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Enhanced enzymatic hydrolysis of mild alkali pre-treated rice straw at high-solid loadings using in-house cellulases in a bench scale system

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Abstract

In the present study, scale-up systems for cellulase production and enzymatic hydrolysis of pre-treated rice straw at high-solid loadings were designed, fabricated and tested in the laboratory. Cellulase production was carried out using tray fermentation at 45 °C by Aspergillus terreus in a temperature-controlled humidity chamber. Enzymatic hydrolysis studies were performed in a horizontal rotary drum reactor at 50 °C with 25 % (w/v) solid loading and 9 FPU g−1 substrate enzyme load using in-house as well commercial cellulases. Highly concentrated fermentable sugars up to 20 % were obtained at 40 h with an increased saccharification efficiency of 76 % compared to laboratory findings (69.2 %). These findings demonstrate that we developed a simple and less energy intensive bench scale system for efficient high-solid saccharification. External supplementation of commercial β-glucosidase and hemicellulase ensured better hydrolysis and further increased the saccharification efficiency by 14.5 and 20 %, respectively. An attempt was also made to recover cellulolytic enzymes using ultrafiltration module and nearly 79–84 % of the cellulases and more than 90 % of the sugars were recovered from the saccharification mixture.

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Acknowledgments

The authors are thankful to the Director, Sardar Patel Renewable Energy Research Institute (SPRERI), Vallabh Vidyanagar, Gujarat for providing facilities and resources to carry out this research at SPRERI. The financial support from Department of Biotechnology (DBT), Government of India is highly acknowledged.

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Authors and Affiliations

  1. Sardar Patel Renewable Energy Research Institute, P. Box No. 2, Vallabh Vidyanagar, 388 120, Gujarat, India

    Madhuri Narra, Velmurugan Balasubramanian & Jisha P. James

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  1. Madhuri Narra
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  2. Velmurugan Balasubramanian
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  3. Jisha P. James
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Correspondence to Madhuri Narra.

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Narra, M., Balasubramanian, V. & James, J.P. Enhanced enzymatic hydrolysis of mild alkali pre-treated rice straw at high-solid loadings using in-house cellulases in a bench scale system. Bioprocess Biosyst Eng 39, 993–1003 (2016). https://doi.org/10.1007/s00449-016-1578-9

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  • DOI: https://doi.org/10.1007/s00449-016-1578-9

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