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Solid-liquid extraction of cellulases from fungal solid-state cultivation in a packed bed bioreactor

  • Biotechnology
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Abstract

Cellulases are enzymes required for the production of second-generation ethanol (E2G) via biochemical route. The current paper reports the development of an apparatus for solid-liquid extraction of cellulases from solid-state fermentation (SSF) carried out in a packed bed bioreactor (PBB), operated as batch and as semicontinuous. The case study was the cultivation of Myceliophthora thermophila I-1D3b on sugarcane bagasse (SCB) and wheat bran (WB) (7: 3 w/w). The current work integrates the PBB to the first downstream step for recovering the enzymes produced by SSF. The substrate was inoculated and packed into the modules that composed the PBB. The fermentation occurred at 45 °C and air was supplied with flow rate of 350 L/h. At the end of the cultivations, each module was placed in an extraction column, a dynamic closed system in which distilled water was circulated and made to percolate the cultivated material. Variables tested were volume of water per mass of substrate, water flow rate and time of percolation. Higher contact time (120 min) and higher flow rate (2.4m3/h) allowed us to recover up to 85% of total enzyme activity by percolation. Lower volume (20mL/g) provides higher titer extract. The apparatus showed to be promising for SSF downstream, mainly for semicontinuous operation of PPBs.

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Acknowledgements

The authors are grateful for the financial support of the Säo Paulo Research Foundation (FAPESP) (grant number 2018/009962, 2018/26097-4 and 2018/16689-1), of Coordination for the Improvement of Higher Education Personnel (CAPES — Finance Code 001) and of Brazilian National Council for Scientific and Technological Development (CNPq, grant number 430786/2018-2).

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Authors and Affiliations

  1. Department of Chemical Engineering, Federal University of São Carlos (UFSCar), Rodovia Washington Luiz km 235 (SP 310), 13565-905, São Carlos, SP, Brazil

    Samuel Pratavieira de Oliveira, Natalia Alvarez Rodrigues & Fernanda Perpétua Casciatori

  2. Department of Chemistry and Environmental Sciences, Institute of Biosciences, Letters and Exact Sciences, São Paulo State University (UNESP), Cristóvão Colombo 2265, 15054-000, São José do Rio Preto, SP, Brazil

    Priscila Aparecida Casciatori-Frassatto

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  1. Samuel Pratavieira de Oliveira
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  2. Natalia Alvarez Rodrigues
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  3. Priscila Aparecida Casciatori-Frassatto
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  4. Fernanda Perpétua Casciatori
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Correspondence to Fernanda Perpétua Casciatori.

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de Oliveira, S.P., Alvarez Rodrigues, N., Casciatori-Frassatto, P.A. et al. Solid-liquid extraction of cellulases from fungal solid-state cultivation in a packed bed bioreactor. Korean J. Chem. Eng. 37, 1530–1540 (2020). https://doi.org/10.1007/s11814-020-0579-1

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  • DOI: https://doi.org/10.1007/s11814-020-0579-1

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