Abstract
The two forms of cytoplasmic male sterility (CMS) native to the oilseed rape or canola species Brassica napus, nap and pol, have novel features that may provide insight into the molecular mechanisms through which CMS/nuclear restorer systems evolve. One such feature is the finding that the distinct nuclear restorer genes for the two systems represent different alleles or haplotypes of the same nuclear locus. Improved understanding of how these systems have evolved will require molecular cloning and characterization of this novel locus. We have employed an approach that exploits the regional co-linearity between the Arabidopsis and Brassica genomes to construct a high-resolution genetic map of the nuclear restorer for the pol system, Rfp. Specifically, Arabidopsis-derived sequences have been used as a set of ordered RFLP probes to localize Rfp to a region of the B. napus genome equivalent to a 115 kb interval on Arabidopsis chromosome 1. Based on the known relationship of physical distances between orthologous segments of Arabidopsis and Brassica chromosomes, it is anticipated that the B. napus restorer locus is now mapped to sufficient resolution to permit its isolation and characterization.
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Acknowledgments
This work was supported by funds from Discovery and Collaborative Research and Development Grants from the Natural Sciences and Engineering Research Council (NSERC), Canada, by BASF Plant Science and by the Industrial Research Assistance Program of the National Research Council (NRC), Canada. We express thanks to Dr. Rod Wing, University of Arizona, for providing the Arabidopsis BACs and to Dr. Tom Bureau, Department of Biology, McGill University, for analysis of an Arabidopsis genome region for transposable element sequences.
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Formanová, N., Stollar, R., Geddy, R. et al. High-resolution mapping of the Brassica napus Rfp restorer locus using Arabidopsis-derived molecular markers. Theor Appl Genet 120, 843–851 (2010). https://doi.org/10.1007/s00122-009-1215-y
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DOI: https://doi.org/10.1007/s00122-009-1215-y