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Transgenic grain legumes obtained byin planta electroporation-mediated gene transfer

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Abstract

Electroporation-mediated gene transfer into intact plant tissues was demonstrated in pea, cowpea, lentil, and soybean plants. Transient expression of a chimericgus reporter gene was used to monitor the uptake and expression of the introduced DNA in electroporated nodal axillary buds in vivo. The branches that grew out of the nodal meristems were chimeric and expressed the introduced gene up to 20 d after electroporation. Transgenic R1 pea, lentil, and cowpea plants were recovered from seeds originating on these chimeric branches as shown by Southern blot hybridization and GUS expression. Transgenic R2 soybean and lentil plants were also obtained. Segregation ratios in these populations showed a strong bias against transgene presence or expression.

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Authors and Affiliations

  1. Departments of Genetics and Cell Biology, Washington State University, 99164-4234, Pullman, WA

    Gangamma M. Chowrira & Paul F. Lurquin

  2. Departments of Crop and Soil Sciences, Washington State University, 99164-4234, Pullman, WA

    Patrick E. Fuerst

  3. Department of Biotechnology, IIHR, Hessaraghatta, 560089, Bangalore, India

    Vani Akella

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  1. Gangamma M. Chowrira
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  2. Vani Akella
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  3. Patrick E. Fuerst
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  4. Paul F. Lurquin
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Chowrira, G.M., Akella, V., Fuerst, P.E. et al. Transgenic grain legumes obtained byin planta electroporation-mediated gene transfer. Mol Biotechnol 5, 85–96 (1996). https://doi.org/10.1007/BF02789058

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  • DOI: https://doi.org/10.1007/BF02789058

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