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Fine mapping of the Pc locus of Sorghum bicolor, a gene controlling the reaction to a fungal pathogen and its host-selective toxin

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Abstract

Milo disease in sorghum is caused by isolates of the soil-borne fungus Periconia circinata that produce PC-toxin. Susceptibility to milo disease is conditioned by a single, semi-dominant gene, termed Pc. The susceptible allele (Pc) converts to a resistant form (pc) spontaneously at a gametic frequency of 10−3 to 10−4. A high-density genetic map was constructed around the Pc locus using DNA markers, allowing the Pc gene to be delimited to a 0.9 cM region on the short arm of sorghum chromosome 9. Physically, the Pc-region was covered by a single BAC clone. Sequence analysis of this BAC revealed twelve gene candidates. Several of the predicted genes in the region are homologous to disease resistance loci, including one NBS-LRR resistance gene analogue that is present in multiple tandem copies. Analysis of pc isolines derived from Pc/Pc sorghum suggests that one or more members of this NBS-LRR gene family are the Pc genes that condition susceptibility.

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Acknowledgments

This article is dedicated to the memory of the late Dr. Keith Schertz, a much-valued and -missed colleague, mentor and friend. This work was supported by Umbarger and Giles Chair endowments to JLB. The technical assistance of Trudi Thomas and Matt Estep is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Genetics, University of Georgia, Athens, GA, 30602, USA

    Ervin D. Nagy & Jeffrey L. Bennetzen

  2. Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN, 47907, USA

    Tso-Ching Lee

  3. Department of Biological Sciences, Michigan Tech University, Houghton, MI, 49931, USA

    Wusirika Ramakrishna & Zijun Xu

  4. Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, TX, 77843, USA

    Patricia E. Klein

  5. Agricultural Genomics Center, Purdue University, West Lafayette, IN, 47907, USA

    Phillip SanMiguel

  6. Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907, USA

    Chiu-Ping Cheng & Jingling Li

  7. Department of Crop and Soil Sciences and Department of Plant Biology, University of Georgia, Athens, GA, 30602, USA

    Katrien M. Devos

  8. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX, 77843, USA

    Keith Schertz

  9. Crop Production and Pest Control Research, USDA-ARS, West Lafayette, IN, 47907, USA

    Larry Dunkle

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  1. Ervin D. Nagy
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  10. Keith Schertz
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  11. Larry Dunkle
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  12. Jeffrey L. Bennetzen
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Correspondence to Jeffrey L. Bennetzen.

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Communicated by F. Ordon.

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Nagy, E.D., Lee, TC., Ramakrishna, W. et al. Fine mapping of the Pc locus of Sorghum bicolor, a gene controlling the reaction to a fungal pathogen and its host-selective toxin. Theor Appl Genet 114, 961–970 (2007). https://doi.org/10.1007/s00122-006-0481-1

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  • DOI: https://doi.org/10.1007/s00122-006-0481-1

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