Skip to main content
SpringerLink
Log in

Collapse of species boundaries in the wild potatoSolanum brevicaule complex (Solanaceae, S. sect.Petota): Molecular data

  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

TheSolanum brevicaule complex is a group of morphologically very similar wild and cultivated potato taxa (Solanum sect.Petota). This study uses single to low-copy nuclear RFLPs and RAPDs to investigate their species boundaries and relationships. Cladistic analyses of both data sets are largely concordant with each other and with a recently published phenetic analyses of the same accessions using morphology. All three data sets separate members of the complex into populations from Peru and immediately adjacent northwestern Bolivia, including most cultivated species accessions, and populations from northwestern Bolivia to Argentina. The molecular results suggest that the complex is paraphyletic as currently circumscribed. Many species of theS. brevicaule complex should be relegated to synonymy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aldrich, P. R., Doebley, J., 1992: Restriction fragment variation in the nuclear and chloroplast genomes of cultivated and wildSorghum bicolor. — Theor. Appl. Genet.85: 293–302.

    Google Scholar 

  • Astley, D., Hawkes, J. G., 1979: The nature of the Bolivian weed speciesSolanum sucrense Hawkes. — Euphytica28: 685–696.

    Google Scholar 

  • Bamberg, J. B., Martin, M. W., Schartner, J. J., Spooner, D. M., 1996: Inventory of tuber-bearingSolanum species. — Sturgeon Bay, Wisconsin: Potato Introduction Station, NRSP-6.

    Google Scholar 

  • Bark, O. H., Havey, M. J., 1995: Similarities and relationships among populations of the bulb onion as estimated by nuclear RFLPs. — Theor. Appl. Genet.90: 407–414.

    Google Scholar 

  • Baum, D. A., Donoghue, M. J., 1995: Choosing among alternative “phylogenetic” species concepts. — Syst. Bot.20: 560–573.

    Google Scholar 

  • Bonierbale, M. W., Ganal, M. W., Tanksley, S. D., 1995: Applications of restriction fragment length polymorphisms and genetic mapping in potato breeding and molecular genetics. — InVayda, M. E., Park, W. D., (Eds): The molecular and cellular biology of the potato, pp. 13–24. — Wallingford, UK: CAB International.

    Google Scholar 

  • Brubaker, C. L., Wendel, J. F., 1994: Reevaluating the origin of domesticated cotton (Gossypium hirsutum;Malvaceae) using nuclear restriction fragment length poly-morphisms (RFLPs). — Amer. J. Bot.81: 1309–1326.

    Google Scholar 

  • Brucher, H., 1964: El origin de la papa (Solanum tuberosum) — Physis24: 439–452.

    Google Scholar 

  • Brummer, E. C., Bouton, J. H., Kochert, G., 1995: Analysis of annualMedicago species using RAPD markers. — Genome38: 362–367.

    Google Scholar 

  • Bruneau, A. E., Dickson, E., Knapp, S., 1995: Congruence of chloroplast DNA restriction site characters with morphological and isozyme data inSolanum sect.Lasiocarpa. — Canad. J. Bot.73: 1151–1167.

    Google Scholar 

  • Catalán, P., 1995: Molecular phylogeny of the grass genusBrachypodium P. Beauv. based of RFLP and RAPD analysis. — Bot. J. Linn. Soc.117: 263–280.

    Google Scholar 

  • Cribb, P. J., Hawkes, J. G., 1986: Experimental evidence for the origin ofSolanum tuberosum subspeciesandigena. — InD'Arcy, W. G., (Ed.):Solanaceae: biology and systematics, pp. 383–404. — New York: Columbia University Press.

    Google Scholar 

  • Dawson, I. K., Simons, A. J., Waugh, R., Powell, W., 1996: Detection and pattern of interspecific hybridization betweenGliricidia sepium andG. maculata in Meso-America revealed by PCR-based assays. — Molec. Ecol.5: 89–98.

    Google Scholar 

  • Debener, T., Salamini, F., Gebhardt, C., 1990: Phylogeny of wild and cultivatedSolanum species based on nuclear restriction fragment length polymorphism (RFLPs). — Theor. Appl. Genet.79: 360–368.

    Google Scholar 

  • Dos Santos, J. B., Nienhuis, J., Skroch, P., Tivang, J., Slocum, M. K., 1994: Comparison of RAPD and RFLP genetic markers in determining genetic similarities amongBrassica oleracea L. genotypes. — Theor. Appl. Genet.87: 909–915.

    Google Scholar 

  • Doyle, J. J., 1995: The irrelevance of allele tree topologies for species delimitation, and a non-topological alternative. — Syst. Bot.20: 574–588.

    Google Scholar 

  • Feinberg, A. P., Volgelstein, B., 1984: Addendum. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. — Analyt. Biochem.137: 266–267.

    Google Scholar 

  • Giannattasio, R. B., Spooner, D. M., 1994: A reexamination of species boundaries and hypotheses of hybridization concerningSolanum megistacrolobum andS. toralapanum (Solanum sect.Petota, seriesMegistacroloba): molecular data. — Syst. Bot.19: 106–115.

    Google Scholar 

  • Halward, T., Stalker, T., Larue, E., Kochert, G., 1992: Use of single-primer DNA amplifications in genetic studies of peanut (Arachis hypogaea L.). — Pl. Molec. Biol.18: 315–325.

    Google Scholar 

  • Hanneman, R. E., Jr., 1989: The potato germplasm resource. — Amer. Potato J.66: 655–667.

    Google Scholar 

  • Hawkes, J. G., 1958: Kartoffel: I. Taxonomy, cytology and crossability. — InKappert, H., Rudorf, W., (Eds): Handbuch der Pflanzenzüchtung,3, pp. 1–43. 2nd edn. — Berlin: Parey.

    Google Scholar 

  • —, 1990: The potato: evolution, biodiversity and genetic resources. — Washington, D. C.: Smithsonian Institution Press.

    Google Scholar 

  • —, 1969: The potatoes of Argentina, Brazil, Paraguay and Uruguay: a biosystematic study. — Oxford: Oxford University Press.

    Google Scholar 

  • —, 1989: The potatoes of Bolivia: their breeding value and evolutionary relationships. — Oxford: Oxford University Press.

    Google Scholar 

  • Heun, M., Murphy, J. P., Phillips, T. D., 1994: A comparison of RAPD and isozyme analyses for determining the genetic relationships amongAvena sterilis L. accessions. — Theor. Appl. Genet.87: 689–696.

    Google Scholar 

  • Hilu, K. W., Stalker, H. T., 1995: Genetic relationships between peanut and wild species ofArachis sect.Arachis (Fabaceae): evidence from RAPDs. — Pl. Syst. Evol.198: 167–178.

    Google Scholar 

  • Hosaka, K., 1995: Successive domestication and evolution of the Andean potatoes as revealed by chloroplast DNA restriction endonuclease analysis. — Theor. Appl. Genet.90: 356–363.

    Google Scholar 

  • —,Hanneman, R. E., Jr., 1988: Origin of chloroplast DNA diversity in Andean potatoes. — Theor. Appl. Genet.76: 333–340.

    Google Scholar 

  • —, 1992: RFLP analysis of the wild potato species,Solanum acaule Bitter (Solanum sect.Petota). — Theor. Appl. Genet.84: 851–858.

    Google Scholar 

  • Kardolus, J. P., Van Eck, H. J., Van Den Berg, R. G., 1997: The potential of AFLPs in biosytematics: a first application inSolanum taxonomy. — Pl. Syst. Evol.210: 87–103.

    Google Scholar 

  • Kim, K. J., Jansen, R. K., 1994: Comparisons of phylogenetic hypotheses among different data sets in dwarf dandelions (Krigia, Asteraceae); additional information from internal transcribed spacer sequence of nuclear ribosomal DNA. — Pl. Syst. Evol.190: 157–185.

    Google Scholar 

  • Loarce, Y., Gallego, R., Ferrer, E., 1996: A comparative analysis of the genetic relation-ships between rye cultivars using RFLP and RAPD markers. — Euphytica88: 107–115.

    Google Scholar 

  • Luckow, M., 1995: Species concepts: assumptions, methods, and applications. — Syst. Bot.20: 589–605.

    Google Scholar 

  • Maddison, W., 1995: Phylogenetic histories within and among species. — InHoch, P. C., Stephenson, A. G., (Eds): Experimental and molecular approaches to plant biosystematics, pp. 273–287. — St. Louis: Missouri Botanical Garden

    Google Scholar 

  • Margale, E., Herve, Y., Hu, J., Ouiros, C. F., 1995: Determination of genetic variability by RAPD markers in cauliflower, cabbage and kale local cultivars from France. — Genet. Res. Crop Evol.42: 281–289.

    Google Scholar 

  • Matsubayashi, M., 1981: Phylogenetic relationships in the potato and its related species. — InTsuchiya, T., Gupta, P. K., (Eds): Chromosome engineering in plants: genetic, breeding, evolution, part B, pp. 93–118. — Amsterdam: Elsevier.

    Google Scholar 

  • Mcdade, L. A., 1992: Hybrids and phylogenetics systematics II. The impact of hybrids on cladistic analysis. — Evolution46: 1329–1346.

    Google Scholar 

  • —, 1995: Hybridization and phylogenetics. — InHoch, P. C., Stephenson, A. G., (Eds): Experimental and molecular approaches to plant systematics, pp. 305–331. — St. Louis: Missouri Botanical Garden.

    Google Scholar 

  • Mickevich, M. F., Farris, J. S., 1981: The implication of congruence inMenidia. — Syst. Zool.30: 351–370.

    Google Scholar 

  • Miller, J. T., 1997: Collapse of species in the wild potatoSolanum brevicaule complex (Solanaceae: sect.Petota). — Ph.D Thesis, University of Wisconsin-Madison.

  • —, 1996: Introgression ofSolanum chacoense (Solanum sect.Petota) upland populations reexamined. — Syst. Bot.21: 461–475.

    Google Scholar 

  • Nixon, K. C., Wheeler, O. D., 1990: An amplification of the phylogenetic species concept. — Cladistics6: 211–223.

    Google Scholar 

  • Ochoa, C. M., 1990: The potatoes of South American: Bolivia. — Cambridge: Cambridge University Press.

    Google Scholar 

  • Olmstead, R. G., 1995: Species concepts and plesiomorphic species. — Syst. Bot.20: 623–630.

    Google Scholar 

  • —, 1994: Chloroplast DNA and systematics: a review of methods and data analysis. — Amer. J. Bot.81: 1205–1224.

    Google Scholar 

  • —, 1994: Combining data in phylogenetic systematics: an empirical approach using three molecular data sets in theSolanaceae. — Syst. Biol.43: 467–481.

    Google Scholar 

  • Oxelman, B., 1996: RAPD patterns, nrDNA ITS sequences and morphological patterns inSilene sectionSedoideae (Caryophyllaceae). — Pl. Syst. Evol.201: 93–116.

    Google Scholar 

  • Prince, J. P., Lackney, V. K., Angeles, C., Blauth, J. R., Kyle, M. M., 1995: A survey of DNA polymorphism within the genusCapsicum and the fingerprinting of pepper cultivars. — Genome38: 224–231.

    Google Scholar 

  • Rice, K. A., Donoghue, M. J., Olmstead, R. G., 1997: Reanalysis of a largerbcL dataset: implications for future phylogenetic studies. — Syst. Biol.46: 554–562.

    Google Scholar 

  • Rieseberg, L. H., 1996: Homology among RAPD fragments in interspecific comparisons. — Molec. Evol.5: 99–105.

    Google Scholar 

  • —, 1994: Are many plant species paraphyletic? — Taxon43: 21–32.

    Google Scholar 

  • Sas, 1995: JMP statistics made visual, version 3.02. — Cary, North Carolina: SAS Institute, Inc.

    Google Scholar 

  • Skroch, P. W., Nienhuis, J., 1995: Qualitative and quantitative characterization of RAPD variation among snap bean (Phaseolus vulgaris) genotypes. — Theor. Appl. Genet.91: 1078–1085.

    Google Scholar 

  • Shanmukhaswami, S. S., De Oliveira, A. C., Godwin, I. D., Bennetzen, J. L., 1995: Assessment of genome origins and genetic diversity in the genusEleusine with DNA markers. — Genome38: 757–763.

    Google Scholar 

  • Smith, J. J., Scott-Craig, J. S., Leadbetter, J. R., Bush, G. L., Roberts, D. L., Fulbright, D. W., 1994: Characterization of random amplified polymorphic DNA (RAPD) products fromXanthomonas campestris and some comments on the use of RAPD products in phylogenetic analyses. — Molec. Phylogenet. Evol.3: 135–145.

    Google Scholar 

  • Song, K., Osborn, T. C., Williams, P. H., 1990:Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs) 3. Genome relationships inBrassica and related genera and the origin ofB. oleracea andB. rapa (syn.campestris). — Theor. Appl. Genet.79: 497–506.

    Google Scholar 

  • Spooner, D. M., Castillo-T., R., 1997: Reexamination of series relationships of South American wild potatoes (Solanaceae:Solanum Sect.Petota): evidence from chloroplast DNA restriction site variation. — Amer. J. Bot.84: 671–685.

    Google Scholar 

  • —, 1992: Reexamination of series relationships of Mexican and Central American wild potatoes (Solanum sect.Petota): evidence from chloroplast DNA restriction site variation. — Syst. Bot.17: 432–448.

    Google Scholar 

  • —, 1996: Comparison of four molecular markers in measuring relationships among the wild potato relativesSolanum sectionEtuberosum (subgenusPotatoe). — Theor. Appl. Genet.92: 532–540.

    Google Scholar 

  • —, 1992: An analysis of recent taxonomic concepts in wild potatoes (Solanum sect.Petota). — Genet. Res. Crop Evol.39: 23–37.

    Google Scholar 

  • —, 1997: Species boundaries and interrelationships of two closely related sympatric diploid wild potato species,Solanum astleyi andS. boliviense based on RAPD's. — Theor. Appl. Genet.95: 764–771.

    Google Scholar 

  • Stammers, M., Harris, J., Evans, G. M., Hayward, M. D., Forster, J. W., 1995: Use of random PCR (RAPD) technology to analyze phylogenetic relationships in theLolium/Festuca complex. — Heredity74: 19–27.

    Google Scholar 

  • Swofford, D. L., 1991: When are phylogeny estimates from morphological and molecular data incongruent? — InMiyamoto, M. M., Cracraft, J., (Eds): Phylogenetic analysis of DNA sequences, pp. 295–333. — New York: Oxford University Press.

    Google Scholar 

  • —, 1993: PAUP: phylogenetic analyses using parsimony, version 3.1.1. Computer program. — Champaign, IL.: Illinois Natural History Survey.

    Google Scholar 

  • Tanksley, S. D., Ganal, M. W., Prince, J. P., De Vicente, N. C., Bonierbale, M. W., Broun, P., Fulton, T. M., Giovannoni, J. J., Grandillo, S., Martin, G. B., Messenguer, R., Miller, J. C., Miller, L., Paterson, A. H., Pineda, O., Roder, M. S., Wing, R. A., Wu, W., Young, N. D., 1992: High density molecular linkage maps of the tomato and potato genomes. — Genetics132: 1141–1160.

    Google Scholar 

  • Thormann, C. E., Ferreira, M. E., Camargo, L. E. A., Tivang, J. G., Osborn, T. C., 1994: Comparison of RFLP and RAPD markers for estimating genetic relationship within and among cruciferous species. — Theor. Appl. Genet.88: 973–980.

    Google Scholar 

  • Ugent, D., 1966: Hybrid weed complexes inSolanum, sectionTuberarium. — Ph.D. Dissertation, University of Wisconsin, Madison, Wisconsin.

    Google Scholar 

  • —, 1970: The potato. — Science170: 1161–1166.

    Google Scholar 

  • Van Coppenolle, B., Watanabe, I., Van Hove, C., Mccouch, S. R., 1993: Genetic diversity and phylogeny analysis ofAzolla based on DNA amplification by arbitrary primers. — Genome36: 686–693.

    Google Scholar 

  • Van Buren, R., Harper, K. T., Andersen, W. R., Stanten, D. J., Seymoum, S., England, J. L., 1994: Evaluating the relationship of autumn buttercup (Ranunculus acriformis var.aestivalis) to some close congeners using random amplified polymorphic DNA. — Amer. J. Bot.81: 514–519.

    Google Scholar 

  • Van Den Berg, R. G., Groendijk-Wilders, N., Kardolas, J. P., 1996: The wild ancestors of cultivated potato: thebrevicaule-complex. — Acta Bot. Neerl.45: 157–171.

    Google Scholar 

  • —, 1998: Collapse of morphological species in the wild potatoSolanum brevicaule complex (Solanaceae: sect.Petota). — Amer. J. Bot.85: 92–109.

    Google Scholar 

  • Van Heusden, A. W., Bachmann, K., 1992: Genotype relationships inMicroseris elegans (Asteraceae, Lactuceae) revealed by DNA amplification from arbitrary primers (RAPDs). — Pl. Syst. Evol.179: 221–233.

    Google Scholar 

  • White, P. S., Gilbert, M. L., Nguyen, H. T., Vierling, R. A., 1995: Maximum parsimony accurately reconstructs relationships of eliteSorghum lines. — Crop Sci.35: 1560–1565.

    Google Scholar 

  • Wilkie, S. E., Isaac, P. G., Slater, R. J., 1993: Random amplified DNA (RAPD) markers for genetic analysis inAllium. — Theor. Appl. Genet.86: 497–504.

    Google Scholar 

  • Williams, C. E., St. Clair, D. A., 1993: Phenetic relationships and levels of variability detected by restriction fragment length polymorphism and random amplified polymorphic DNA analysis of cultivated and wild accessions ofLycopersicon esculentum. — Genome36: 619–630.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Vegetable Crops Research Unit, USDA, Agricultural Research Service, Department of Horticulture, University of Wisconsin, 1575 Linden Drive, 53706-1590, Madison, Wisconsin, USA

    Joseph T. Miller & David M. Spooner

Authors
  1. Joseph T. Miller
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. David M. Spooner
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Miller, J.T., Spooner, D.M. Collapse of species boundaries in the wild potatoSolanum brevicaule complex (Solanaceae, S. sect.Petota): Molecular data. Pl Syst Evol 214, 103–130 (1999). https://doi.org/10.1007/BF00985734

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00985734

Key words

Search

Navigation