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Control of Elevated Ion Accumulation, Oxidative Stress, and Lipid Peroxidation with Salicylic Acid-Induced Accumulation of Glycine Betaine in Salinity-Exposed Vigna radiata L

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Abstract

The identification of the sustainable approaches is required for the minimization of adverse impact of worldwide increasing soil salinity on plant growth, development, and productivity. This study investigated the protective role and major mechanism underlying salicylic acid (SA; 0.1, 0.5, or 1.0 mM)-induced glycine betaine (GB)-mediated tolerance to salinity (50 mM NaCl) in mungbean (Vigna radiata L. cultivar Punt Mung). The supply of 0.5 mM SA maximally increased the accumulation of GB (>40%) with respect to the control. This was further corroborated with the increase in water potential, antioxidant system (reduced glutathione (GSH), GSH/GSSG redox state, and glutathione reductase (GR) activity) and decreased Na+ and Cl− accumulation, Na+/K+ ratio, oxidative stress, and lipid peroxidation. This was also associated with the increased photosynthesis (14–18%) and growth (7–12%) parameters. Overall, SA-induced accumulation of GB protected photosynthesis and growth against 50 mM NaCl-accrued impacts in V. radiata through minimizing the accumulation of Na+ and Cl− ions, oxidative stress, and maintaining high GSH level that led to reduced cellular redox environment.

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Acknowledgements

Author (AM) acknowledges the Department of Science and Technology, Government of India, New Delhi for the grant SERB.LS-184/2014.

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Authors and Affiliations

  1. Plant Physiology and Biochemistry Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, 202002, India

    Shabina Syeed, Zebus Sehar, Asim Masood, Naser A. Anjum & Nafees A. Khan

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  1. Shabina Syeed
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  2. Zebus Sehar
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  3. Asim Masood
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  4. Naser A. Anjum
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  5. Nafees A. Khan
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Asim Masood: experimentation, methodology, writing original draft; Zebus Sehar: experimentation, data analyses; Naser Anjum: formal analysis, computational work; Shabina Syeed: data analyses, methodology; Nafees Khan: supervision, conceptualization.

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Correspondence to Asim Masood or Nafees A. Khan.

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Shabina Syeed, Zebus Sehar, and Asim Masood share first authorship.

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Syeed, S., Sehar, Z., Masood, A. et al. Control of Elevated Ion Accumulation, Oxidative Stress, and Lipid Peroxidation with Salicylic Acid-Induced Accumulation of Glycine Betaine in Salinity-Exposed Vigna radiata L. Appl Biochem Biotechnol 193, 3301–3320 (2021). https://doi.org/10.1007/s12010-021-03595-9

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