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Characterization of ω-secalin genes from rye, triticale, and a wheat 1BL/1RS translocation line

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Abstract

Sixty-two DNA sequences for the coding regions of omega-secalin (ω-secalin) genes have been characterized from rye (Secale cereale L.), hexaploid and octoploid triticale (×Triticosecale Wittmack), and wheat (Triticum aestivum L.) 1BL/1RS translocation line. Only 19 out of the 62 ω-secalin gene sequences were full-length open reading frames (ORFs), which can be expressed into functional proteins. The other 43 DNA sequences were pseudogenes, as their ORFs were interrupted by one or a few stop codons or frameshift mutations. The 19 ω-secalin genes have a typical primary structure, which is different from wheat gliadins. There was no cysteine residue in ω-secalin proteins, and the potential celiac disease (CD) toxic epitope (PQQP) was identified to appear frequently in the repetitive domains. The ω-secalin genes from various cereal species shared high homology in their gene sequences. The ω-secalin gene family has involved fewer variations after the integration of the rye R chromosome or whole genome into the wheat or triticale genome. The higher Ka/Ks ratio (i.e. non-synonymous to synonymous substitutions per site) in ω-secalin pseudogenes than in ω-secalin ORFs indicate that the pseudogenes may be subject to a reduced selection pressure. Based on the conserved sequences of ω-secalin genes, it will be possible to manipulate the expression of this gene family in rye, triticale, or wheat 1BL/1RS translocation lines, to reduce its negative effects on grain quality.

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Authors and Affiliations

  1. Triticeae Research Institute, Sichuan Agricultural University, 625014, Ya’an, Sichuan, China

    Q -T. Jiang, Y -M. Wei, Z -E. Pu, Y -Y. Peng & Y -L. Zheng

  2. Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada

    Q -T. Jiang, L. Andre & Z -X. Lu

  3. Key Laboratory of Southwestern Crop Germplasm Utilization, Ministry of Agriculture, Ya’an, Sichuan, China

    Q -T. Jiang & Y -L. Zheng

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  1. Q -T. Jiang
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  2. Y -M. Wei
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  3. L. Andre
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  4. Z -X. Lu
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  5. Z -E. Pu
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  6. Y -Y. Peng
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  7. Y -L. Zheng
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Correspondence to Y -L. Zheng.

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These authors contributed equally to this study

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Jiang, Q.T., Wei, Y.M., Andre, L. et al. Characterization of ω-secalin genes from rye, triticale, and a wheat 1BL/1RS translocation line. J Appl Genet 51, 403–411 (2010). https://doi.org/10.1007/BF03208870

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  • DOI: https://doi.org/10.1007/BF03208870

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