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A rapid and simple method for determination of potato chloroplast DNA type

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Abstract

A procedure for restriction enzyme analysis of the potato chloroplast DNA (ctDNA) is described. The advantages of this method are: 1) rapid determination of ctDNA type, 2) no ultracentrifugation, and 3) low cost of analyses. This method makes it easy to distinguish the ctDNA types of wild and cultivated potato accessions.

Compendio

Se describe un procedimiento para el análisis de la enzima de restricción del DNA (ctDNA) en los cloroplastos de papa. Las ventajas de este método son: (1) la rápida determinación del tipo de ctDNA, (2) el no requerir ultracentrifugación, y (3) el bajo costo del análisis. Este método permite distinguir fácilmente los tipos de ctDNA en las entradas de papas silvestres y cultivadas.

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Author information

Authors and Affiliations

  1. Department of Horticulture, University of Wisconsin, 53706, Madison, WI

    Kazuyoshi Hosaka (Postdoctoral Fellow)

  2. USDA, Agricultural Research Service, Department of Horticulture, University of Wisconsin, 53706, Madison, WI

    Robert E. Hanneman (Research Geneticist)

Authors
  1. Kazuyoshi Hosaka
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  2. Robert E. Hanneman
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Additional information

This work is a cooperative investigation of the U.S. Department of Agriculture, Agricultural Research Service and the Wisconsin Agricultural Experiment Station.

Reference to a specific brand or firm name does not constitute endorsement by the U.S. Department of Agriculture over others of a similar nature not mentioned.

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Hosaka, K., Hanneman, R.E. A rapid and simple method for determination of potato chloroplast DNA type. American Potato Journal 64, 345–353 (1987). https://doi.org/10.1007/BF02853596

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  • DOI: https://doi.org/10.1007/BF02853596

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