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Species relationships between a wild tetraploid potato species,Solanum acaule bitter, and its related species as revealed by rflps of chloroplast and nuclear DNA

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Abstract

Solanum acaule Bitter is a wild, disomic tetraploid potato species widely distributed in the South America. In this study, species relationships between eight accessions ofS. acaule and 108 accessions of 27 morphologically closely related species were investigated using chloroplast and nuclear DNA RFLPs. Chloroplast DNA types were determined for most of the accessions based on restriction digestion patterns of entire chloroplast DNA. C type chloroplast DNA was common inS. acaule andS. albicans (a hexaploid derivative fromS. acaule), which was also found inS. megistacrolobum, S. sanctae-rosae,S. raphanifolium, and several other Peruvian species in seriesTuberosa. Nuclear DNA was analyzed by Southern hybridization with 31 single-copy RFLP probes. One hundred and thirty-four bands were scored, from which a UPGMA dendrogram was constructed to show overall similarities among accessions.Solanum acaule andS. albicans (andS. demissum) formed a cluster with a high similarity, and then, this group was clustered withS. megistacrolobum andS. sanctae-rosae. The resulting cluster was well separated from other clusters. Thus, we suggest that an initial tetrasomic tetraploid arose from eitherS. megistacrolobum orS. sanctae-rosae. Interestingly, a Mexican hexaploid speciesS. demissum showed the closest similarity toS. acaule andS. albicans, suggesting that they shared a common ancestry.

Resumen

La papaSolanum acaule amarga es una especie silvestre tetraploide disómica ampliamente distribuida en América del Sur. En este estudio se investigó el parentesco entre 8 accesiones de S.acaule y 108 accesiones de 27 especies emparentadas morfológicamente de forma cercana, para lo cual se usó el procedimiento de RFLP (polimorfismo en la longitud de los fragmentos de restricción) del ADN del cloroplasto y del núcleo. Los tipos de ADN del cloroplasto fueron determinados por el mayor número de accesiones basadas en los patrones de restricción de la digestión del ADN total del cloroplasto. El tipo C de cloroplasto fue común enS. Acaule yS. albicans (un hexaploide derivado deS. Acaule) el cual también fue encontrado enS. megistacrolobum, S. sanctae-rosae, S. raphanifolium y en muchas otras especies peruanas de la serieTuberosa. El ADN del núcleo fue analizado por hibridación del Sur con 31 pruebas de RFLP en copias individuales. Se contabilizaron ciento treinta y cuatro bandas, con las cuales se construyó un dendograma UPGMA para mostrar todas las similitudes entre accesiones.Solanum acaule yS. albicans (y tambiénS. demissum) formaron un grupo con una alta similitud y, por consiguiente, fueron agrupados con accesiones deS. megistacrolobum y S. sanctae-rosae. El grupo resultante fue bien separado de otros. Por lo tanto, sugerimos que se produjo un tetraploide tetrasómico inicial ya sea deS. megistacrolobum o deS. sanctae-rosae. De manera interesante, una especie hexaploide mexicana,S. demissun, mostró la muy cercana similitud conS. acaule yS. albicans, lo cual sugiere que comparten un ancestro común.

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  1. Experimental Farm, Kobe University, 1348 Uzurano, 675-2103, Kasai, Hyogo, Japan

    Kumi Nakagawa & Kazuyoshi Hosaka

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  1. Kumi Nakagawa
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Nakagawa, K., Hosaka, K. Species relationships between a wild tetraploid potato species,Solanum acaule bitter, and its related species as revealed by rflps of chloroplast and nuclear DNA. Am. J. Pot Res 79, 85–98 (2002). https://doi.org/10.1007/BF02881517

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