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Molecular mapping and intra-cluster recombination between anthracnose race-specific resistance genes in the common bean differential cultivars Mexico 222 and Widusa

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Abstract

Resistance to races 19, 31, 38, 65, 73, 102, and 449, of the pathogenic fungus Colletotrichum lindemuthianum (anthracnose) was evaluated in F3 families derived from the cross between the anthracnose differential bean cultivars Mexico 222 (resistant to races 19, 31, and 38) and Widusa (resistant to races 38, 65, 73, 102, and 449). Molecular marker analyses were carried out in the corresponding F2 individuals in order to identify the genes for anthracnose resistance present in these two differential cultivars. The results of the combined segregation indicate that the resistance to anthracnose races 19, 31, and 38, present in Mexico 222, is conferred by single dominant race-specific genes organized in a cluster located in B4 linkage group, corresponding to the previously described Co-3/Co-9 locus. The resistance to anthracnose races 65, 73, 102, and 449, present in Widusa, is conferred by a dominant gene (or genes) representing a different haplotype of the same Co-3/Co-9 cluster. A single dominant gene located in a position independent from cluster Co-3/Co-9 (probably at the Co-1 locus) confers specific resistance to race 38 in Widusa. Recombinants for closely linked resistance specificities belonging to the Co-3/Co-9 cluster have been detected. The possibility of pyramiding race-specific resistance genes by means of intra-cluster recombination, and its potential use in plant breeding, is indicated.

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References

  • Alzate-Marin AL, Almeida KS, Barros EG, Moreira MA (2001) Preliminary results of allelism studies for anthracnose resistance genes of common bean cultivar PI 207262. Annu Rep Bean Improv Coop 44:113–114

    Google Scholar 

  • Alzate-Marin AL, Arruda KM, Barros EG, Moreira MA (2002) Allelism studies for anthracnose resistance genes of common bean cultivar Widusa. Annu Rep Bean Improv Coop 45:110–111

    Google Scholar 

  • Alzate-Marin AL, Souza KA, Morais Silva MG, Oliveira EJ, Moreira MA, Barros EG (2007) Genetic characterization of anthracnose resistance genes Co-4 3 and Co-9 in common bean cultivar tlalnepantla 64 (PI 207262). Euphytica 154:1–8

    Article  CAS  Google Scholar 

  • Bannerot H (1965) Résultats de l’infection d’une collection de haricots par six races physiologiques d’anthracnose. Ann Amélior Plantes 15:201–222

    Google Scholar 

  • Bannerot H, Derieux M, Fouilloux G (1971) Mise en évidence d’un second gène de résistance totale a l’anthracnose chez le haricot. Ann Amélior Plantes 21:83–85

    Google Scholar 

  • Campa A, Rodríguez-Suárez C, Pañeda A, Giraldez R, Ferreira JJ (2005) The bean anthracnose resistance gene Co-5, is located in linkage group B7. Annu Rep Bean Improv Coop 48:68–69

    Google Scholar 

  • Cardenas F, Adams MW, Andersen A (1964) The genetic system for reaction of field beans (Phaseolus vulgaris L.) to infection by three physiologic races of Colletotrichum lindemuthianum. Euphytica 13:178–186

    Google Scholar 

  • Creusot F, Macadré C, Ferrier-Cana E, Riou C, Geffroy V, Sévignac M, Dron M, Langin T (1999) Cloning and molecular characterization of three members of the NBS-LRR subfamily located in the vicinity of the Co-2 locus for anthracnose resistance in Phaseolus vulgaris. Genome 42:254–264

    Article  PubMed  CAS  Google Scholar 

  • Crute IR, Pink DAC (1996) Genetics and utilization of pathogen resistance in plants. Plant Cell 8:1747–1755

    Article  PubMed  CAS  Google Scholar 

  • Ferreira JJ, Rodríguez C, Pañeda A, Giraldez R (2003) Allelism test for resistance to race 38 of anthracnose in common bean differential cultivar, Widusa. Annu Rep Bean Improv Coop 46:169–170

    Google Scholar 

  • Ferrier-Cana E, Geffroy V, Macadré C, Creusot F, Imbert-Bolloré P, Sévignac M, Langin T (2003) Characterization of expressed NBS-LRR resistance gene candidates from common bean. Theor Appl Genet 106:251–261

    PubMed  CAS  Google Scholar 

  • Ferrier-Cana E, Macadré C, Sévignac M, David P, Langin T, Geffroy V (2005) Distinct post-transcriptional modifications result into seven alternative transcripts of the CC-NBS-LRR gene JA1tr of Phaseolus vulgaris. Theor Appl Genet 110:895–905

    Article  PubMed  CAS  Google Scholar 

  • Fouilloux G (1976) L’anthracnose du haricot (Colletotrichum lindemuthianum, Sacc et Magn): nouvelles sources de résistance et nouvelles races physiologiques. Ann Amélior Plantes 26:443–453

    Google Scholar 

  • Freyre R, Skroch PW, Geffroy V, Adam-Blondon A-F, Shirmohamadali A, Johnson WC, Llaca V, Nodari RO, Pereira PA, Tsai S-M, Tohme J, Dron M, Nienhuis J, Vallejos CE, Gepts P (1998) Towards an integrated linkage map of common bean. 4. Development of a core linkage map and alignment of RFLP maps. Theor Appl Genet 97:847–856

    Article  CAS  Google Scholar 

  • Geffroy V, Creusot F, Falquet J, Sévignac M, Adam-Blondon A-F, Bannerot H, Gepts P, Dron M (1998) A family of LRR sequences in the vicinity of the Co-2 locus for anthracnose resistance in Phaseolus vulgaris and its potential use in marker-assisted selection. Theor Appl Genet 96:494–502

    Article  CAS  Google Scholar 

  • Geffroy V, Sicard D, de Oliveira JCF, Sévignac M, Cohen S, Gepts P, Neema C, Langin T, Dron M (1999) Identification of an ancestral resistance gene cluster involved in the coevolution process between Phaseolus vulgaris and its fungal pathogen Colletotrichum lindemuthianum. Mol Plant Microbe Interact 12:774–784

    Article  PubMed  CAS  Google Scholar 

  • Geffroy V, Sévignac M, Billant P, Dron M, Langin T (2007) Resistance to Colletotrichum lindemuthianum in Phaseolus vulgaris: a case study for mapping two independent genes. Theor Appl Genet [Epub ahead of print]

  • Gonçalves-Vidigal MC, Kelly JD (2006) Inheritance of anthracnose resistance in the common bean cultivar Widusa. Euphytica 151:411–419

    Article  CAS  Google Scholar 

  • Kelly JD, Vallejo VA (2004) A comprehensive review of the major genes conditioning resistance to anthracnose in common bean. HortScience 39:1196–1207

    CAS  Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Marthur RS, Barnett HL, Lilly VG (1950) Sporulation of Colletotrichum lindemuthianum in culture. Phytopathology 40:104–114

    Google Scholar 

  • Melotto M, Kelly JD (2000) An allelic series at the Co-1 locus conditioning resistance to anthracnose in common bean of Andean origin. Euphytica 116:143–149

    Article  Google Scholar 

  • Melotto M, Kelly JD (2001) Fine mapping of the Co-4 locus of common bean reveals a resistance gene candidate, COK-4, that encodes for a protein kinase. Theor Appl Genet 103:508–517

    Article  CAS  Google Scholar 

  • Melotto M, Coelho MF, Pedrosa-Harand A, Kelly JD, Camargo LEA (2004) The anthracnose resistance locus Co-4 of common bean is located on chromosome 3 and contains putative disease resistance-related genes. Theor Appl Genet 109:690–699

    Article  PubMed  CAS  Google Scholar 

  • Méndez-Vigo B, Rodríguez-Suárez C, Pañeda A, Ferreira JJ, Giraldez R (2005) Molecular markers and allelic relationships of anthracnose resistance gene cluster B4 in common bean. Euphytica 141:237–245

    Article  CAS  Google Scholar 

  • Michelmore RW, Meyers BC (1998) Clusters of resistance genes in plants evolve by divergent selection and birth-and-death process. Genome Res 8:1113–1130

    PubMed  CAS  Google Scholar 

  • Miklas PN, Delorme R, Stone V, Daly MJ, Stavely JR, Steadman JR, Bassett MJ, Beaver JS (2000) Bacterial, fungal, and viral disease resistance loci mapped in a recombinant inbred common bean population (‘Dorado’/XAN 176). J Am Soc Hort Sci 125:476–481

    CAS  Google Scholar 

  • Muhalet CS, Adams MW, Saettler AW, Ghaderi A (1981) Genetic system for the reaction of field beans to beta, gamma, and delta races of Colletotrichum lindemuthianum. J Am Soc Hort Sci 106:601–604

    Google Scholar 

  • Pastor-Corrales MA (1991) Estandarización de variedades diferenciales y designación de razas de Colletotrichum lindemuthianum. Phytopathology 81:694

    Google Scholar 

  • Pastor-Corrales MA, Tu JC (1994) Antracnosis. In: Pastor-Corrales MA, Schwartz HF (eds) Problemas de producción del frijol en los trópicos. CIAT, Cali, pp 87–120

    Google Scholar 

  • Rodríguez-Guerra R, Ramírez-Rueda MT, Martínez de la Vega O, Simpson J (2003) Variation in genotype, pathotype and anastomosis groups of Colletotrichum lindemuthianum isolates from Mexico. Plant Pathol 52:228–235

    Article  Google Scholar 

  • Rodríguez-Suárez C, Méndez-Vigo B, Pañeda A, Ferreira JJ, Giraldez R (2007) A genetic linkage map of Phaseolus vulgaris L. and localization of genes for specific resistance to six races of anthracnose (Colletotrichum lindemuthianum). Theor Appl Genet 114:713–722

    Article  PubMed  CAS  Google Scholar 

  • Schwartz HF, Pastor-Corrales MA, Singh SP (1982) New sources of resistance to anthracnose and angular leaf spot of beans (Phaseolus vulgaris L.). Euphytica 31:741–754

    Article  Google Scholar 

  • Sharma KD, Winter P, Kahl G, Muehlbauer J (2004) Molecular mapping of Fusarium oxysporum f. sp. ciceris race 3 resistance gene in chickpea. Theor Appl Genet 108:1243–1248

    Article  PubMed  CAS  Google Scholar 

  • Van Schoonhoven A, Pastor-Corrales MA (1987) Standard system for the evaluation of bean germplasm. CIAT, Cali

    Google Scholar 

  • Vear F, Gentzbittel L, Philippon J, Mouzeyar S, Mestries E, Roeckel-Drevet P, Tourvieille de Labrouhe D, Nicolas P (1997) The genetics of resistance to five races of downy mildew (Plasmopara halstedii) in sunflower (Helianthus annuus L.). Theor Appl Genet 95:584–589

    Article  Google Scholar 

  • Young RA, Kelly JD (1996) Characterization of the genetic resistance to Colletotrichum lindemuthianum in common bean differential cultivars. Plant Dis 80:650–654

    Article  Google Scholar 

  • Young RA, Kelly JD (1997) RAPD markers linked to three major anthracnose resistance genes in common bean. Crop Sci 37:940–946

    Article  CAS  Google Scholar 

  • Young RA, Melotto M, Nodari RO, Kelly JD (1998) Marker-assisted dissection of the oligogenic anthracnose resistance in the common bean cultivar, ‘G2333’. Theor Appl Genet 96:87–94

    Article  CAS  Google Scholar 

  • Yu K, Park SJ, Poysa V, Gepts P (2000) Integration of simple sequence repeat (SSR) markers into a molecular linkage map of common bean (Phaseolus vulgaris L.). J Hered 91:429–434

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by grants AGL2001-2676-C02-01, RTA02-052-C2-2 and AGL2004-08145-C02-01 from the Ministerio de Ciencia y Tecnología, Spain. Cristina Rodríguez-Suárez was recipient of a salary fellowship from the Ministerio de Ciencia y Tecnología, Spain. Astrid Pañeda was recipient of salary fellowship from the Fundación para el Fomento de la Investigación Científica y Tecnológica (FICYT), Principado de Asturias, Spain. Ana Campa was recipient of a salary fellowship from the Instituto de Investigación y Tecnología Agraria y Alimentaria (INIA), Spain. Races 31, 65, 73, and 449 were kindly provided by James D. Kelly (Michigan State University).

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Authors and Affiliations

  1. Department of Biología Funcional, University of Oviedo, 33006, Oviedo, Spain

    Cristina Rodríguez-Suárez, Astrid Pañeda & Ramón Giraldez

  2. Area de Cultivos Hortofrutícolas y Forestales, SERIDA, Apdo. 13, 33300, Villaviciosa (Asturias), Spain

    Juan José Ferreira & Ana Campa

Authors
  1. Cristina Rodríguez-Suárez
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  2. Juan José Ferreira
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  3. Ana Campa
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  4. Astrid Pañeda
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  5. Ramón Giraldez
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Corresponding author

Correspondence to Ramón Giraldez.

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Communicated by F. J. Muehlbauer.

C. Rodríguez-Suárez and J.J. Ferreira equally share for authorship.

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Rodríguez-Suárez, C., Ferreira, J.J., Campa, A. et al. Molecular mapping and intra-cluster recombination between anthracnose race-specific resistance genes in the common bean differential cultivars Mexico 222 and Widusa. Theor Appl Genet 116, 807–814 (2008). https://doi.org/10.1007/s00122-008-0714-6

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  • DOI: https://doi.org/10.1007/s00122-008-0714-6

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