Skip to main content
SpringerLink
Log in

Indole alkaloid accumulation and tryptophan decarboxylase activity in Catharanthus roseus cells cultured in three different media

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Catharanthus roseus cells were cultured in three types of media. These media were: a low sucrose subculture medium and two high sucrose media, each of which differed in their mineral and hormonal contents. The kinetics of tryptophan decarboxylase activity and the accumulations of tryptophan, tryptamine, ajmalicine and serpentine were different in each series but no correlation between maximum enzyme activity and alkaloid contents was observed. Ajmalicine and serpentine productions were unaffected by addition of Trp to the media, whereas addition of secologanin enhanced alkaloid production. The results seem to imply that the terpenoid pathway is the limiting factor in alkaloid production in C. roseus cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bradford MM (1976) Anal. Biochem., 72: 248–254

    Google Scholar 

  • Deus B, Zenk MH (1982) Biotechn. Bioengin., 24: 1965–1974

    Google Scholar 

  • Deus-Neumann B, Zenk MH (1984) Planta med., 50: 427–431

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Exp. Cell. Res., 50: 151–158

    Google Scholar 

  • Gibson RA, Barrett G, Wightman F (1972) J. Exp. Bot., 23: 775–786

    Google Scholar 

  • Kinast G., Tietze LF (1976) Chem. Ber., 109: 3640–3645

    Google Scholar 

  • Knobloch KH, Berlin J (1981) Planta med., 42: 167–172

    Google Scholar 

  • Knobloch KH, Hansen B, Berlin J (1981) Z. Naturforsch., 36c: 40–43

    Google Scholar 

  • Mérillon JM, Rideau M, Chénieux JC (1983) Planta med., 47, 169–176

    Google Scholar 

  • Mérillon JM, Rideau M, Chénieux JC (1984) Planta med., 50, 497–501

    Google Scholar 

  • Sasse F., Buchholz M, Berlin J (1983) Z. Naturforsch., 38: 916–922

    Google Scholar 

  • Zenk HM, El Shagi H, Arens H, Stöckigt J, Weiler EW, Deus B (1977) in Barz W, Reinhard E, Zenk MH (eds), Plant tissue culture and its biotechnological applications, Springer-Verlag, Berlin, 27–43

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Biotechnologie Végétale, Faculté de Pharmacie, Université de Tours, F-37032, Tours, France

    J. M. Mérillon, J. C. Chénieux & M. Rideau

  2. Laboratoire de Physiologie Végétale, Faculté des Sciences, Université de Tours, F-37032, Tours, France

    P. Doireau & A. Guillot

Authors
  1. J. M. Mérillon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Doireau
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Guillot
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. C. Chénieux
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Rideau
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by W. Barz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mérillon, J.M., Doireau, P., Guillot, A. et al. Indole alkaloid accumulation and tryptophan decarboxylase activity in Catharanthus roseus cells cultured in three different media. Plant Cell Reports 5, 23–26 (1986). https://doi.org/10.1007/BF00269710

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00269710

Keywords

Search

Navigation