Abstract
An optimized method for total DNA extraction from genetically modified sugar beet plants has been presented. Uses of high-toxic organic compounds and high-cost extraction kits for nucleic acid isolation are excluded. A comparative analysis of polymerase chain reaction products was performed with amplification of DNA obtained by two methods.
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Original Russian Text © A.S. Hussein, A.A. Nalbandyan, T.P. Fedulova, N.N. Bogacheva, 2014, published in Doklady Rossiiskoi Akademii Sel’skokhozyaistvennykh Nauk, 2014, No. 2, pp. 12–13.
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Hussein, A.S., Nalbandyan, A.A., Fedulova, T.P. et al. Efficient and nontoxic DNA isolation method for PCR analysis. Russ. Agricult. Sci. 40, 177–178 (2014). https://doi.org/10.3103/S1068367414030082
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DOI: https://doi.org/10.3103/S1068367414030082