We studied the effects of course (10 days) intraperitoneal injections of salts of gold and silver and colloid solutions of nanoparticles of these metals on the size (mean value of the cross-sections) of the nuclei of hypothalamic neurocytes localized in the preoptic and arcuate nuclei of immature male albino rats. In animals injected with a solution containing tetrachloroaurate ions, the examined morphometric parameters in both structures significantly exceeded the control values. Injections of gold nanoparticles led to significant decreases in the cross-sections of the neurocyte nuclei; thus, the pattern of the effects of gold depended on its physicochemical form. Injections of both silver salt (AgNO3) and silver nanoparticles led to drops in the mean values of cross-sections of the nuclei of neurocytes. Therefore, shifts of the studied morphofunctional characteristics of the cells belonging to the hypothalamic nuclei resulting from systemic injections of colloid solutions (nanoparticles) of gold and silver are indicative of suppression of functions of the central link of the hypothalamo-hypophyseal-gonadal system after the influence of these factors.
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References
T. M. Boichouk, N. I. Andriichouk, and L. I. Vlasyuk, “On the problem of estimating the toxicity of silver nanoparticles,” Klin. Éksp. Patol., 11, No. 4, 151-157 (2012).
L. Yildirimer, N. T. K. Thanh, M. Loizidou, et al., “Toxicology and clinical potential of nanoparticles,” Nano Today, 6, No. 6, 585-607 (2011).
X. Lu, Y. Liu, X. Kong, et al., “Nanotoxicity: a growing need for study in the endocrine system,” Small, 9, Nos. 9/10, 1654-1671 (2013).
A. M. Monaco and M. Giugliano, “Carbon-based smart nanomaterials in biomedicine and neuroengineering,” Beilstein J. Nanotechnol., 5, 1849-1863 (2014).
S. Das, J. M. Dowding, K. E. Klump, et al., “Cerium oxide nanoparticles: applications and prospects in nanomedicine,” Nanomedicine (Lond.), 8, No. 9, 1483-1508 (2013).
C. A. Dos Santos, M. M. Seckler, A. P. Ingle, et al., “Silver nanoparticles: therapeutical uses, toxicity, and safety issues,” J. Pharm. Sci., 103, No. 7, 1931-1944 (2014).
E. C. Dreaden, A. M. Alkiany, X. Huang, et al., “The golden age: gold nanoparticles for biomedicine,” Chem. Soc. Rev. 41, No. 7, 2740-2779 (2011).
M. Eghtedari, A. V. Liopo, J. A. Copland, et al., “Engineering of hetero-functional gold nanorods for the in vivo molecular targeting of breast cancer cells,” Nano Lett. 9, No. 1, 287-291 (2009).
A. M. Pekkanen, M. R. DeWitt, and M. N. Rylander, “Nanoparticle enhanced optical imaging and phototherapy of cancer,” J. Biomed. Nanotechnol., 10, No. 9, 1677-1712 (2014).
K. Bhattacharyya, B. S. Goldschmidt, M. Hannink, et al.,”Gold nanoparticle-mediated detection of circulating cancer cells,” Clin. Lab. Med. 32, No. 1, 89-101 (2012).
P. C. Ray, H. Yu, and P. P. Fu, “Nanogold-based sensing of environmental toxins: excitement and challenges,” J. Environ. Sci. Health C Environ. Carcinog. Ecotoxicol. Rev., 29, No. 1, 52-89 (2011).
M. Mishra and P. Chauhan, “Nanosilver and its medical implications,” J. Nanomed. Res., 2, No. 5, 20-30 (2015).
J. H. Lee, Y. S. Kim, K. S. Song, et al., “Biopersistence of silver nanoparticles in tissues from Sprague–Dawley rats,” Part. Fibre Toxicol., 10, No. 1, 36 (2013).
M. Yamada, M. Foote, and T.W. Prow, “Therapeutic gold, silver, and platinum nanoparticles,” Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol., 7, No. 3, 428-445 (2015).
L. Rizzello and P. P. Pompa, “Nanosilver-based antibacterial drugs and devices: mechanisms, methodological drawbacks, and guidelines,” Chem. Soc. Rev., 43, No. 5, 1501-1518 (2014).
X. Liu, P. Y. Lee, C. M. Ho, et al., “Silver nanoparticles mediate differential responses in keratinocytes and fibroblasts during skin wound healing,” Chem. Med. Chem., 5, No. 3, 468-475 (2010).
M. Thakur, H. Gupta, D. Singh, et al., “Histopathological and ultra-structural effects of nanoparticles on rat testis following 90 days (chronic study) of repeated oral administration,” J. Nanobiotechnol., 12, No. 1, 42 (2014).
S. T. Zakhidov, S. M. Pavlyuchenkova, T. L. Marshak, et al., “Effect of gold nanoparticles on mouse spermatogenesis,” Biol. Bull., 39, No. 3, 229-236 (2012).
M. G. Matvienko, A. S. Pustovalov, N. A. Buzynskaya, et al., “Morphofunctional modifications of cells of the preoptic hypothalamic nucleus of prepubescent rats under conditions of stimulation and blocking of the α-adrenergic and kisspeptinergic systems,” Neurophysiology, 45, Nos. 5/6, 417-422 (2013).
M. G. Matvienko, A. S. Pustovalov, and N. E. Dzerzhinsky, “Variety of functions and effects of kisspeptin,” Biopolym. Cell, 29, No. 1, 11-20 (2013).
H. M. Dungan, D. K. Clifton and R. A. Steiner, “Minireview: kisspeptin neurons as central processors in the regulation of gonadotropin-releasing hormone secretion,” Endocrinology, 147, No. 3, 1154-1158 (2006).
J. S. Fuqua, “Treatment and outcomes of precocious puberty: an update,” J. Clin. Endocrinol. Metab., 98, No. 6, 2198-2207 (2013).
V. M. Navarro and M. Tena-Sempere, “Neuroendocrine control by kisspeptins: Role in metabolic regulation of fertility,” Nat. Rev. Endocrinol., 8, No. 1, 40-53 (2011).
R. D. Lillie, Histophatologic Technique and Practical Histochemistry [Russian translation], Mir, Moscow (1969).
N. Biswas, A. Chattopadhyay, and M. Sarkar, “Effects of gold on testicular steroidogenic and gametogenic functions in immature male albino rats,” Life Sci., 76, No. 6, 629-636 (2004).
E. Frohlich, “Cellular targets and mechanisms in the cytotoxic action of non-biodegradable engineered nanoparticles,” Curr. Drug Metab., 14, No. 9, 976-988 (2013).
W. H. De Jong, W. I. Hagens, P. Krystek, et al., “Particle size-dependent organ distribution of gold nanoparticles after intravenous administration,” Biomaterials, 29, No. 12, 1912-1919 (2008).
A. Pizent, B. Tariba, and T. Živković, “Reproductive toxicity of metals in men,” Arh. Hig. Rada Toksikol., 63, Suppl. 1, 35-46 (2012).
B. Huppertz, “Nanoparticles: Barrier thickness matters,” Nat. Nanotechnol., 6, No. 12, 758-759 (2012).
O. Gordon, T. Vig Slenters, P.S. Brunetto, et al., “Silver coordination polymers for prevention of implant infection: thiol interaction, impact on respiratory chain enzymes, and hydroxyl radical induction,” Antimicrob. Agents Chemother., 54, No. 10, 4208-4218 (2010).
J. Y. Kim, C. Lee, M. Cho, et al., “Enhanced inactivation of E. coli and MS-2 phage by silver ions combined with UV-A and visible light irradiation,” Water Res. 42, Nos. 1/2, 356-362 (2009).
W. Likus, G. Bajor, and K. Siemianowicz, “Nanosilver – does it have only one face?” Acta Biochim. Pol., 60, No. 4, 495-501 (2013).
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Kalynovskyi, V.Y., Pustovalov, A.S., Grodzyuk, G.Y. et al. Effects of Systemic Introductions of Nanoparticles and Salts of Gold and Silver on the Size of the Nuclei of Hypothalamic Neurons in Male Rats. Neurophysiology 48, 259–263 (2016). https://doi.org/10.1007/s11062-016-9597-3
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DOI: https://doi.org/10.1007/s11062-016-9597-3