Abstract
Fusarium solani pisi recombinant cutinase, immobilized by entrapment in calcium alginate and by covalent binding on porous silica, was used to catalyze the hydrolysis of tricaprylin. The influence of relevant parameters on the catalytic activity such as pH, temperature, and the substrate concentration were studied. Cutinase immobilized by entrapment presented a Michaelis-Menten kinetics for tricaprylin concentrations up to 200 mM. At higher concentrations of substrate, inhibition was observed. For covalent binding immobilization, diffusional limitations were observed at low substrate concentrations and substrate inhibition occurred for concentrations higher than 150 mM. The stability of immobilized cutinase was also evaluated. The enzyme immobilized by entrapment showed a high stability, in contrast to the immobilization on porous silica.
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Gonçalves, A.P.V., Cabral, J.M.S. & Aires-Barros, M.R. Immobilization of a recombinant cutinase by entrapment and by covalent binding. Appl Biochem Biotechnol 60, 217–228 (1996). https://doi.org/10.1007/BF02783585
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DOI: https://doi.org/10.1007/BF02783585