Summary
A sensitive histochemical technique has been used to visualize the ultrastructural localization of mercury in the anterior pituitary of rats which have been exposed to methyl mercury. After administration of methyl mercury in the drinking water (20 mg × 1−1 methyl mercury in distilled water) or intraperitoneally (daily dose 100 ug or 200 ug methyl mercury) intracellular accumulations of mercury were found in the lysosomes and granules of secretory cells (somatotrophs, thyrotrophs and corticotrophs). In non-secretory cells (follicular cell and marginal layer cells) mercury deposits were found in lysosomes. In orally treated rats, the number of mercury deposits increased significantly with time up to day 21. In rats exposed intraperitoneally, a continuous increase was seen in intracellular mercury accumulation. Apart from vacuolation of lysosomes, no structural damage was observed in the cells containing mercury.
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References
Berlin M, Ullberg S (1963a) Accumulation and retention of mercury in the mouse. II. An autoradiographic comparison of phenyl mercuric acetate with inorganic mercury. Arch Environ Health 6:602–609
Berlin M, Ullberg S (1963b) Accumulation and retention of mercury in the mouse. III. An autoradiographic comparison of methyl mercuric dicyandiamide with inorganic mercury. Arch Environ Health 6:610–616
Burton GV, Meikle AW (1980) Acute and chronic methyl mercury poisoning impairs rat adrenal and testicular function. J Toxicol Environ Health 6:597–606
Chang LW (1977) Neurotoxic effects of mercury — a review. Environ Res 14:329–373
Clarkson TW (1972) The pharmacology of mercury compounds. Ann Rev Pharmacol 12:375–406
Clarkson TW, Hamada R, Amin-Zachi L (1984) Mercury. In: Changing metal cycles and human health. Dahlem Konferenzen 1984. Springer, Berlin Heidelberg New York Tokyo, pp 285–309
Danscher G (1981a) Localization of gold in biological tissue. A photochemical method for light and electron microscopy. Histochemistry 71:81–88
Danscher G (1981b) Light and electron microscopic localization of silver in biological tissue. Histochemistry 71:177–186
Danscher G, Møller-Madsen B (1985) Silver amplification of mercury sulphide and selenide. A histochemical method for light and electron microscopic localization of mercury in tissue. Histochem Cytochem 33 (3): 219–228
Dingemans KP, Feltkamp CA (1972) Nongranulated cells in the mouse adenohypophysis. Z Zeilforsch 124:387–405
Doi R, Tagawa M (1983) A study on the biochemical and biological behaviour of methylmercury. Toxicol Appl Pharmacol 69:407–416
Elhassani SB (1983) The many faces of methylmercury poisoning. J Toxicol Clin Toxicol 19:875–906
Farquhar MG (1978) Recovery of surface membrane in the anterior pituitary cells. J Cell Biol 77:(35–42)R
Farquhar MG, Shutelsky EH, Hopkins CR (1975) Structure and function of the anterior pituitary and dispersed pituitary cells. In vitro studies. In: Tixier-Vidal A, Farquhar MG (eds) The anterior pituitary. Academic Press, New York, pp 83–135
Fimreite N (1970) Mercury uses in Canada and their possible hazards as source of mercury contamination. Environ Pollut 1:119–131
Fimreite N, Karstad L (1971) Effects of dietary methyl mercury on red-tailed hawks. J Wildl Mgmt 30:293–300
Fruton JS, Mycek MJ (1956) Studies on beef spleen cathepsin C1. Arch Biochem Biophys 65:11–20
Fukuda T (1973) Agranular stellate cells (so called follicular cells) in human fetal and adult adenohypophysis and pituitary adenoma. Virchows Arch [Pathol Anat] 359:19–30
Hughes WL (1957) A physicochemical rationale for the biological activity of mercury and its compounds. Ann NY Acad Sci (USA) 65:454–460
Hunter D, Russel D (1954) Focal cerebral and cerebellar atrophy in a human subject due to organic mercury compounds. J Neurol Neurosurg Psychiat 17:235–241
Kurland LT, Faro S, Siedler H (1960) Minamata disease. The outbreak of a neurologic disorder in Minamata, Japan and its relationship to the ingestion of seafood contaminated by mercuric compounds. World Neurol 1:370–395
Liwska J (1978) Investigation of ultrastructure of the adenohypophysis in the domestic pig (Susserota domestica). Part II: “Dark cells” in the pars anterior. Folia Histochem Cytochem 16:315–322
Lorenson MY, Jacobs LS (1984) Depletion of bovine pituitary prolactin by cysteamine involves a thiol: disulfide mechanism. Endocrinology 115:1492–1495
McDonald JK, Callahan PX, Ellis S, Smith RE (1971) Polypeptide degradation by dipeptidyl aminopeptidase I (cathepsin C) and related peptidases. In: Barrett AJ, Dingle JT (eds) Tissue proteinases. North Holland, Amsterdam, pp 69–107
Nordberg GF, Skerfving S (1972) Metabolism. In: Friberg L, Vostal F (eds) Mercury in the environment. CRC Press, Cleveland, Ohio, pp 29–91
Norseth T, Brendeford M (1971) Intracellular distribution of inorganic and organic mercury in rat liver after exposure to methylmercury salts. Biochem Pharmacol 20:1101–1107
Rosenzweig LJ, Farquhar MG (1980) Sites of sulfate incorporation into mammotrophs and somatotrophs of the rat pituitary as determined by quantitative electron microscopic audioradiography. Endocrinology 107:422–431
Rosenzweig LF, Kanwar YS (1984) Quantitative determination of the intracellular fate of internalized plasma membrane in dissociated pituitary prolactin cells utilizing a radioiodinated cationic ferritin probe (CFI) and electron microscopic autoradiography. Am J Anat 169:193–206
Silberberg I (1968) Percutaneous absorption of mercury in man. I. A study by electron microscopy of the passage of topically applied mercury salts through stratum-corneum of subjects not sensitive to mercury. J Invest Dermatol 50:323–331
Simpson RB (1961) Association constants of methylmercury with sulphydryl and other bases. J Am Chem Soc 83:4711–4717
Slaby F, Farquhar MG (1980) Characterization of rat somatotroph and mammotroph secretory granules: Presence of sulfated macromolecules. Mol Cell Endocrinol 18:33–40
Stoewsand GS, Anderson JL, Guthenmann WH, Bache CA, Lisk DJ (1971) Eggshell thinning in Japanese quail fed mercuric chloride. Science 173:1030–1031
Suter KE (1975) Studies on the dominant-lethal and fertility effects of heavy metal compounds methylmercuric hydroxide, mercuric chloride and cadmium chloride in male and female mice. Mutant Res 30:365–374
Takeuchi T (1968) Pathology of Minamata diseases. In: Minamata disease (Organic Mercury Poisoning). Study Group of Minamata disease. Kumamoto University, Japan
Thorlacius-Ussing O, Møller-Madsen B, Danscher G (1985) Intracellular accumulation of mercury in the anterior pituitary of rats exposed to mercuric chloride. Exp Mol Pathol 42:278–286
Timm F (1958) Zur Histochemie der Schwermetalle. Das Sulfid-Silber-Verfahren. Dtsch Z Ges Gerichtl Med 46:706–711
Yamashita K (1969) Electron microscopic observations on the postnatal development of the anterior pituitary of the mouse. In: GUMMA Symposia on endocrinology 6:177–194
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Møller-Madsen, B., Thorlacius-Ussing, O. Accumulation of mercury in the anterior pituitary of rats following oral or intraperitoneal administration of methyl mercury. Virchows Archiv B Cell Pathol 51, 303–311 (1986). https://doi.org/10.1007/BF02899039
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DOI: https://doi.org/10.1007/BF02899039