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A rapid method for high throughput DNA extraction from plant material for PCR amplification

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Abstract

A rapid and reliable method is described for high throughput extraction of DNA from plant material using glass beads in a flat-bottomed microtitre plate. This procedure is quick, inexpensive, and allows up to 96 samples to be processed in parallel. PCR products produced by the recovered DNA are consistently equivalent to those produced through traditional extraction methods.

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Authors and Affiliations

  1. Viticulture and Horticulture, Swiss Federal Research Station for Fruit-Growing, CH-8820, Wädenswil, Switzerland

    Eve Dilworth & Juerg E. Frey

Authors
  1. Eve Dilworth
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  2. Juerg E. Frey
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Correspondence to Juerg E. Frey.

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Dilworth, E., Frey, J.E. A rapid method for high throughput DNA extraction from plant material for PCR amplification. Plant Mol Biol Rep 18, 61–64 (2000). https://doi.org/10.1007/BF02825295

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  • DOI: https://doi.org/10.1007/BF02825295

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