Skip to main content
SpringerLink
Log in

Strategy for exploiting exotic germplasm using genetic, morphological, and environmental diversity: the Aegilops tauschii Coss. example

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Hexaploid bread wheat evolved from a rare hybridisation, which resulted in a loss of genetic diversity in the wheat D-genome with respect to the ancestral donor, Aegilops tauschii. Novel genetic variation can be introduced into modern wheat by recreating the above hybridisation; however, the information associated with the Ae. tauschii accessions in germplasm collections is limited, making rational selection of accessions into a re-synthesis programme difficult. We describe methodologies to identify novel diversity from Ae. tauschii accessions that combines Bayesian analysis of genotypic data, sub-species diversity and geographic information that summarises variation in climate and habitat at the collection point for each accession. Comparisons were made between diversity discovered amongst a panel of Ae. tauschii accessions, bread wheat varieties and lines from the CIMMYT synthetic hexaploid wheat programme. The selection of Ae. tauschii accessions based on differing approaches had significant effect on diversity within each set. Our results suggest that a strategy that combines several criteria will be most effective in maximising the sampled variation across multiple parameters. The analysis of multiple layers of variation in ex situ Ae. tauschii collections allows for an informed and rational approach to the inclusion of wild relatives into crop breeding programmes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Allen AM, Barker GLA, Berry ST, Coghill JA, Gwilliam R, Kirby S, Robinson P, Brenchley RC, D’Amore R, McKenzie N, Hall A, Bevan M, Hall N, Edwards KJ (2011) Transcript-specific, single-nucleotide polymorphism discovery and linkage analysis in hexaploid bread wheat (Triticum aestivum L.). Plant Biotechnol J 9:1086–1099

    Article  PubMed  CAS  Google Scholar 

  • Allen AM, Barker GLA, Wilkinson P, Burridge A, Winfield M, Coghill J, Uauy C, Griffiths S, Jack P, Berry S, Werner P, Melichar JPE, McDougall J, Gwilliam R, Robinson P, Edwards KJ (2013) Discovery and development of exome-based, co-dominant single nucleotide polymorphism markers in hexaploid wheat (Triticum aestivum L.). Plant Biotechnol J 11(3):279–295. doi:10.1111/pbi.12009

    Google Scholar 

  • Beales J, Turner A, Griffiths S, Snape JW, Laurie DA (2007) A pseudo-response regulator is misexpressed in the photoperiod insensitive Ppd-D1a mutant of wheat (Triticum aestivum L.). Theor Appl Genet 115:721–733

    Article  PubMed  CAS  Google Scholar 

  • Bentley AR, Turner AS, Gosman N, Leigh FJ, Maccaferri M, Dreisigacker S, Greenland A, Laurie DA (2011) Frequency of photoperiod-insensitive Ppd-A1a alleles in tetraploid, hexaploid and synthetic hexaploid wheat germplasm. Plant Breed 130:10–15

    Article  CAS  Google Scholar 

  • Bhullar NK, Street K, Mackay M, Yahiaouia N, Kellera B (2009) Unlocking wheat genetic resources for the molecular identification of previously undescribed functional alleles at the Pm3 resistance locus. Proc Natl Acad Sci USA 106:9519–9524

    Article  PubMed  CAS  Google Scholar 

  • Brevis JC, Morris CF, Manthey F, Dubcovsky J (2010) Effect of the grain protein content locus Gpc-B1 on bread and pasta quality. J Cereal Sci 51:357–365

    Article  CAS  Google Scholar 

  • Campana MG, Hunt HV, Jones H, White J (2010) CorrSieve: software for summarizing and evaluating Structure output. Mol Ecol Resour 11:340–352

    Google Scholar 

  • Dubcovsky J, Dvorak J (2007) Genome plasticity a key factor in the success of polyploid wheat under domestication. Science 316:1862–1866

    Article  PubMed  CAS  Google Scholar 

  • Dudnikov AJ (2011) Chloroplast DNA non-coding sequences variation in Aegilops tauschii Coss.: evolutionary history of the species. Genet Resour Crop Evol 59:683–699

    Article  Google Scholar 

  • Dvorak J, Zhang HK (1992) Reconstruction of the phylogeny of the genus Triticum from variation in repeated nucleotide sequences. Theor Appl Genet 84:419–429

    Article  Google Scholar 

  • Dvorak J, Luo MC, Yang ZL, Zhang HB (1998a) The structure of the Aegilops tauschii genepool and the evolution of hexaploid wheat. Theor Appl Genet 97:657–670

    Article  CAS  Google Scholar 

  • Dvorak J, Luo M-C, Yang Z-L (1998b) Genetic evidence on the origin of Triticum aestivum L. In: Damania AB, Valkoun J, Willcox G, Qualset CO (eds) The origins of agriculture and crop domestication (The Harlan Symposium). ICARDA, Aleppo, pp 235–251

    Google Scholar 

  • Endresen DTF (2010) Predictive association between trait data and ecogeographic data for Nordic Barley Landraces. Crop Sci 50:2418–2430

    Article  Google Scholar 

  • Endresen DTF, Street K, Mackay M, Bari A, De Pauw E (2011) Predictive association between biotic stress traits and ecogeographic data for wheat and barley landraces. Crop Sci 51:2036–2055

    Article  Google Scholar 

  • ESRI (2011) ArcGIS Desktop: Release 10. Environmental Systems Research Institute, Redlands

    Google Scholar 

  • Eujayl I, Sorrells M, Baum M, Wolters P, Powell W (2002) Isolation of EST-derived microsatellite markers for genotyping the A and B genomes of wheat. Theor Appl Genet 104:399–407

    Article  PubMed  CAS  Google Scholar 

  • Feldman M, Lupton FGH, Miller TE (1995) Wheats. In: Smartt J, Simmonds NW (eds) Evolution of Crops. Longman Scientific, London, pp 184–192

    Google Scholar 

  • Feuillet C, Langridge P, Waugh R (2008) Cereal breeding takes a walk on the wild side. Trends Genet 24:24–32

    Article  PubMed  CAS  Google Scholar 

  • Fulton TM, Chunwongse J, Tanksley SD (1995) Microprep protocol for extraction of DNA from tomato and other herbaceous plants. Plant Mol Biol Rep 13:207–209

    Article  CAS  Google Scholar 

  • Giles RJ, Brown TA (2006) GluDy allele variations in Aegilops tauschii and Triticum aestivum: implications for the origins of hexaploid wheats. Theor Appl Genet 112:1563–1572

    Article  PubMed  CAS  Google Scholar 

  • Gupta PK, Balyan HS, Edwards KJ, Isaac P, Korzun V, Röder M, Gautier M-F, Joudrier P, Schlatter AR, Dubcovsky J et al (2002) Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theor Appl Genet 105:413–422

    Article  PubMed  CAS  Google Scholar 

  • Hamblin MT, Warburton ML, Buckler ES (2007) Empirical comparison of simple sequence repeats and single nucleotide polymorphisms in assessment of maize diversity and relatedness. PLoS One 2:e1367. doi:10.1371/journal.pone.0001367

    Article  PubMed  Google Scholar 

  • Heywood V (2011) Introductory and Background Material. In: Hunter D, Heywood V (eds) Crop Wild Relatives: A Manual of in situ Conservation. London and Washington Earthscan in Association with Biodiversity International, pp 3–30. ISBN 9781849711791

  • Hijmans RJ, Jacobs M, Bamberg JB, Spooner DM (2003) Frost tolerance in wild potato species: assessing the predictivity of taxonomic, geographic, and ecological factors. Euphytica 130:47–59

    Article  Google Scholar 

  • Hübner S, Höffken M, Oren E, Haseneyer G, Stein N, Graner A, Schmid K, Fridman E (2009) Strong correlation of wild barley (Hordeum spontaneum) population and precipitation variation. Mol Ecol 18:1523–1536

    Article  PubMed  Google Scholar 

  • IUCN (1974) Uvardy MDF. A classification of the biogeographical provinces of the world. IUCN occasional paper No 18, International Union for Conservation of Nature, Gland, Switzerland

  • Jiang J, Friebe B, Gill BS (1994) Recent advances in alien gene transfer in wheat. Euphytica 73:199–212

    Article  Google Scholar 

  • Jones H, Lister DL, Bower MA, Leigh FJ, Smith LM, Jones MK (2008) Approaches and constraints of using existing landrace and extant plant material to understand agricultural spread in prehistory. Plant Genet Resour 6:98–112

    Article  Google Scholar 

  • Jones H, Civáň P, Cockram J, Leigh FJ, Smith LMJ, Jones MK, Charles MP, Molina-Cano J-L, Powell W, Jones G, Brown TA (2011) Evolutionary history of barley cultivation in Europe revealed by genetic analysis of extant landraces. BMC Evolut Biol 11:320. http://www.biomedcentral.com/1471-2148/11/320

  • Kilian B, Graner A (2012) NGS technologies for analyzing germplasm diversity in genebanks. Briefings Funct Genomics. doi:10.1093/bfgp/elr046

    Google Scholar 

  • Kishii M, Delgado R, Rosas V, Cortes A, Cano S, Sanchez J, Mujeeb-Kazi A (2007) Exploitation of Genetic Resources through Wide Crosses. Proceedings International Symposium on wheat Yield Potential CIMMYT, Mexico

  • Knaggs P, Ambrose MJ, Reader S, Miller TE (2000) Morphological characterization and evaluation of the subdivision of Aegilops tauschii Coss. Wheat Inform Serv 91:15–19

    Google Scholar 

  • Liu K, Muse SV (2005) Powermarker: integrated analysis environment for genetic marker data. Bioinformatics 21:2128–2129

    Article  PubMed  CAS  Google Scholar 

  • Matsuoka Y (2011) Evolution of polyploid triticum wheats under cultivation: the role of domestication, natural hybridization and allopolyploid speciation in their diversification. Plant Cell Physiol 52:750–764

    Article  PubMed  CAS  Google Scholar 

  • Maxted N, Kell SP (2009) Establishment of a global network for the in situ conservation of crop wild relatives: status and needs. FAO Commission on Genetic Resources for Food and Agriculture, Rome

    Google Scholar 

  • Maxted N, White K, Valkoun J, Konopka J, Hargreaves S (2008) Towards a conservation strategy for Aegilops species. Plant Genetic Resour Charact Utilization 6:126–141

    Google Scholar 

  • Mizuno N, Yamasaki M, Matsuoka Y, Kawahara T, Takumi S (2010) Population structure of wild wheat D-genome progenitor Aegilops tauschii Coss: implications for intraspecific lineage diversification and evolution of common wheat. Mol Ecol 19:999–1013

    Article  PubMed  Google Scholar 

  • Moragues M, Comadran J, Waugh R, Milne I, Flavell AJ, Russell JR (2010) Effects of ascertainment bias and marker number on estimations of barley diversity from high-throughput SNP genotype data. Theor Appl Genet 120:1525–1534

    Article  PubMed  CAS  Google Scholar 

  • Mujeeb-Kazi A, Gul A, Ahmad I, Farooq M, Rizwan S, Bux H, Iftikhar S, Asad S, Delgado R (2007) Aegilops tauschii, as a spot blotch (Cochliobolus sativus) resistance source for bread wheat improvement. Pak J Bot 39:1207–1216

    Google Scholar 

  • New M, Lister D, Hulme M, Makin I (2002) A high-resolution data set of surface climate over global land areas. Clim Res 21:1–25

    Article  Google Scholar 

  • Peeters JP, Wilkes HG, Galwey NW (1990) The use of ecogeographical data in the exploitation of variation from gene banks. Theor Appl Genet 80:110–112

    Article  Google Scholar 

  • Pestsova E, Korzun V, Goncharov NP, Hammer K, Ganal MW, Röder MS (2000) Microsatellite analysis of Aegilops tauschii germplasm. Theor Appl Genet 101:100–106

    Article  CAS  Google Scholar 

  • Prada D (2009) Molecular population genetics and agronomic alleles in seed banks: searching for a needle in a haystack? J Exp Bot 60:2541–2552

    Article  PubMed  CAS  Google Scholar 

  • Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959

    PubMed  CAS  Google Scholar 

  • Reynolds M, Trethowan RM (2007) Physiological interventions in breeding for adaptation to abiotic stress. In: Spiertz JHJ, Struik PC, Van Laar HH (eds) Scale and complexity in plant systems research, gene-plant-crop relations. Springer, The Netherlands

    Google Scholar 

  • Reynolds M, Dreccer F, Trethowan R (2007) Drought-adaptive traits derived from wheat wild relatives and landraces. J Exp Bot 58:177–186

    Article  PubMed  CAS  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M-H, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Sakamoto Y, Ishiguro M, Kitagawa G (1986) Akaike information criterion statistics. D. Reidel Publishing Company, Dordrecht

    Google Scholar 

  • Sohail Q, Shehzad T, Kilian A, Eltayeb AE, Tanaka H, Tsujimoto H (2012) Development of diversity array technology (DArT) markers for assessment of population structure and diversity in Aegilops tauschii. Breed Sci 62:38–45. doi:10.1270/jsbbs.62.38

    Article  PubMed  CAS  Google Scholar 

  • Song QJ, Fickus EW, Cregan PB (2002) Characteristics of trinucleotide markers in wheat. Theor Appl Genet 104:286–293

    Article  PubMed  CAS  Google Scholar 

  • Song QJ, Shi JR, Singh S, Fickus EW, Costa JM, Lewis J, Gill BS, Ward R, Cregan PB (2005) Development and mapping of microsatellite (SSR) markers in wheat. Theor Appl Genet 110:550–560

    Article  PubMed  CAS  Google Scholar 

  • Trethowan RM, Mujeeb-Kazi A (2008) Novel germplasm resources for improving environmental stress tolerance of hexaploid wheat. Crop Sci 48:1255–1265

    Article  Google Scholar 

  • Vavilov NI (1957) World resources of cereals, leguminous seed crops and flax and their utilisation in plant breeding. The Academy of Sciences of the USSR. Moscow translated and published by the National Science Foundation, Washington (1960)

    Google Scholar 

  • Wang J, Luo MC, Chen Z, You FM, Wei Y, Zheng Y, Dvorak J (2013) Aegilops tauschii single nucleotide polymorphisms shed light on the origins of wheat D-genome genetic diversity and pinpoint the geographic origin of hexaploid wheat. New Phytol. doi:10.1111/nph.12164

    Google Scholar 

Download references

Acknowledgments

The authors gratefully acknowledge the NIAB Trust and the UK Biotechnology and Biological Sciences Research Council (grants BB/E006868/1, BB/I002561/1, BB/J004596/1, BB/J004588/1) for funding this work. The following germplasm collections provided seed and passport data for the Ae. tauschii accessions, USDA-ARS Small Grains Collection, University of Tokyo, IPK Gatersleben, ICARDA, Kansas State University, John Innes Centre Germplasm Resources Unit and the Vavilov Institute. The authors wish to thank Jon Raupp of the Kansas State University, Steve Reader and Mike Ambrose of the John Innes Centre.

Author information

Authors and Affiliations

  1. NIAB, Huntingdon Road, Cambridge, CB1 0LE, UK

    H. Jones, N. Gosman, R. Horsnell, G. A. Rose, L. A. Everest, A. R. Bentley, C. Uauy & A. Greenland

  2. John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK

    S. Tha, C. Uauy & A. Kowalski

  3. Agricultural Institute of Osijek, Južno Predgrađe 17, Osijek, Croatia

    D. Novoselovic & R. Simek

  4. Institute of Field and Vegetable Crops, M/Gorkog 30, Novi Sad, Serbia

    B. Kobiljski, A. Kondic-Spika & L. Brbaklic

  5. Vavilov Institute of Plant Industry, 42-44, B.Morskaya Street, 190000, St. Petersburg, Russia

    O. Mitrofanova & Y. Chesnokov

  6. CIMMYT, Km. 45, Carretera Mexico-Veracruz, El Batan, CP 56130, Texcoco, Edo. de México, Mexico

    D. Bonnett

Authors
  1. H. Jones
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. Gosman
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Horsnell
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. A. Rose
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. A. Everest
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. R. Bentley
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. S. Tha
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. C. Uauy
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. A. Kowalski
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. D. Novoselovic
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. R. Simek
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. B. Kobiljski
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. A. Kondic-Spika
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. L. Brbaklic
    View author publications

    You can also search for this author in PubMed Google Scholar

  15. O. Mitrofanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  16. Y. Chesnokov
    View author publications

    You can also search for this author in PubMed Google Scholar

  17. D. Bonnett
    View author publications

    You can also search for this author in PubMed Google Scholar

  18. A. Greenland
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to H. Jones or N. Gosman.

Additional information

Communicated by J. Dubcovsky.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PPTX 3008 kb)

Supplementary material 2 (XLSX 595 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jones, H., Gosman, N., Horsnell, R. et al. Strategy for exploiting exotic germplasm using genetic, morphological, and environmental diversity: the Aegilops tauschii Coss. example. Theor Appl Genet 126, 1793–1808 (2013). https://doi.org/10.1007/s00122-013-2093-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-013-2093-x

Keywords

Search

Navigation