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Genetic and physical mapping of sequence-specific amplified polymorphic (SSAP) markers on the 1RS chromosome arm of rye in a wheat background

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Abstract

Three rye-specific repeated sequences, pSc10C, pSc20H and R173-1, were used to design sequence-specific anchored primers. These primers and 16 restriction site-specific adaptor primers were used in all possible combinations to establish sequence-specific amplified polymorphic (SSAP) markers for the 1RS chromosome arm of rye in a wheat background. Thirty 1RS-specific SSAP markers were detected in 19 primer combinations. Along with six markers localised previously on 1RS, 26 of the SSAP markers were mapped genetically in wheat genotypes carrying recombinant 1BL.1RS translocations. A clear decrease in recombination frequency from distal to proximal regions was observed. Wheat-rye addition lines for the 1R chromosome with different-sized deletions of the short arm were used to physically localise these markers. Physical mapping suggested an even distribution of the SSAP markers along the total length of the 1RS chromosome arm.

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Acknowledgements

This work was financially supported by the Austrian Science Fund (FWF, No. P13852-BIO). The authors are grateful to Dr. Mária Berényi and Dr. Kornél Burg (Austrian Research Center, Seibersdorf) for their advices, and critical comments on the manuscript. The CS/I addition lines were kindly provided by Dr. Bernd Friebe (Kansas State University, USA). The primary triticales were developed by Dr. Gabriella Linc in Martonvásár. The technical assistance of Gertraud Stift is also acknowledged. Thanks are due to Barbara Harasztos for revising the manuscript linguistically.

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Authors and Affiliations

  1. Agricultural Research Institute of the Hungarian Academy of Sciences, Martonvásár, 2462, Brunszvik 2, Hungary

    E. D. Nagy

  2. Institute for Agrobiotechnology, University of Natural Resources and Applied Life Sciences, Konrad Lorenz 20, 3430, Tulln, Austria

    T. Lelley

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  1. E. D. Nagy
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  2. T. Lelley
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Correspondence to T. Lelley.

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Communicated by J.W. Snape

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Nagy, E.D., Lelley, T. Genetic and physical mapping of sequence-specific amplified polymorphic (SSAP) markers on the 1RS chromosome arm of rye in a wheat background. Theor Appl Genet 107, 1271–1277 (2003). https://doi.org/10.1007/s00122-003-1367-0

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  • DOI: https://doi.org/10.1007/s00122-003-1367-0

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