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Positional cloning of a candidate gene for resistance to the sunflower downy mildew, Plasmopara halstedii race 300

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Abstract

The resistance of sunflower to Plasmopara halstedii is conferred by major resistance genes denoted Pl. Previous genetic studies indicated that the majority of these genes are clustered on linkage groups 8 and 13. The Pl6 locus is one of the main clusters to have been identified, and confers resistance to several P. halstedii races. In this study, a map-based cloning strategy was implemented using a large segregating F2 population to establish a fine physical map of this cluster. A marker derived from a bacterial artificial chromosome (BAC) clone was found to be very tightly linked to the gene conferring resistance to race 300, and the corresponding BAC clone was sequenced and annotated. It contains several putative genes including three toll-interleukin receptor-nucleotide binding site-leucine rich repeats (TIR-NBS-LRR) genes. However, only one TIR-NBS-LRR appeared to be expressed, and thus constitutes a candidate gene for resistance to P. halstedii race 300.

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Authors and Affiliations

  1. Clermont Université, Université Blaise Pascal, UMR INRA-UBP 1095 GDEC, BP 10448, 63000, Clermont-Ferrand, France

    Jérôme Franchel, Mohamed Fouad Bouzidi, Felicity Vear, Paul Nicolas & Said Mouzeyar

  2. Clermont Université, Université Blaise Pascal, UMR 547 PIAF, BP 10448, 63000, Clermont-Ferrand, France

    Jérôme Franchel

  3. INRA, UMR 547 PIAF, 63100, Clermont-Ferrand, France

    Jérôme Franchel

  4. Clermont Université, Université Blaise Pascal, Laboratoire “Microorganismes: Génome et Environnement”, BP 10448, 63000, Clermont-Ferrand, France

    Gisèle Bronner

  5. CNRS, UMR 6023, LMGE, 63171, Aubiere, France

    Gisèle Bronner

Authors
  1. Jérôme Franchel
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  2. Mohamed Fouad Bouzidi
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  3. Gisèle Bronner
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  4. Felicity Vear
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  5. Paul Nicolas
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  6. Said Mouzeyar
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Corresponding author

Correspondence to Said Mouzeyar.

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Communicated by A. Bervillé.

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Franchel, J., Bouzidi, M.F., Bronner, G. et al. Positional cloning of a candidate gene for resistance to the sunflower downy mildew, Plasmopara halstedii race 300. Theor Appl Genet 126, 359–367 (2013). https://doi.org/10.1007/s00122-012-1984-6

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  • DOI: https://doi.org/10.1007/s00122-012-1984-6

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