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Localization of gold in biological tissue

A photochemical method for light and electronmicroscopy

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Summary

A detailed description is given of a method by which gold can be visualized in frozen, paraffin and Epon sections. Histological sections from animals treated with gold compounds are exposed to UV-light from 30 min to several hours. The reduced, metallic gold is then visualized by means of a photographic developer containing silver lactate. Light- and electronmicroscope photographs showing gold in different organs from rats and mice treated with aurothioglucose, aurothiosulfate and aurothiomalate are presented.

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References

  • Blackstad TW (1975) Electron microscopy of experimental axonal degeneration in photochemically modified Golgi preparations: A procedure for precise mapping of nervous connections. Brain Res 95:191–210

    Google Scholar 

  • Danscher G (1980) Histochemical demonstration of heavy metals. A revised version of the sulphide silver method suitable for both light and electronmicroscopy. (In preparation)

  • Danscher G, Hansen HJ (1980) X-ray microprobe analysis of tissues from rats and mice intravitally treated with gold and silver. (In preparation)

  • Doré JL (1974) The demonstration and distribution of gold in tissue sections. Thesis (London)

  • Doré JL, Vernon-Roberts BA (1976) A method for the selective demonstration of gold in tissue sections. Med Lab Sci 33:209–213

    Google Scholar 

  • Gilg E (1952) A photochemical method for microdetection of gold in tissue sections. Acta Psychiatr Scand 27:43–56

    Google Scholar 

  • Gottlieb LN, Smith PM, Smith EM (1972) Tissue gold concentration in a rheumatoid arthristic receiving chrysotherapy. Arthritis and Rheumatism 15:16–22

    Google Scholar 

  • Jessop JD, Vernon-Roberts B, Harris J (1973) Effects of gold salts and prednisolone on inflammatory cells. Ann Rheum Dis 32:294–300

    Google Scholar 

  • Liesegang RE, Rieder W (1921) Versuche mit einer “Keimmethode” zum Nachweis von Silber in Gewebsschnitten. Z Wiss Mikrosk 38:334–338

    Google Scholar 

  • Persillin RH, Ziff M (1966) The effect of gold salt on lysosomal enzymes of the peritoneal macrophage. Arthritis and Rheumatism 9:57–65

    Google Scholar 

  • Pillai CKS, Nandi US (1973) Binding of gold (III) with DNA. Biopolymers 12:1431–1435

    Google Scholar 

  • Puddephatt RS (1978) The chemistry of gold. Top Org Gen Chem [NY] 16:160

    Google Scholar 

  • Querido A (1947) Gold intoxication of nervous elements. On the permeability of the blood-brain-barrier. Acta Psychiatr 12:151

    Google Scholar 

  • Roberts WJ (1935) A new procedure for the detection of gold in animal tissues. Proc R Acad [Amsterdam] 38:540–544

    Google Scholar 

  • Vernon-Roberts B, Doré JL, Jessop JD, Henderson WJ (1976) Selective concentration and localization of gold in macrophages of synovial and other tissues during and after chrysotherapy in rheumatoid patients. Ann Rheum Dis 35:477–486

    Google Scholar 

Download references

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Authors and Affiliations

  1. Institute of Anatomy B, University of Aarhus, DK-8000, Aarhus C, Denmark

    G. Danscher

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  1. G. Danscher
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Danscher, G. Localization of gold in biological tissue. Histochemistry 71, 81–88 (1981). https://doi.org/10.1007/BF00592572

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  • DOI: https://doi.org/10.1007/BF00592572

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