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Fine mapping and candidate gene analysis of the nuclear restorer gene Rfp for pol CMS in rapeseed (Brassica napus L.)

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Abstract

The Polima (pol) system of cytoplasmic male sterility (CMS) in rapeseed is widely used in China for commercial hybrid seed production. Genetic studies have shown that its fertility restorer gene (Rfp) is monogenic dominant. For fine mapping of the Rfp gene, a near isogenic line comprising 3,662 individuals of BC14F1 generation segregating for the Rfp gene was created. Based on the sequences of two SCAR markers, SCAP0612ST and SCAP0612EM2, developed by Zhao et al. (Genes Genom 30(3):191–196, 2008) and the synteny region of Brassica napus and other Brassica species, 13 markers strongly linked with the Rfp gene were identified. By integrating three of these markers to the published linkage map, the Rfp gene was mapped on linkage group N9 of B. napus. Using these markers, the Rfp locus was narrowed down to a 29.2-kb genomic region of Brassica rapa. Seven open reading frames (ORFs) were predicted in the target region, of these, ORF2, encoding a PPR protein, was the most likely candidate gene of Rfp. These results lay a solid foundation for map-based cloning of the Rfp gene and will be helpful for marker-assisted selection of elite CMS restorer lines.

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Abbreviations

BC:

Backcross

CMS:

Cytoplasmic male sterility

NIL:

Near isogenic line

ORF:

Open reading frame

MAS:

Marker-assisted selection

DH:

Doubled haploid

PPR:

Pentatricopeptide repeat

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Acknowledgments

We thank Dr. Xiaowu Wang (Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences) for kindly providing the Scaffold sequence of B. rapa and B. oleracea. We appreciate Dr. William H. White for his critical reading of the revised manuscipt. This research was financially supported by the National Key Basic Research Program (2007CB109005), the Programme for Changjiang Scholar and Innovative Research Team in university (IRT0442) and the National Natural Science Foundation of China (No. 30700449).

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Authors and Affiliations

  1. National Key Lab of Crop Genetic Improvement, National Center of Rapeseed Improvement (Wuhan Branch), Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Zhi Liu, Pingwu Liu, Furong Long, Dengfeng Hong, Qingbiao He & Guangsheng Yang

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  1. Zhi Liu
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  2. Pingwu Liu
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  3. Furong Long
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  4. Dengfeng Hong
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  5. Qingbiao He
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  6. Guangsheng Yang
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Corresponding author

Correspondence to Guangsheng Yang.

Additional information

Communicated by H. Becker.

P. Liu contributed equally to this work.

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Liu, Z., Liu, P., Long, F. et al. Fine mapping and candidate gene analysis of the nuclear restorer gene Rfp for pol CMS in rapeseed (Brassica napus L.). Theor Appl Genet 125, 773–779 (2012). https://doi.org/10.1007/s00122-012-1870-2

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  • DOI: https://doi.org/10.1007/s00122-012-1870-2

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