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The role of nitric oxide during healing of trauma to the skeletal muscle

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Abstract

Introduction

The role of NO in muscle injury is not clear.

Methods

We examined the involvement of the NO system in the development of muscle damage in an experimental model of crush injury. The animals were divided into four groups: (1) control (CO), (2) sham trauma, (3) trauma, (4) trauma + l-NAME, in two experimental phases, 24 h and 7 days after injury.

Results

Twenty-four hours post-trauma, the crushed muscle was characterized by an intense inflammatory reaction. These changes were accompanied by increased oxidative damage, increased cytokine mRNA transcription, NF-κB binding ability and TGF-β growth factor expression in the gastrocnemius muscle. Treatment with l-NAME markedly decreased these histological and molecular abnormalities at 24 h. However, at 7 days post-trauma, increased collagen formation was observed in the l-NAME group.

Discussion

These findings indicate that NO is involved in the balance between fibrosis and healing with regeneration.

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Acknowledgments

Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundo de Incentivo a Pesquisa e Eventos (FIPE).

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Authors and Affiliations

  1. Serviço de Reumatologia, Laboratory of Molecular Biology of Autoimmune and Infectious Disease, Hospital de Clínicas de Porto Alegre (HCPA), Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos 2350, 6º andar/sala645, Porto Alegre, RS, 90035-003, Brazil

    Lidiane Isabel Filippin & Ricardo Machado Xavier

  2. Institute of Biomedicine (IBIOMED), University of Leon, Leon, Spain

    María José Cuevas, Elena Lima & Javier Gonzalez-Gallego

  3. Experimental Hepatology and Physiology Laboratory of HCPA, Porto Alegre, Brazil

    Norma Possa Marroni

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  1. Lidiane Isabel Filippin
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  2. María José Cuevas
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  3. Elena Lima
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  4. Norma Possa Marroni
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  5. Javier Gonzalez-Gallego
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  6. Ricardo Machado Xavier
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Correspondence to Ricardo Machado Xavier.

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Responsible Editor: Artur Bauhofer.

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Filippin, L.I., Cuevas, M.J., Lima, E. et al. The role of nitric oxide during healing of trauma to the skeletal muscle. Inflamm. Res. 60, 347–356 (2011). https://doi.org/10.1007/s00011-010-0277-2

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  • DOI: https://doi.org/10.1007/s00011-010-0277-2

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