Summary
The inheritance of phaseolin and globulin-2 (G2)/albumin polypeptides was investigated in crosses involving varieties which exhibited the three electrophoretic banding patterns of phaseolin found in French bean. ‘Total’ seed protein extracts of single seeds of the F1 and F2 generations from the crosses: ‘Sanilac’ × ‘Contender’, ‘BBL 240’ × ‘Contender’, and ‘Sanilac’ × ‘BBL 240’ were analyzed by two-dimensional electrophoresis. Segregation of the genes controlling phaseolin and G2/albumin polypeptides, and those controlling a further five groups of seed proteins (A, B, D, E, and F) were observed. No recombinant electrophoretic phenotypes were seen for phaseolin or G2/albumin polypeptides suggesting that the genes controlling each of these groups of polypeptides are closely linked and segregate like single Mendelian genes. The phaseolin genes and G2/albumin genes were not linked to each other. The group of genes controlling phaseolin polypeptides were linked to those controlling group B proteins, and those controlling G2/albumin polypeptides were linked to those controlling group F proteins.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature
Bietz, J.A.; Huebner, J.E.; Sanderson, J.E.; Wall, J.S. (1977): Wheat gliadin homology revealed through N-terminal amino acid sequence analysis. Cereal Chem.54, 1070–1083
Bietz, J.A.; Paulis, J.W.; Wall, J.S. (1979): Zein subunit homology revealed through amino-terminal sequence analysis. Cereal Chem.56, 327–332
Bollini, R.; Crispeels, M.J. (1978): Characterization and subcellular localization of vicilin and phytohaemagglutinin, the two major reserve proteins ofPhaseolus vulgaris L. Planta142, 291–298
Brown, J.W.S.; Kemble, R.J.; Law, C.N.; Flavell, R.B. (1979): Control of endosperm proteins inTriticum aestivum (var. ‘Chinese Spring’) andAegilops umbellulata by homoeologous group 1 chromosome. Genetics93, 189–200
Brown, J.W.S.; Bliss, F.A.; Hall, T.C. (1980): Microheterogeneity of G1 storage protein from French bean with isoelectrofocusing. Plant Physiol.66, 838–840
Brown, J.W.S.; Ma, Y.; Bliss, F.A.; Hall, T.C. (1981a): Genetic variation in the subunits of globulin-1 storage protein of French bean. Theor. Appl. Genet.59, 83–88
Brown, J.W.S.; Flavell, R.B. (1981b): Fractionation of wheat and gliadin glutenin subunits by two-dimensional electrophoresis and the role of group 6 and group 2 chromosomes in gliadin synthesis. Theor. Appl. Genet.59 (1981) 349–359
Brown, J.W.S.; Osborn, T.C.; Bliss, F.A.; Hall, T.C. (1981c): Genetic variation in the subunits of the globulin-2 and albumin seed proteins of French bean. Theor. Appl. Genet.
Brücher, O. (1968): Absence of phytohaemagglutinin in wild and cultivated beans from South America. Proc. Tropical Region. Amer. Soc. Hort. Sci.12, 68–85
Buchbinder, B.U. (1980): Polysomal and messenger RNA directed in vitro synthesis and processing of phaseolin, the G1 seed storage protein ofPhaseolus vulgaris L. Ph.D. Thesis, University of Wisconsin, Madison
Casey, R. (1979): Genetic variability in the structure of theαsubunits of legumin fromPisum — a two-dimensional electrophoresis study. Heredity43, 265–272
Davies, D.R.; Bedford, I. (1979): The ra locus and legumin synthesis in peas. 70th Ann. Rep. John Innes Institute Norwich: John Innes Institute
Gatehouse, J.A.; Croy, R.R.D.; Boulter, D. (1980): Isoelectric-focusing properties and carbohydrate content of pea (Pisum sativum) legumin. Biochem. J.185, 497–503
Hall, T.C.; McLeester, R.C.; Bliss, F.A. (1977): Equal expression of the maternal and paternal alleles for polypeptide subunits of the major storage protein of the beanPhaseolus vulgaris L. Plant Physiol.59, 1122–1124
Hall, T.C.; Ma, Y.; Buchbinder, B.U.; Pyne, J.W.; Sun, S.M.; Bliss, F.A. (1978): Messenger RNA for G1 protein of French bean seeds: cell-free translation and product characterization. Proc. Nat. Acad. Sci. USA75, 3196–3200
Hall, T.C.; Sun, S.M.; Buchbinder, B.U.; Pyne, J.W.; Bliss, F.A.; Kemp, J.D. (1980): Bean seed globulin mRNA: Translation, characterization, and its use as a probe towards genetic engineering of crop plants. In: Genome Organization and Expression in Plants (ed.: Leaver, C.J.), pp. 259–272. New-York, London: Plenum Publ.
Jaffé, W.G.; Brücher, O.; Palozzo, A. (1972): Detection of four types of specific phytohaemagglutinins in different lines of beans. Z. Immunitätsforsch.142, 439–447
Ma, Y.; Bliss, F.A. (1978): Seed proteins of common bean. Crop Sci.17, 431–437
Ma, Y.; Bliss, F.A.; Hall, T.C. (1980): Peptide mapping reveals considerable sequence homology between the three polypeptide subunits of G1 storage protein from French bean seed. Plant Physiol.66, 897–902
Mc.Leester, R.C.; Hall, T.C.; Sun, S.M.; Bliss, F.A. (1973): Comparison of globulin proteins fromPhaseolus vulgaris with those fromVicia faba. Phytochemistry12, 85–93
Mecham, D.K.; Kasarda, D.D.; Qualset, C.O. (1978): Genetic aspects of wheat gliadin proteins. Biochem. Genet.16, 831–853
Miflin, B.J.; Shewry, P.R. (1979): The biology and biochemistry of cereal seed prolamins. In: Seed Protein Improvement in Cereals and Grain Legumes, pp. 137–158. Vienna: Inter. Atomic Energy Agency
Miller, J.B.; Hsu, R.; Heinrikson, R.; Yachnin, S. (1975): Extensive homology between the subunits of the phytohaemagglutinin mitogenic proteins derived fromPhaseolus vulgaris. Proc. Nat. Acad. Sci. USA72, 1388–1391
O'Farrell, P.H. (1975): High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem.250, 4007–4021
Righetti, P.G.; Gianazza, E.; Viotti, A.; Soave, C. (1977): Heterogeneity of storage proteins in maize. Planta136, 115–123
Romero, J.; Sun, S.M.; McLeester, R.C.; Bliss, F.A.; Hall, T.C. (1975): Heritable variation in a polypeptide subunit of the major storage protein of the bean (Phaseolus vulgaris L.) Plant Physiol.56, 776–779
Shewry, P.R.; Faulks, A.J.; Pickering, R.A.; Jones, I.T.; Finch, R.A.; Miflin, B.J. (1980): The genetic analysis of barley storage proteins. Heredity44, 383–389
Soave, C.; Suman, N.; Viotti, A.; Salamini, F.: (1978): Linkage relationships between regulatory and structural gene loci involved in zein synthesis in maize. Theor. Appl. Genet.52, 263–267
Sun, S.M.; Buchbinder, B.U.; Hall, T.C. (1975): Cell-free synthesis of the major storage protein of the bean,Phaseolus vulgaris L. Plant Physiol.56, 780–785
Sun, S.M.; Hall, T.C. (1975): Solubility characteristics of globulins fromPhaseolus vulgaris seeds in regard to their isolation and characterization. J. Agric. Food Chem.23, 184–189
Sun, S.M.; McLeester, R.C.; Bliss, F.A.; Hall, T.C. (1974): Reversible and irreversible dissociation of globulins fromPhaseolus vulgaris seeds. J. Biol. Chem.249, 2118–2121
Thanh, V.H.; Shibasaki, K. (1978): Major proteins of soybean seeds. Subunit structure ofβ-conglycinin. J. Agric. Food Chem.26, 692–695
Valentini, G.; Soave, C.; Ottaviano, E. (1979): Chromosomal location of zein genes inZea mays. Heredity42, 33–40
Viotti, A.; Pogna, N.E.; Balducci, C.; Durante, M. (1980): Chromosomal localisation of zein genes in situ hybridisation inZea mays. Molec. Gen. Genet.178, 35–41
Wrigley, C.W.; Shepherd, K.W. (1973): Electrofocusing of grain proteins from wheat genotypes. Ann. N. Y. Acad. Sci.209, 154–162
Author information
Authors and Affiliations
Additional information
Communicated by H.F. Linskens
Rights and permissions
About this article
Cite this article
Brown, J.W.S., Bliss, F.A. & Hall, T.C. Linkage relationships between genes controlling seed proteins in French bean. Theoret. Appl. Genetics 60, 251–259 (1981). https://doi.org/10.1007/BF02342545
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02342545