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One-step isolation of plant DNA suitable for PCR amplification

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Abstract

We report a one-step extraction technique for the isolation of plant DNA, DNA suitable for amplification by PCR can be produced from leaf material smaller than 0.3 mm2 in less than 20 min, with no tube changes. The method was tested on several plant specA00AK020ies. The described method was found to extract DNA that could be amplified without any further purification or treatment. The isolated DNA was amplified using a universal chloroplast primer set. The method was validated by comparing size of PCR products generated by the novel method to PCR products generated using standard DNA isolation techniques.

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Abbreviations

RAPD:

random amplification of polymorphic DNA

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Author notes

  1. Adrian Linacre

    Present address: Forensic Science Unit, Department of Pure & Applied Chemistry, University of Strathclyde, 204 George Street, G1 1XW, Glasgow, UK

Authors and Affiliations

  1. Medical Research Council Toxicology Unit, University of Leicester, UK

    Karen Burr

  2. International Forensic Research Institute, Florida International University, 33199, Miami, Florida

    Ross Harper

  3. Forensic Science Unit, Pure and Applied Chemistry, University of Strathclyde, UK

    Adrian Linacre

Authors
  1. Karen Burr
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  2. Ross Harper
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  3. Adrian Linacre
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Correspondence to Adrian Linacre.

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Burr, K., Harper, R. & Linacre, A. One-step isolation of plant DNA suitable for PCR amplification. Plant Mol Biol Rep 19, 367–371 (2001). https://doi.org/10.1007/BF02772835

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  • DOI: https://doi.org/10.1007/BF02772835

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