Abstract
The absorption of equivalent doses of eicosapentaenoic and docosahexaenoic acids was compared in rats when administered as the ethyl ester concentrate, ethyl ester concentrate plus olive oil, free fatty acid or triacylglycerol (menhaden oil). Lymph was collected from a thoracic duct cannula for 24 hr after dosing via an indwelling duodenal catheter. After 24 hr, the absorption of eicosapentaenoic acid was greater for the free fatty acid and menhaden oil than for the ethyl ester form, but docosahexaenoic acid absorption was comparable for all forms. Other rats had greater plasma levels of eicosapentaenoic and docosahexaenoic acids 5 hr after oral gavage dosing with menhaden oil than did rats dosed with the ethyl ester form.
Similar content being viewed by others
Abbreviations
- C:
-
lipid-free emulsion medium
- DHA:
-
docosahexaenoic acid
- EE:
-
ethyl ester concentrate
- EPA:
-
eicosapentaenoic acid
- FFA:
-
free fatty acid
- MO:
-
menhaden oil
- OO:
-
olive oil
References
Hamazaki, T., Urakaze, M., Makuta, M., Ozawa, A., Soda, Y., Tatsumi, H., Yano, A., and Kumagai, A. (1987)Lipis 22, 994–998.
Boustani, S.E., Colette, C., Monnier, L., Descomps, B., Crastes de Paulet, A., and Mendy, F. (1987)Lipids 22, 711–714.
Lawson, L.D., and Hughes, B.G. (1988)Biochem. Biophys. Res. Commun. 152, 328–335.
Lawson, L.D., and Hughes, B.G. (1988)Biochem. Biophys. Res. Commun. 156, 960–963.
Vahouny, G.V., Satchithanandam, S., Chen, I., Tepper, S.A., Kritchevsky, D., Lightfoot, F.G., and Cassidy, M.M. (1988)Am. J. Clin. Nutr. 47, 201–206.
Bollman, J.L., Cain, J.C., and Grindlay, J.H. (1948)J. Lab. Clin. Med. 33, 1349–1352.
Vahouny, G.V., Fawal, I., and Treadwell, C.R. (1957)Am. J. Physiol. 188, 342–346.
Einig, R.G., and Ackman, R.G. (1987)J. Am. Oil Chem. Soc. 64, 499–502.
Folch, J., Lees, M., and Stanley, G.H.S. (1957)J. Biol. Chem. 226, 497–509.
Van Dolah, F.M., and Galloway, S.B. (eds.), National Oceanic and Atmospheric Administration Technical Memorandum NMFS-SEFS-211,Biomedical Test Materials Program: Analytical Methods for Quality Assurance of Fish Oil, National Manine Fisheries Service, Charleston Laboratory, Charleston, SC, November 1988, pp. 6–11.
Nelson, G.J., and Ackman, R.G. (1988)Lipids 23, 1005–1014.
Hamazaki, T., Hirai, A., Terano, T., Sajiki, J., Kondo, S., Fujita, T., Tamura, Y., and Kumagai, A. (1982)Prostaglandins 23, 557–567.
Terano, T., Hirai, A., Hamazaki, T., Kobayashi, S., Fujita, T., Tamura, Y., and Kumagai, A. (1983)Atherosclerosis 46, 321–331.
Yang, L.-Y., Kuksis, A., and Myher, J.J. (1989)Biochem. Cell Biol. 67, 192–204.
von Schacky, C., and Weber, P.C. (1985)J. Clin. Invest. 76, 2446–2450.
Nagakawa, Y., Orimo, H., Harasawa, M., Morita, I., Yashiro, K., and Murota, S. (1983)Atherosclerosis 47, 71–75.
Kobatake, Y., Kuroda, K., Jinnouchi, H., Nishide, E., and Innami, S. (1984)J. Nutr. Sci. Vitaminol. 30, 357–372.
Author information
Authors and Affiliations
About this article
Cite this article
Reicks, M., Hoadley, J., Satchithanandam, S. et al. Recovery of fish oil-derived fatty acids in lymph of thoracic duct-cannulated wistar rats. Lipids 25, 6–10 (1990). https://doi.org/10.1007/BF02562420
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02562420