Skip to main content
SpringerLink
Log in

Evaluating genetic biodiversity of Parkia biglobosa (Jacq.) R. Br. Ex Don (African locust bean) accessions from Nigeria using seed protein electrophoresis

  • Research Article
  • Published:
Journal of Crop Science and Biotechnology Aims and scope Submit manuscript

Abstract

Thirty-four accessions of African locust bean (Parkia biglobosa) collected from different localities across Nigeria were screened for variability in seed storage proteins. Globulin, albumin, and vicilin fractions were investigated by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and native PAGE. Both methods of analysis revealed a substantial degree of heterogeneity in the protein fractions. However, native PAGE produced fewer protein bands and profiles than SDS-PAGE. Although accessions exhibited significant similarity in their polypeptide profiles, differences in migration rate and staining intensities as well as presence / absence of specific bands accounted for variations obtained in the accessions examined. The absence of 10 kDa albumin subunit was specific to accessions 24, 25, 26, and 29 while the presence of 30.4 kDa vicilin was unique to accessions 5, 6, and 7. One major cluster of globulin band between two polypeptides 42.7 – 50 kDa, and two minor bands 31.4 and 16.2 kDa were observed. Protein fraction profiles were in most cases not similar among the accessions collected from same agroecological zone. This suggests that gene flow between the different zones must have occurred due to movement of germplasm and exchange of genetic material among farmers and markets. Groupings based on cluster analysis and polypeptide patterns agree and confirm the presence of duplicates within the accessions studied.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adesoye AI, Ogunremi CO, Aina OO. 2013. Genetic variation and heritability of seedling traits in African locust bean Parkia biglobosa (Jacq.) R. Br. ex G. Don. Legume Res. 36: 89–97

    Google Scholar 

  • Alabi DA, Akinsulire OR, Sanyaolu MA. 2005. Qualitative determination of chemical and nutritional composition of Parkia biglobosa (Jacq.) Benth. Afri. J. Biotechnol. 4: 812–815

    CAS  Google Scholar 

  • Chaudhary A, Yadav R. 2013. Genetic diversity of maize (Zea mays) hybrids based on protein analysis in different conditions. Ind. J. Agric. Sci. 83: 441–446

    CAS  Google Scholar 

  • Coelho CMM, Bellato CM, Santos JCP, Ortega EMM, Tsai SM. 2007. Effect of phytate and storage conditions on the development of the ‘hard-to-cook’ phenomenon in common beans. J. Sci. Food Agric. 87: 1237–1243

    Article  Google Scholar 

  • De la Rosa L, Gonzalez JM. 2010. The genetic diversity associated with seed proteins in a collection of Spanish underground vetches (Vicia sativa L. subsp. amphicarpa (Dorthes) Asch. Et Graebn.) Genet. Resour. Crop Evol. 57: 565–573

    Article  Google Scholar 

  • Don G. 2007. Seed leaflet. Parkia biglobosa (Jacq). A Tree Species Reference and Selection Guide. 1–4

    Google Scholar 

  • Emre I. 2009. Electrophoretic analysis of some Lathyrus L. species based on seed storage proteins. Genet. Resour. Crop Evol. 56: 31–38

    Article  Google Scholar 

  • Fotso M, Azanza J, Pasquet R, Raymond J. 1994. Molecular heterogeneity of cowpea (Vigna unguiculata Fabaceae) seed storage proteins. Plant Syst. Evol. 191: 39–56

    Article  CAS  Google Scholar 

  • Gbadamosi AE, Faboye OO, Oni OO. 2005. Seed morphological traits, germination and early seedling growth of Parkia biglobosa (Jacq.) R. Br. Ex G. Don from four provenances in Nigeria. Nig. J. For. 35: 129–137

    Google Scholar 

  • Gepts P, Osborne TC, Rashka K, Bliss FA. 1986. Phaseoline protein in wild form variability and landraces of the common bean (Phaseolus vulgaris). Evidence for multiple centre of domestication. Econ. Bot. 40: 451–468

    Article  CAS  Google Scholar 

  • Ghafoor A, Arshad M. 2008. Seed protein profiling of Pisum sativum L. germplasm using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) for investigation of biodiversity. Pak. J. Bot. 40: 2315–2321

    CAS  Google Scholar 

  • Hameed A, Qureshi M, Nawaz M, Iqbal N. 2012. Comparative seed storage protein profiling of Mungbean genotypes. Pak. J. Bot. 44: 1993–1999

    CAS  Google Scholar 

  • Hopkins B. 1983. The taxonomy, reproductive biology and economic potentials of Parkia in Africa and Madagascar. Bot. J. Linnean Soc. 87: 135–167

    Article  Google Scholar 

  • Hussian A, Bushuk W. 1991. Changes in protein electrophoretic pattern during germination of broad beans (Vicia faba L.) with and without hard-to-cook-characteristics. Euphytica 55: 131–139

    Article  Google Scholar 

  • Javaid A, Ghafoor A, Anwar R. 2004. Seed storage protein electrophoresis in groundnut for evaluating genetic diversity. Pak. J. Bot. 36: 25–29

    Google Scholar 

  • Jha SS, Ohri D. 1996. Phylogenetic relationships of Cajanus cajan (L.) Millsp. (pigeonpea) and its wild relatives based on seed protein profiles. Genet. Resour. Crop Evol. 43: 275–281

    Article  Google Scholar 

  • Khan RI, Gatehouse JA, Donald B. 1980. The seed proteins of cowpea (vigna ungiculata L. Walp.. J. Exp. Bot. 31: 1599–1611

    Article  CAS  Google Scholar 

  • Kwon-Ndung EH, Chup CD, Binbol NA, Ismaila A. 2009. Ecological implications of climate change on the genetic diversity and distribution of African locust bean Parkia biglobosa in central Nigeria. Earth Environ. Sci. 6: 37

    Google Scholar 

  • Ladizinsky G, Hymowitz T. 1979. Seed protein electrophoresis in taxonomic and evolutionary studies. Theor. Appl. Genet. 54: 680–685

    Article  Google Scholar 

  • Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685

    Article  CAS  PubMed  Google Scholar 

  • Lassen KM, Rebild A, Hansen H, Brodsgaard CJ, Ericksen EN. 2012. Bats and bees are pollinating Parkia biglobosa in The Gambia. Agroforestry Sys. 85: 465–475

    Article  Google Scholar 

  • Lawal OS, Adebowale KO, Ogunsanwo BM, Sosanwo OA, Bankole SA. 2005. On the functional properties of globulin and albumin protein fractions and flours of African locust bean (Parkia biglobosa). Food Chem. 92: 681–691

    Article  CAS  Google Scholar 

  • Lioi L, Sparvoli F, Sonnante G, Laghetti G, Lupo F, Zaccardelli M. 2011. Characterization of Italian grasspea (Lathyrus sativus L.) germplasm using agronomic traits, biochemical and molecular markers. Genet. Resour. Crop Evol. 58: 425–437

    Article  CAS  Google Scholar 

  • Machuka J. 2001. Heterogeneity in seed globulin and albumin fractions from African yam bean, Sphenostylis stenocarpa (Hoechst, exA. Rich) Harms. Afr. Crop Sci. J. 9: 607–613

    Google Scholar 

  • Mota AC, Fernandes KVS, Sales MP, Flores VMQ, Xavier Filho, J. 2002. Cowpea vicilins: fractionation of urea denatured sub-units and effects on Callosobruchus maculatus F.(Coleoptera: Bruchidae) development. Brazilian Arch. Biol. Technol. 45: 1–5

    Article  CAS  Google Scholar 

  • Muntz K. 1998. Deposition of storage protein. Plant Mol. Biol. 38: 77–99

    Article  CAS  PubMed  Google Scholar 

  • Odeigah PGC, Osanyinpeju AO. 1996. Seed protein electrophoretic characterization of cowpeas, Vigna unguiculata L germplasm from IITA gene bank. Genet. Resour. Crop Evol. 43: 485–491

    Article  Google Scholar 

  • Odeigah PGC, Osanyinpeju AO. 1998. Evaluating genetic biodiversity of Bambara groundnut accessions from Nigeria using SDS polyacrylamide gel electrophoresis. Genet. Resour. Crop Evol. 45: 451–458

    Article  Google Scholar 

  • Odunfa SA. 1986. Dawadawa. In: Legume Based Fermented Foods. CRC Press. Boca Raton, Florida. pp 173–189

    Google Scholar 

  • Okunlola AI, Adebayo RA, Orimogunje AD. 2011. Methods of breaking seed dormancy on germination and early seedling growth of African locust bean (Parkia biglobosa) (Jacq) Benth. J. Hort. For. 3: 1–6

    Google Scholar 

  • Oni PI, Hall JB, Ladipo DO. 1998. The ecology of a key African multipurpose tree species; Parkia biglobosa (Jacq) Benth: the current state of knowledge. Nig. J. Ecol. 1: 59–77

    Google Scholar 

  • Osborne TB. 1924. The vegetable proteins. LongmansGreen, London

    Google Scholar 

  • Osanyinpeju AO, Odeigah PGC. 1998. Variation in seed proteins from mutagen-treated cultivars and selected lines of Vigna unguiculata L. Walp. Plant Breed. 117: 361–365

    Article  CAS  Google Scholar 

  • Protabase 2009. Parkia biglobosa. Accessed March 2014

  • Przybylska J, Zimniak-Przybylska Z, Krajewski P. 2000. Diversity of seed globulins in Lathyrus sativus L. and some related species. Genet. Resour. Crop Evol. 47: 239–246

    Article  Google Scholar 

  • Rao R, Pernolett JC. 1981. Pisum sativum seed globulin as biochemical markers for wrinkled smooth seed character: comparison of genotypes through different protein extraction and electrophoretic procedures. Agronomie 1: 909–916

    Article  Google Scholar 

  • Rao R, Vaglio MD, Paino D, Monti L. 1992. Identification of Vigna species through specific seed storage polypeptides. Euphytica 62: 39–43

    Article  CAS  Google Scholar 

  • Rolf FJ. 1992. NTSYS-pc, numerical taxonomy and multivariate analysis system. Exter New York, NY

    Google Scholar 

  • Rozo C, Bourne MC, Hood LF, Vansoest SJ. 1990. Effect of storage time, relative humidity and temperature on the cookability of whole kidney beans and on cell wall components of cotyledons. Can. Inst. Food Sci. Technol. J. 23: 72–75

    Article  Google Scholar 

  • Sankhon A, Amadou I, Yao W, Wang H, Quian H, Sangare M. 2014. Comparison of physicochemical and functional properties of flour and starch extract in different methods from African locust bean (Parkia biglobosa) seeds. Afr. J. Trad. Comp. Alternative Med. 11: 264–272

    Article  CAS  Google Scholar 

  • Shao M. 2002. Changes in resource allocation in Kandiga, Ghana. Dissertation, Michigan Technological University

    Google Scholar 

  • Shewry PR, Navier JA, Tatham AS. 1995. Seed storage proteins: Structure and biosynthesis. Plant Cell 7: 945–956

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Sina S. 2006. Reproduction et diversite genetique chez Parkia biglobosa (Jacq) G. Don. PhD Thesis. Wageningen University Netherlands.

    Google Scholar 

  • Sina S, Traore SA. 2002. Parkia biglobosa (Jacq.) r. Br. ex G. Don. [internet] PROTA (Plant Resources of Tropical Africa), Wageningen, Netherlands. http:database.prota.org/search.htm.

    Google Scholar 

  • Strelec I, Has-Schon E, Vitale L. 2012. Comparative electrophoretic patterns of albumins/globulins from dry grains and green malts of barley varieties. Poljoprivreda 18: 36–43

    Google Scholar 

  • Teklehaimanot Z. 2004. Exploiting the potential of indigenous agro forestry trees: Parkia biglobosa and Vitellaria paradoxa in Sub-Sahara Africa. Agroforestry Sys. 61: 207–220

    Google Scholar 

  • Thomas JA, Doll H. 1979. Genetics and evolution of seed storage proteins. In: Seed protein improvement in cereal and grain legumes. International Atomic Energy Agency, Vienna, Austria. pp 109–123

    Google Scholar 

  • Thomas JA, Schroeder HE. 1978. Cotyledonary storage protein in Pisum sativum. IIHereditary variation in components of the legumin and vicilin fractions. Austr. J. Plant Physiol. 5: 281–294

    Article  Google Scholar 

  • Uyoh EA, Urua IS, Ntui VO, Okpako EC. 2011. Flow cytometric analysis of nuclear DNA content, mitotic chromosome number and protein separation by SDS-PAGE in three accessions of African locust bean (Parkia biglobosa Benth.). J. Crop. Sci. Biotech. 14: 227–232

    Article  Google Scholar 

  • Wang TL, Domoney C, Hedley CL, Casey R, Grusak MA. 2003. Can we improve the nutritional quality of legume seeds. Plant Physiol. 131: 886–891

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Yunes ANA, Maria TA, Mauricio PS, Rosana AM, Katia VF, Valdirene MG, Jose X. 1998. Legume seed vicilins (7S Storage Proteins) interfere with the development of the cowpea weevil (Callosobruchus maculatus (F). J. Sci. Food Agric. 76: 111–116

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Botany, University of Ibadan, Ibadan, Nigeria

    Adenubi I. Adesoye & Kayode A. Apo

Authors
  1. Adenubi I. Adesoye
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kayode A. Apo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Adenubi I. Adesoye.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Adesoye, A.I., Apo, K.A. Evaluating genetic biodiversity of Parkia biglobosa (Jacq.) R. Br. Ex Don (African locust bean) accessions from Nigeria using seed protein electrophoresis. J. Crop Sci. Biotechnol. 18, 171–180 (2015). https://doi.org/10.1007/s12892-014-0119-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12892-014-0119-9

Keywords

Search

Navigation