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Quantitative trait loci for root morphology in response to low phosphorus stress in Brassica napus

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Abstract

Phosphorus (P) deficiency in soils is a major limiting factor for crop growth worldwide. Changes in root morphology and architecture represent as an important mechanism of adaptation of plants to low P (LP) stress. To elucidate the genetic control of tolerance to P deficiency in Brassica napus, quantitative trait loci (QTL) for root morphology in response to LP were identified in three independent paper culture experiments, and dissected through QTL meta-analysis. In total, 62 significant QTL for total root length, root surface area, root volume, total dry weight, and plant P uptake under high and low P conditions were detected in the three experiments. Forty-five of these QTL were clustered within four linkage groups and were integrated into eight unique QTL by two rounds of QTL meta-analysis. Three of the unique QTL, uq.A1, uq.C3a and uq.C3b, were specific for LP condition. uq.C3a and uq.C3b were identified specifically for root traits and P uptake under LP stress, and may contribute to the adaptability of B. napus to P deficiency. Two functional markers, BnIPS2-C3 and BnGPT1-C3, which were developed from the genes AtIPS2 and AtGPT1 in Arabidopsis, were located in the confidence intervals of uq.C3a and uq.C3b, respectively. And AtGPT1 that corresponded to the interval of uq.C3b by in silico mapping was a possible candidate gene of uq.C3b. These results confirmed the importance of root traits for the adaptability of B. napus to LP and partially revealed the genetic basis of tolerance to P deficiency. These findings should be valuable for further study of the mechanism of P efficiency and the breeding of P-efficient cultivars by marker-assisted selection.

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Acknowledgments

This work was supported by grants from the National Basic Research and Development Program (2005CB120905), and the National 863 High Technology Program (2006AA10A112), China. The authors sincerely thank Prof. Xiaolong Yan and Prof. Hong Liao at the Root Biology Center, South China Agricultural University, for their kind help with the paper culture technique and the data analysis with WinRHIZO. We also thank the two anonymous reviewers for critical comments and valuable suggestions for revising the paper.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China

    Mei Yang, Guangda Ding, Lei Shi, Ji Feng, Fangsen Xu & Jinling Meng

  2. Key Laboratory of Subtropical Agricultural Resource and Environment, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, China

    Mei Yang, Guangda Ding, Lei Shi & Fangsen Xu

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  1. Mei Yang
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  2. Guangda Ding
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  4. Ji Feng
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Correspondence to Fangsen Xu.

Additional information

Communicated by H. Becker.

Electronic supplementary material

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122_2010_1301_MOESM1_ESM.xls

Supplementary material 1 (XLS 136 kb)Table S1a Framework map of 176 SSR markers constructed using the Brassica napus RIL population Table S1b Linkage map of 553 molecular markers constructed using the Brassica napus RIL population

122_2010_1301_MOESM2_ESM.doc

Supplementary material 2 (DOC 58 kb) Table S2 Pearson’s correlation coefficients between traits in the Brassica napus RIL population in the three experiments under high P (HP) and low P (LP) conditions

122_2010_1301_MOESM3_ESM.doc

Supplementary material 3 (DOC 138 kb) Table S3 All significant QTL detected by the composite interval method (CIM) at high P (HP) and low P (LP) conditions in the three independent experiments

122_2010_1301_MOESM4_ESM.doc

Supplementary material 4 (DOC 41 kb) Table S4 The phenotypic values under low P condition of the RI lines with contrary genotypes at the two loci of BnIPS2-C3 and BnGPT1-C3

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Yang, M., Ding, G., Shi, L. et al. Quantitative trait loci for root morphology in response to low phosphorus stress in Brassica napus . Theor Appl Genet 121, 181–193 (2010). https://doi.org/10.1007/s00122-010-1301-1

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  • DOI: https://doi.org/10.1007/s00122-010-1301-1

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