Pandey SK, Gopal J, Kumar SR (2010) Molecular and morphological characterization of somatic hybrids between Solanum tuberosum L. and S. etuberosum Lindl. Plant Cell Tissue Organ Cult 103:175–187

    Article  CAS  Google Scholar 

  • Toppino L, Mennella G, Rizza F, D’Alessandro A, Sihachakr D, Rotino GL (2008) ISSR and isozyme characterization of and rogenetic dihaploids reveals tetrasomic inheritance in tetraploid somatic hybrids between Solanum melongena and Solanum aethiopicum group Gilo. J Hered 99:304–315

    Article  CAS  PubMed  Google Scholar 

  • Winstead NN, Kelman A (1952) Inoculation techniques for evaluating resistance to Pseudomonas solanacearum. Phytopathology 42:628–634

    Google Scholar 

  • Yamada T, Hosaka K, Kaide N, Nakagawa K, Misoo S, Kamijima O (1998) Cytological and molecular characterization of BC1 progeny from two somatic hybrids between dihaploid Solanum acaule and tetraploid S. tuberosum. Genome 41:743–750

    Article  CAS  Google Scholar 

  • Yu Y, Ye W, He L, Cai X, Liu T, Liu J (2013) Introgression of bacterial wilt resistance from eggplant to potato via protoplast fusion and genome components of the hybrids. Plant Cell Rep 32:1687–1701

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The work was supported by the earmarked fund for Modern Agro-industry Technology Research System (nycytx-15), the special fund for agro-scientific research in the public interest from the Ministry of Agriculture, China (201303007) and the Ministry of Education of China (IRT13065).

Authors’ contributions

LC conducted the SSR marker selection, bacterial wilt assessment and paper drafting; XG constructed the backcrossing population and investigated the agronomic traits; HW and LH conducted the chromosome counting; JZ assisted on genetic analysis; XC reviewed the progress of the experiments; CX advised on data analysis and paper drafting; JL designed and supervised the study. All the authors read and approved the manuscript.

Author information

Author notes

    Authors and Affiliations

    1. National Center for Vegetable Improvement (Central China), Key Laboratory of Horticultural Plant Biology (Huazhong Agricultural University), Ministry of Education, Potato Engineering and Technology Research Center of Hubei Province, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China

      Lin Chen, Xianpu Guo, Haibo Wang, Conghua Xie, Xingkui Cai, Li He, Jun Zhou & Jun Liu

    2. Wuchang University of Technology, Wuhan, 430223, Hubei, People’s Republic of China

      Xianpu Guo

    Authors
    1. Lin Chen
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Xianpu Guo
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Haibo Wang
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Conghua Xie
      View author publications

      You can also search for this author in PubMed Google Scholar

    5. Xingkui Cai
      View author publications

      You can also search for this author in PubMed Google Scholar

    6. Li He
      View author publications

      You can also search for this author in PubMed Google Scholar

    7. Jun Zhou
      View author publications

      You can also search for this author in PubMed Google Scholar

    8. Jun Liu
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding author

    Correspondence to Jun Liu.

    Ethics declarations

    Conflict of interest

    The authors declare that they have no conflict of interest.

    Additional information

    Lin Chen and Xianpu Guo have contributed equally to this work.

    Electronic supplementary material

    Below is the link to the electronic supplementary material.

    Supplementary material 1 (PDF 345 kb)

    Supplementary Fig. 1

    Flowers of the parents: a. The pentaploid somatic hybrid Solanum chacoense (+) S. tuberosum, 3C28-1, b Tetraploid potato cv. E1 and c. Genotype BC1.17 of the progeny (2n = 52)

    Rights and permissions

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Chen, L., Guo, X., Wang, H. et al. Tetrasomic inheritance pattern of the pentaploid Solanum chacoense (+) S. tuberosum somatic hybrid (resistant to bacterial wilt) revealed by SSR detected alleles. Plant Cell Tiss Organ Cult 127, 315–323 (2016). https://doi.org/10.1007/s11240-016-1051-0

    Download citation

    • Received:

    • Accepted:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s11240-016-1051-0

    Keywords

    Search

    Navigation