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Detection of QTLs for Stagonospora glume blotch resistance in Swiss winter wheat

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Abstract

Stagonospora nodorum is the causal agent of the Stagonospora glume blotch disease in hexaploid wheat. The Swiss winter bread wheat cv. 'Arina' has a highly effective, durable and quantitative glume blotch resistance. We studied 240 single seed descent (SSD)-derived lines of an 'Arina × Forno' F5:7 population to identify and map quantitative trait loci (QTLs) for glume blotch resistance under natural infestation. Using composite interval mapping (CIM) and LOD>4.5, we detected two chromosomal regions on chromosome arms 3BS and 4BL which were specifically associated with glume blotch resistance. These identified QTLs were designated QSng.sfr-3BS and QSng.sfr-4BL, respectively. QSng.sfr-3BS peaked at the locus Xgwm389 in the telomeric region of the short arm of chromosome 3B and explained 31.2% of the observed phenotypic variance for the resistance within the population. The responsible QSng.sfr-3BS allele originated from the resistant parent 'Arina'. The QTL QSng.sfr-4BL (19.1%) mapped to chromosome arm 4BL ('Forno' allele) very close to two known genes, TaMlo and a catalase (Cat). Both QTL alleles combined could enhance the resistance level by about 50%. Additionally, they showed significant epistatic effects (4.4%). We found PCR-based microsatellite markers closely linked to QSng.sfr-3BS (gwm389) and QSng.sfr-4BL (gwm251) which make marker-assisted selection (MAS) for Stagonospora glume blotch resistance feasible. We also found one resistance QTL, QSng.sfr-5BL, on the long arm of chromosome 5B which overlapped with QTLs for plant height as well as heading time.

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Acknowledgements

We thank B. Senger and P. Streckeisen for their excellent technical assistance, and R. Enderlin for conducting the field trials in Haag, Canton St. Gallen, Switzerland. We are grateful to Prof. Dr. H.F. Utz for providing the computer packages PLABSTAT and PLABQTL. This work was supported by the University of Zürich. The authors declare that the experiments conducted for this publication comply with the current laws of Switzerland.

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Authors and Affiliations

  1. Institute of Plant Biology, University of Zürich, Zollikerstrasse 107, 8008, Zürich, Switzerland

    T. Schnurbusch & B. Keller

  2. Swiss Federal Research Station for Agroecology and Agriculture (FAL), Reckenholzstrasse 191, 8046, Zürich, Switzerland

    S. Paillard, G. Schachermayr & M. Winzeler

  3. Swiss Federal Research Station for Plant Production, Changins, Route de Duillier, P.O. Box 254, 1260 1, Nyon, Switzerland

    D. Fossati

  4. Pharmaceutical Institute, University of Basel, Benkenstrasse 254, 4108, Witterswil, Switzerland

    M. Messmer

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  1. T. Schnurbusch
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Correspondence to B. Keller.

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Communicated by H. C. Becker

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Schnurbusch, T., Paillard, S., Fossati, D. et al. Detection of QTLs for Stagonospora glume blotch resistance in Swiss winter wheat. Theor Appl Genet 107, 1226–1234 (2003). https://doi.org/10.1007/s00122-003-1372-3

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  • DOI: https://doi.org/10.1007/s00122-003-1372-3

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