Abstract
A detailed study on the newer methods of coupling urease to diverse matrices have been investigated towards optimizing the efficiency of the immobilized enzyme action and cost effectiveness for the industrial applications. With some of the matrices like collagen, DEAE-cellulose and nylon, even the crude extract of jack bean meal, a major and inexpensive form of urease has been found to be extremely efficient and has indicated that factors other than covalent bonding contribute to the enzyme immobilization. Increased flow rate of the substrate has also resulted in increased reaction kinetics. Apparently, the rapid removal of the product, ammonium ion is largely responsible for this effect. Based on these informations, a large scale column with nylon net has been tested for its efficiency in handling urea effluent from fertilizer plants.
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George, S., Chellapandian, M., Sivasankar, B. et al. Flow rate dependent kinetics of urease immobilized onto diverse matrices. Bioprocess Engineering 15, 311–315 (1996). https://doi.org/10.1007/BF02426440
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DOI: https://doi.org/10.1007/BF02426440