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Genetic dissection and validation of QTLs for grain shape and weight in rice and fine mapping of qGL1.3, a major QTL for grain length and weight

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Abstract

Grain shape is a key factor that influences both grain yield and quality of rice (Oryza sativa L.). In order to elucidate the genetic basis of grain shape, an F2 and derived F2:3 populations were developed from a cross between Guangzhan63-4S (GZ63-4S) and TGMS29, and used in a quantitative trait locus (QTL) analysis. A total of thirty-six QTLs were identified for grain shape and weight, and the phenotypic variance explained by each QTL ranged from 0.88 to 35.70%. Given that most of the QTLs were detected in only one population, a BC1F2 and derived BC1F2:3 populations were constructed with GZ63-4S as the recurrent parent to further verify the QTLs. Seventeen QTLs were validated in at least one population. qGL1.3, the major QTL for grain length in both the F2 and F2:3 populations, contributed 41.16% of the variation in grain length and 35.96% of the variation in 1000-grain weight in a random BC1F3 population, and thus was selected for further fine mapping. Based on progeny tests of 31 recombinants, qGL1.3 was delimited to a region of about 350 kb on chromosome 1. These results could lay the foundation for map-based cloning of qGL1.3 and provide genes or QTLs for breeding rice lines with high grain yield and quality.

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Funding

This work was supported by grants from the National Program on R&D of Transgenic Plants (2016ZX08009003-004) and the National Natural Science Foundation (91935303), and earmarked fund for Agriculture Research System (CARS-01-03) in China.

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Author notes

  1. Yin Zhou and Jun Hou contributed equally to this work.

Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement and National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan, 430070, Hubei, China

    Yin Zhou, Jun Hou, Pingbo Li, Hanyuan Yang, Duo Xia, Hao Zhou, Mufid Alam, Guanjun Gao, Qinglu Zhang & Yuqing He

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  1. Yin Zhou
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  2. Jun Hou
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  3. Pingbo Li
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  6. Hao Zhou
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Correspondence to Yuqing He.

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Zhou, Y., Hou, J., Li, P. et al. Genetic dissection and validation of QTLs for grain shape and weight in rice and fine mapping of qGL1.3, a major QTL for grain length and weight. Mol Breeding 39, 170 (2019). https://doi.org/10.1007/s11032-019-1079-z

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