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Molecular mapping of quantitative trait loci for yield and yield components in spring wheat (Triticum aestivum L.)

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Abstract

An F1 derived doubled haploid (DH) population of 402 lines from the adapted spring wheat cross Superb (high yielding)/BW278 (low yielding) was developed to identify quantitative trait loci (QTL) associated with yield and yield components. A subset of the population (186 lines) was evaluated in replicated field trials in 2001 and 2002 at six locations in Manitoba and Saskatchewan, Canada. Agronomic parameters, grain yield and yield components including 1,000 grain weight, harvest index, average seed weight spike−1, seed number spike−1 and spikes number m−2 were measured. A genetic map was constructed with 268 microsatellite marker loci and included two morphological genes, reduced plant height, Rht-B1b, and the presence/absence of awns, B1. Composite interval mapping was conducted to estimate the location and effect of QTL associated with the evaluated traits. A total of 53 QTL were identified on 12 chromosomes for the 9 evaluated traits with the coefficient of determination ranging from 0.03 to 0.21 of the total variation. The increase in yield and yield components ranged from 4.5 to 17.1% over the population mean. The five grain yield QTL were detected on chromosomes 1A, 2D, 3B, and 5A and showed a combined increase of 34.4%, over the population mean. The alleles from Superb were associated with increased yield for four of the five QTL. This study identified potential chromosome segments for use in marker-assisted selection to improve yield and yield components in spring wheat.

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Acknowledgments

The authors wish to thank the CRC breeding crews and the Molecular Breeding lab for technical assistance with the field trials. The authors also thank Sheila Woods for statistical advice, Fred Townley-Smith for providing the DH population, and Colin Hiebert for identifying the source of the Rht-B1b gene. Funding for this research was provided by the Peter Dyck Fellowship, Western Grains Research Foundation, and Agriculture and Agri-Food Canada.

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Authors and Affiliations

  1. Department of Plant Science, University of Manitoba, Winnipeg, MB, Canada, R3T 2N2

    Janice L. Cuthbert & Anita L. Brûlé-Babel

  2. Cereal Research Centre, Agriculture and Agri-Food Canada, 195 Dafoe Rd, Winnipeg, MB, Canada, R3T 2M9

    Daryl J. Somers & P. Douglas Brown

  3. Department of Animal Science, University of Manitoba, Winnipeg, MB, Canada, R3T 2N2

    Gary H. Crow

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  1. Janice L. Cuthbert
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  2. Daryl J. Somers
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  3. Anita L. Brûlé-Babel
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  4. P. Douglas Brown
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  5. Gary H. Crow
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Correspondence to Daryl J. Somers.

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Communicated by C.-C. Schön.

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Cuthbert, J.L., Somers, D.J., Brûlé-Babel, A.L. et al. Molecular mapping of quantitative trait loci for yield and yield components in spring wheat (Triticum aestivum L.). Theor Appl Genet 117, 595–608 (2008). https://doi.org/10.1007/s00122-008-0804-5

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