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Immobilization of urease on gelatin — poly (HEMA) copolymer preparation and characterization

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Abstract

Urease was immobilized onto gelatin-poly (HEMA) copolymer by covalent linkage. Maximum amount of urease was immobilized onto the support at a pH of 8.5. The optimal pH of the immobilized urease was similar to that of free urease; the optimal temperature showed an increase of 10 °C over the free enzyme. The stability of the immobilized urease for a range of pH, temperature and shelf life was greater than the corresponding values for the free enzyme. The same result was obtained for k m also.

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Authors and Affiliations

  1. Department of Chemical Engineering, Anna University, 600025, Madras, India

    M. Srinivasa Rao, M. Chellapandian & M. R. V. Krishnan

Authors
  1. M. Srinivasa Rao
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  2. M. Chellapandian
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  3. M. R. V. Krishnan
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Additional information

Grateful acknowledgement is made to CSIR, Govt. of India for the research associateship conferred on Dr. M. Chellapandian which helped the progress of this piece of research investigation.

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Srinivasa Rao, M., Chellapandian, M. & Krishnan, M.R.V. Immobilization of urease on gelatin — poly (HEMA) copolymer preparation and characterization. Bioprocess Engineering 13, 211–214 (1995). https://doi.org/10.1007/BF00367256

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  • DOI: https://doi.org/10.1007/BF00367256

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