Abstract
A population of single chromosome recombinant lines was developed from the cross between a frost-sensitive, vernalization-insensitive substitution line, ‘Chinese Spring’ (Triticum spelta 5A) and a frost-tolerant, vernalization-sensitive line, ‘Chinese Spring’ (‘Cheyenne’ 5A), and used to map the genes Vrn1 and Fr1 controlling vernalization requirement and frost tolerance, respectively, relative to RFLP markers located on this chromosome. The Vrn1 and Fr1 loci were located closely linked on the distal portion of the long arm of 5AL, but contrary to previous observations, recombination between them was found. Three RFLP markers, Xpsr426, Xcdo504 and Xwg644 were tightly linked to both. The location of Vrn1 suggests that it is homoeologous to other spring habit genes in related species, particularly the Sh2 locus on chromosome 7 (5H) of barley and the Sp1 locus on chromosome 5R of rye.
Similar content being viewed by others
References
Ainsworth CC, Miller TE, Gale MD (1987) α-Amylase and β-amylase homoeoloci in species related to wheat. Genet Res 49:93–103
Brule-Babel AL, Fowler DB (1988) Genetic control of cold hardiness and vernalization requirement in winter wheat. Crop Sci 28:879–884
Cahalan C, Law CN (1979) The genetical control of cold resistance and vernalization requirement in wheat. Heredity 42:125–132
Chojecki J, Barnes S, Dunlop A (1989) A molecular marker for vernalization requirement in barley. In: Helentjaris T, Burr B (eds) Development and application of molecular markers to problems in plant genetics (Current Communications in molecular biology). Cold Spring Harbour Laboratory Press, Cold Spring Harbour, N.Y., pp 145–148
Devos KM, Gale MD (1993) The genetic maps of wheat and their potential in plant breeding. Outlook Agric 22:93–99
Devos KM, Atkinson MD, Chinoy CN, Liu CJ, Gale MD (1992) RFLP-based genetic map of the homoeologous group 3 chromosomes of wheat and rye. Theor Appl Genet 83:931–939
Devos KM, Atkinson MD, Chinoy CN, Francis HA, Harcourt RL, Koebner RMD, Liu CJ, Masojc P, Xie DX, Gale MD (1993) Chromosomal rearrangements in the rye genome relative to that of wheat. Theor Appl Genet 85:673–680
De Vries JN, Sybenga J (1984) Chromosomal location of 17 monogenically inherited morphological markers in rye (Secale cereale L.) using the translocation tester set. Z Pflanzenzuecht 92:117–139
Forster BP, Ellis RP (1991) Two biochemical markers for spring/winter habit. In: Munck L, Kirkegaard K, Jensen B (eds) Barley Genetics VI. Proc 6th Int Barley Genet Symp. Munksgaard International Publ, Copenhagen pp 101–103
Galiba G, Sutka J (1988) A genetic study of frost resistance in wheat callus culture. Plant Breed 101: 132–136
Hackett CA, Ellis RP, Foster BP, McNicol JW, Macaulay M (1992) Statistical analysis of a linkage experiment in barley involving quantitative trait loci for height and ear-emergence time and two genetic markers on chromosome 4. Theor Appl Genet 85: 120–126
Hayes PM, Blake T, Chen THH, Tragoonrung S, Chen F, Pan A, Liu B (1993) Quantitative trait loci on barley (Hordeum vulgare L.) chromosome 7 associated with components of winter hardiness. Genome 36:66–71
Heun M, Kennedy AE, Anderson JA, Lapitan NLV, Sorrells ME, Tanksley SD (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare). Genome 4:437–447
Kleinhofs A, Killian A, Saghai Maroof MA, Biyashev RM, Hayes P, Chen FQ, Lapitan N, Fenwick A, Blake TK, Kanazin V, Ananiev E, Dahleen L, Kudrna D, Bollinger D, Knapp SJ, Liu B, Sorrells M, Heun M, Franckowiak JD, Hoffmann D, Skadsen R, Steffenson BJ (1993) A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theor Appl Genet 86:705–712
Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175
Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121: 185–199
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Laurie DA, Pratchett N, Bezant J, Snape JW (1995) RFLP mapping of 13 loci controlling flowering time in a winter x spring barley (Hordeum vulgare L.) cross. Genome (in press)
Law CN (1966) The locations of genetic factors affecting a quantitative character in wheat. Genetics 53:487–498
Law CN, Jenkins G (1970) A genetic study of cold resistance in wheat. Genet Res 15:197–208
Law CN, Worland AJ, Giorgi B (1976) The genetic control of earemergence time by chromosomes 5A and 5D of wheat. Heredity 36:49–58
Law CN, Snape JW, Worland AJ (1981) Intraspecific chromosome manipulation. Philos Trans R Soc London Ser B 292:509–518
Liu CJ, Atkinson MD, Chinoy CN, Devos KM, Gale MD (1992) Nonhomoeologous translocations between group 4, 5 and 7 chromosomes within wheat and rye. Theor Appl Genet 83:305–312
Melz G (1989) Beiträge zur genetik des roggens (Secale cereale L.) DSc thesis, Berlin
Murray MG, Thompson WF (1980) The isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325
Plaschke J, Börner A, Xie DX, Koebner RMD, Schlegel R, Gale MD (1993) RFLP mapping of genes affecting plant height and growth habit in rye. Theor Appl Genet 85:1049–1054
Puchkov YM, Zhirov EG (1978) Breeding of common wheat varieties with a high frost resistance and genetic aspects of it. World Sci News 15:17–22
Pugsley AT (1971) A genetic analysis of the spring-winter habit in wheat. Aust J Agric Res 22:21–31
Roberts DWA (1986) Chromosomes in ‘Cadet’ and ‘Rescue’ wheats carrying loci for cold hardiness and vernalization response. Can J Genet Cytol 28:991–997
Roberts DWA (1989) Identification of loci on chromosome 5A of wheat involved in control of cold hardiness, vernalization, leaf length, rosette growth habit, and height of hardened plants. Genome 33:247–259
Snape JW, Law CN, Worland AJ (1976) Chromosome variation for loci controlling ear-emergence time on chromosome 5A of wheat. Heredity 37:335–340
Snape JW, Law CN, Parker BB, Worland AJ (1985) Genetical analysis of chromosome 5A of wheat and its influence on important agronomic characters. Theor Appl Genet 71:518–526
Sogaard B, Von Wettstein-Knowles P (1987) Barley: genes and chromosomes. Carlsberg Res Commun 52:123–196
Sutka J (1981) Genetic studies of frost resistance in wheat. Theor Appl Genet 59:145–152
Sutka J, Snape JW (1989) Location of a gene for frost resistance on chromosome 5A of wheat. Euphytica 42:41–44
Takahashi R, Yasuda S (1971) Genetics of earliness and growth habit in barley. In: Nilan RA (ed) Barley Genetics II. Proc 2nd Int Barley Genet Symp. Washington State University Press, Seattle, Wash., pp 388–408
Veisz O, Sutka J (1989) The relationships of hardening period and expression of frost resistance in chromosome substitution lines of wheat. Euphytica 43:41–45
Worland AJ, Gale MD, Law CN (1987) Wheat genetics. In: Lupton FGH (ed) Wheat breeding. Chapman and Hall, New York, pp 129–171
Wricke G (1991) A molecular marker linkage map of rye for plant breeding. Vortr Pflanzenzuecht 20:72–78
Xie DX, Devos KM, Moore G, Gale MD (1993) RFLP-based genetic maps of the homoeologous group 5 chromosomes of bread wheat (Triticum aestivum L.). Theor Appl Genet 87:70–74
Author information
Authors and Affiliations
Additional information
Communicated by G. Wenzel
Rights and permissions
About this article
Cite this article
Galiba, G., Quarrie, S.A., Sutka, J. et al. RFLP mapping of the vernalization (Vrn1) and frost resistance (Fr1) genes on chromosome 5A of wheat. Theoret. Appl. Genetics 90, 1174–1179 (1995). https://doi.org/10.1007/BF00222940
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00222940