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Genetic dissection of the resistance to nine anthracnose races in the common bean differential cultivars MDRK and TU

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Abstract

Resistance to nine races of the pathogenic fungus Colletotrichum lindemuthianum, causal agent of anthracnose, was evaluated in F3 families derived from the cross between the anthracnose differential bean cultivars TU (resistant to races, 3, 6, 7, 31, 38, 39, 102, and 449) and MDRK (resistant to races, 449, and 1545). Molecular marker analyses were carried out in the F2 individuals in order to map and characterize the anthracnose resistance genes or gene clusters present in these two differential cultivars. The results of the combined segregation indicate that at least three independent loci conferring resistance to anthracnose are present in TU. One of them, corresponding to the previously described anthracnose resistance locus Co-5, is located in linkage group B7, and is formed by a cluster of different genes conferring specific resistance to races, 3, 6, 7, 31, 38, 39, 102, and 449. Evidence of intra-cluster recombination between these specific resistance genes was found. The second locus present in TU confers specific resistance to races 31 and 102, and the third locus confers specific resistance to race 102, the location of these two loci remains unknown. The resistance to race 1545 present in MDRK is due to two independent dominant genes. The results of the combined segregation of two F4 families showing monogenic segregation for resistance to race 1545 indicates that one of these two genes is linked to marker OF10530, located in linkage group B1, and corresponds to the previously described anthracnose resistance locus Co-1. The second gene conferring resistance to race 1545 in MDRK is linked to marker Pv-ctt001, located in linkage group B4, and corresponds to the Co-3/Co-9 cluster. The resistance to race 449 present in MDRK is conferred by a single gene, located in linkage group B4, probably included in the same Co-3/Co-9 cluster.

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Acknowledgments

This work was supported by grants AGL2004-08145-C02 and AGL2007-66563-C02 from the Ministerio de Educación y Ciencia, Spain. Ana Campa was recipient of a salary fellowship from the Instituto de Investigación y Tecnología Agraria y Alimentaria (INIA), Spain. Anthracnose races 7, 31, 39, 449, and 1545 were kindly provided by James D. Kelly (Michigan State University).

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Authors and Affiliations

  1. Area de Cultivos Hortofrutícolas y Forestales, SERIDA, Apdo. 13, 33300, Villaviciosa, Asturias, Spain

    Ana Campa & Juan José Ferreira

  2. Department of Biología Funcional, University of Oviedo, 33006, Oviedo, Spain

    Ramón Giraldez

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  1. Ana Campa
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  2. Ramón Giraldez
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  3. Juan José Ferreira
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Correspondence to Ramón Giraldez.

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Communicated by D. Hoisington.

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Campa, A., Giraldez, R. & Ferreira, J.J. Genetic dissection of the resistance to nine anthracnose races in the common bean differential cultivars MDRK and TU. Theor Appl Genet 119, 1–11 (2009). https://doi.org/10.1007/s00122-009-1011-8

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