Abstract
In 1964, a method was described for the determination of free fatty acids (FFAs) in vegetable oil. This paper describes an expansion of that work, improving the sensitivity and reproducibility of the method, as well as examination of solubilities of the copper soaps as a function of chain length and unsaturation. Involvement of the micellar structure was reviewed. Finally, a procedure is described that permits very rapid determination of FFA at the 2.0–14.0 µmol (0.5–4.0 mg oleic acid) level, and the results with several oils are given. Particular attention was given to evaluation of solvent systems which would extract the copper complexes.
Similar content being viewed by others
References
Ayers, C.W., Anal. Chim. Acta 15:77 (1956).
Baker, D., Cereal Chem. 38:47 (1961).
Baker, D., JAOCS 41:21 (1964).
Bains, G.S., S.V. Rao, and D.S. Bhatia, Ibid. 41:832 (1964).
Markley, K.S., Editor, “Fatty Acids,” 2nd edition, Interscience Publishers, Inc., New York, NY, 1960, Part 1, p. 634.
Koenig, A.E., J. Am. Chem. Soc. 36:951 (1914).
Iwayama, Y., J. Pharm. Soc. Japan 79:552 (1959).
Duncombe, W.G., Biochem. J. 88:7 (1963).
Author information
Authors and Affiliations
Additional information
Technical Paper No. 4036, Oregon Agricultural Experiment Station.
About this article
Cite this article
Lowry, R.R., Tinsley, I.J. Rapid colorimetric determination of free fatty acids. J Am Oil Chem Soc 53, 470–472 (1976). https://doi.org/10.1007/BF02636814
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02636814