Skip to main content
SpringerLink
Log in

Measurement of intracellular sodium and potassium in cultured epithelial cells

  • Published:
Journal of tissue culture methods

Summary

A quantitative method is described for the measurement of intracellular Na+ and K+ using flame photometry. This technique allows the determination of Na+ and K+ from a single sample derived from submilligram quantities of cellular protein. The method is suitable for definition of net ion transport in cultured epithelial cells, and has been established for use with both renal and intestinal cell lines.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

V. References

  1. Fisher, R. S.; Spring, K. R. Intracellular activities during volume regulation byNecturus gallbladder. J. Membr. Biol. 78:187–199; 1984.

    CAS  PubMed  Google Scholar 

  2. Ganz, M. B.; Boyarsky, G.; Sterzel, R. B., et al. Arginine vasopressin enhances pHi regulation in the presence of HCO −3 by stimulating three acid-base transport systems. Nature 337:648–651; 1989.

    Article  CAS  PubMed  Google Scholar 

  3. Harootunian, A. T.; Kao, J. P. Y.; Eckert, B. K., et al. Fluorescence ratio imaging of cytosolic free Na+ in individual fibroblasts and lymphocytes. J. Biol. Chem. 264(32):19458–19467; 1989.

    CAS  PubMed  Google Scholar 

  4. Helmle-Kolb, C.; Montrose, M. H.; Stange, G., et al. Regulation of Na+/H+ exchange in opossum kidney cells by parathyroid hormone, cyclic AMP and phorbol esters. Pfluegers Arch. 415:461–470; 1990.

    Article  CAS  Google Scholar 

  5. Huet, C.; Sahuquillo-Merino, C.; Coudrier, E., et al. Absorptive and mucussecreting subclones isolated from a multipotent intestinal cell line (HT-29) provide new models for cell polarity and terminal differentiation. J. Cell Biol. 105:345–357; 1987.

    Article  CAS  PubMed  Google Scholar 

  6. Kimmich, G. A. Membrane potentials and the mechanism of intestinal Na(+)-dependent sugar transport. J. Membr. Biol. 114(1):1–27; 1990.

    CAS  PubMed  Google Scholar 

  7. Montrose, M. H.; Knoblauch, C.; Murer, H. Separate control of regulatory volume increase and Na+-H+ exchange by cultured renal cells. Am. J. Physiol. 255:C76-C85; 1988.

    CAS  PubMed  Google Scholar 

  8. Montrose, M. H.; Murer, H. Regulation of intracellular pH in LLC-PK1 cells by Na+/H+ exchange. J. Membr. Biol. 93:33–42; 1986.

    CAS  PubMed  Google Scholar 

  9. Schultz, S. G.; Hudson, R. L. How do sodium-absorbing cells do their job and survive? News Physiol. Sci. 1:185–189; 1986.

    CAS  Google Scholar 

  10. Verrey, F.; Kairouz, P.; Schaerer, E., et al. Primary sequence ofXenopus laevis Na+-K+-ATPase and its localization in A6 kidney cells. Am. J. Physiol. 256:F1034-F1043; 1989.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medicine, Johns Hopkins University, 725 N. Wolfe Street, 21205, Baltimore, Maryland

    M. H. Montrose

Authors
  1. M. H. Montrose
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This study was supported by funds from the Myerhoff Digestive Disease Center at the Johns Hopkins University.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Montrose, M.H. Measurement of intracellular sodium and potassium in cultured epithelial cells. Journal of Tissue Culture Methods 13, 211–215 (1991). https://doi.org/10.1007/BF02388129

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02388129

Key words

Search

Navigation