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A perennial ryegrass CBF gene cluster is located in a region predicted by conserved synteny between Poaceae species

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Abstract

CBF/DREB1 proteins are the most important regulators of the cold temperature signaling pathway in many plants. CBF genes are candidates for low-temperature tolerance QTL in wheat and barley. Ten novel putative CBF cDNAs of perennial ryegrass (Lolium perenne L.) have been isolated from cold-treated leaf tissue. Their primary structures contain some conserved motifs, characteristic of the gene class. Phylogenetic analysis revealed that LpCBF genes were attributable to the HvCBF3-, and HvCBF4-subgroups following the previously proposed classification of barley CBF genes. RT-PCR analysis revealed that the expression of LpCBF genes was rapidly induced in response to low temperature and that the expression pattern under the low-temperature conditions for a long period was different between the various LpCBF genes. Five of the ten LpCBF genes were assigned to the genetic linkage map using the p150/112 reference mapping population. LpCBFIb, LpCBFII, LpCBFIIIb and LpCBFIIIc were mapped on LG5 forming a cluster within 2.2 cM, while LpCBFVb was located on LG1. Based on comparative genetic studies, conserved synteny for CBF gene family was observed between the Triticeae cereals and perennial ryegrass. Information on the perennial ryegrass CBF genes at both the molecular and genetic level obtained in this study would be useful for the further study on the role of CBF genes and low-temperature tolerance in grasses.

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Acknowledgment

We gratefully acknowledge Dr. Sohei Kobayashi, Dr. Kazuhiro Tase and Mr. Yasuharu Sanada for their technical advice, and Miss Mayumi Hata and Miss Mayuko Kawamura for their technical assistance. This work was supported, in part, by Grants-in Aid for Scientific Research (No. 17380163) from the Ministry of Education, Science, Sports and Culture, Japan. The authors thank Prof. Michael Hayward (Rhydgoch Genetics, Aberystwyth, UK) and Dr. Toshinori Komatsu for careful critical reading of the manuscript.

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  1. National Agricultural Research Center for Hokkaido Region, National Agriculture and Food Research Organization, Hitsujigaoka, Toyohira, Sapporo, 062-8555, Japan

    K. Tamura

  2. Field Science Center for Northern Biosphere, Hokkaido University, Kita 11, Nishi 10, Kita, Sapporo, 060-0811, Japan

    T. Yamada

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  1. K. Tamura
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Correspondence to T. Yamada.

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Communicated by H. H. Geiger.

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Tamura, K., Yamada, T. A perennial ryegrass CBF gene cluster is located in a region predicted by conserved synteny between Poaceae species. Theor Appl Genet 114, 273–283 (2007). https://doi.org/10.1007/s00122-006-0430-z

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  • DOI: https://doi.org/10.1007/s00122-006-0430-z

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