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Agrobacterium-mediated transformation of élite indica and japonica rice cultivars

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Abstract

A rapid, efficient, routine system has been established forAgrobacterium tumefaciens-mediated production of hundreds of fertile transgenic plants from commercially important rice cultivars, including an indica cultivar, Pusa Basmati 1. Calli induced from embryos of mature rice seeds were cocultivated withA. tumefaciens strain LBA4404 carrying the plasmid pTOK233, then exposed to hygromycin selection followed by an efficient regeneration system. Based on the total number of calli co-cultivated, the transformation frequencies of independent transgenic rice plants including cultivars Pusa Basmati 1, E-yi 105, E-wan 5 and Zhong-shu-wan-geng, were 13.5, 13.0, 9.1, and 9.3%, respectively. T1 seeds were harvested within 7–8 mo of initiation of mature embryo cultures. Data from Southern hybridization analysis proved that foreign genes on T-DNA were stably integrated into the rice genome at low copy/site numbers. Mendelian inheritance of the transgenes was confirmed in T1 progeny.

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Author notes

  1. Jian Zhang

    Present address: Plant Biology Division, The S. R. Noble Foundation, P.O. Box 2180, 73402, Ardmore, OK

  2. Rui-ji Xu

    Present address: Department of Biotechnology, Pioneer Hi-Bred International Inc., P. O. Box 1004, 50131, Johnston, IA

Authors and Affiliations

  1. The Norman Borlaug Institute for Plant Science Research, De Montfort University Scraptoft, LE7 9SU, Leicester, UK

    Jian Zhang, Rui-ji Xu, Malcolm C. Elliott & Dong-Fang Chen

Authors
  1. Jian Zhang
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  2. Rui-ji Xu
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  3. Malcolm C. Elliott
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  4. Dong-Fang Chen
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Correspondence to Dong-Fang Chen.

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Zhang, J., Xu, Rj., Elliott, M.C. et al. Agrobacterium-mediated transformation of élite indica and japonica rice cultivars. Mol Biotechnol 8, 223–231 (1997). https://doi.org/10.1007/BF02760776

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  • DOI: https://doi.org/10.1007/BF02760776

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