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A new α-gliadin gene family for wheat breeding: somatic introgression line II-12 derived from Triticum aestivum and Agropyron elongatum

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Abstract

Ninety-five α-gliadin open reading frames were cloned and sequenced from the somatic hybrid wheat introgression line II-12 and its parents Triticum aestivum cv. Jinan177 (JN177) and Agropyron elongatum. Novel α-gliadin genes were found to originate via point mutation, unequal crossover or slippage of a parental gene, demonstrating that new genes could be rapidly created through somatic hybridization in a manner similar to that previously shown for high-molecular-weight glutenin subunits (HMW-GS) genes. The data reveal the composition and origin of the α-gliadin gene in II-12, showing that: (1) most were homologous to those of JN177; (2) a few were derived direct from A. elongatum; and (3) some new genes were created de novo. A particular quality attribute of interest was the presence or absence of celiac disease (CD) epitopes, which were found to be four times more common among α-gliadin genes from the parent wheat JN177 than in those from A. elongatum. Although four types of CD epitopes were found in introgression line II-12, the number of genes encoded CD epitopes was lower than in JN177 due to the occurrence of pseudogenes. We discuss the benefit of these α-gliadins to wheat breeding.

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Acknowledgements

We are grateful to Prof. Rudi Appels (Centre for Comparative Genomics, Murdoch University, Perth WA 6150, Australia) for many significant suggestions and correction. This work was supported by the National 863 High Technology Research and Development Project 2006AA10Z173 and 2006011001020, Key and Doctor-Site Foundation of Ministry of Education in China, Natural Science Foundation of Shandong Province No. Y2007D48.

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Authors and Affiliations

  1. School of Life Science, Shandong University, Jinan, 250100, People’s Republic of China

    Fanguo Chen, Chunhui Xu, Mengzhu Chen, Yanhui Wang & Guangmin Xia

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  1. Fanguo Chen
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  2. Chunhui Xu
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  3. Mengzhu Chen
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  4. Yanhui Wang
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  5. Guangmin Xia
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Corresponding author

Correspondence to Guangmin Xia.

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Fanguo Chen, Chunhui Xu, and Mengzhu Chen contributed equally to this work.

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Chen, F., Xu, C., Chen, M. et al. A new α-gliadin gene family for wheat breeding: somatic introgression line II-12 derived from Triticum aestivum and Agropyron elongatum . Mol Breeding 22, 675–685 (2008). https://doi.org/10.1007/s11032-008-9208-0

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