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Efficient leaching of cellulases produced byTrichoderma harzianum in solid state fermentation

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Biotechnology Techniques

Summary

Recovery of cellulases from solid state cultures ofTrichoderma harzianum was efficiently achieved by hydraulic pressing. Pressing of fermented solids yielded carboxymethyl-cellulase (CMCase) extraction efficiency of 71% and a ratio of leachate to fermented solids of 0.58 (v/w). Addition of water to pressed solids and second pressing improved the efficiency (95%) with simultaneous increase in the ratio to 1.16 (v/w). The overall extraction of filter paper activity was lower (85%) than that of CMCase. This technique is simple and its extraction efficiency is similar to that obtained in multiple-contact counter-current systems. The hydraulic press in its individuality was not used earlier to leach the product from fermented solids.

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Authors and Affiliations

  1. Center Montpellier, Biotechnology Unit, ORSTOM, 911, Avenue Agropolis, BP 5045, 34032, Montpellier, France

    S. Roussos & M. Raimbault

  2. Department of Biotechnology, Autonomous Metropolitan University, Iztapalapa Campus, AP 55-535, CP 09340, Mexico, DF, Mexico

    G. Saucedo-Castañeda

  3. Fermentation Technology and Bioengineering Discipline, Central Food Technological Research Institute, 570 013, Mysore, India

    B. K. Lonsane

Authors
  1. S. Roussos
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  2. M. Raimbault
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  3. G. Saucedo-Castañeda
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  4. B. K. Lonsane
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Roussos, S., Raimbault, M., Saucedo-Castañeda, G. et al. Efficient leaching of cellulases produced byTrichoderma harzianum in solid state fermentation. Biotechnol Tech 6, 429–432 (1992). https://doi.org/10.1007/BF02447483

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  • DOI: https://doi.org/10.1007/BF02447483

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