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Tryptophan decarboxylase activity in transformed roots fromCatharanthus roseus and its relationship to tryptamine, ajmalicine, and catharanthine accumulation during the culture cycle

  • Secondary Metabolism
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Summary

Tryptophan decarboxylase (TDC), the enzyme that catalyzes the decarboxylation of tryptophan to trytamine, was studied in aCatharanthus roseus transformed root culture. Its activity was evaluated through the culture cycle (36 days), along with the variations in the tryptamine pool as well as the accumulation of alkaloids. Ajmalicine and catharanthine contents in the tissues increased coordinately with an increase in TDC-specific activity after 18 days of growth. No dramatic shifts were observed for the total alkaloid and tryptamine profiles.

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Authors and Affiliations

  1. División de Biología Vegetal, Centro de Investigación Científica de Yucatán, 97310, Cordemex, Yucatán, Mexico

    Ignacio Islas, Víctor M. Loyola-Vargas & María de Lourdes Miranda-Ham

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  1. Ignacio Islas
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  2. Víctor M. Loyola-Vargas
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  3. María de Lourdes Miranda-Ham
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Islas, I., Loyola-Vargas, V.M. & de Lourdes Miranda-Ham, M. Tryptophan decarboxylase activity in transformed roots fromCatharanthus roseus and its relationship to tryptamine, ajmalicine, and catharanthine accumulation during the culture cycle. In Vitro Cell Dev Biol - Plant 30, 81–83 (1994). https://doi.org/10.1007/BF02632125

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  • DOI: https://doi.org/10.1007/BF02632125

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