Summary
The synthesis of at least 15 zein polypeptides is under the control of regulatory gene loci. One of these, Opaque-2 (chromosome 7, position 16) strongly reduces the zein accumulation without modifying the zein molecular components. The linkage relationship between the regulatory gene 02 and the 5 structural loci (Zp1, Zp2, Zp3, Zp6, Zp12) segregating with sample Mendelian ratios have been studied. Zp1, Zp2, Zp3 are closely linked to each other; moreover this gene cluster is located on chromosome 7 at 5.5 cM from the Opaque-2 locus. The structural loci Zp6 and Zp12 are not linked with each other, with the 02 locus or with Zp1, Zp2, Zp3. From our data it follows that the zein structural genes are located in at least three positions on the maize genome. The scattering in the genome of the genes controlled by the Opaque-2 locus suggests a transacting role for this regulatory element.
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Literature
Alexandrescu, V.; Chihaia, C.; Cosmin, O.: Zein specificity in inbred lines of maize. Rev. Roum. Biochim. 13, 169–172 (1976)
Arst, H.N.; Scazzocchio, C.: Initiator constitutive mutation with an ‘up-promoter’ effect in Aspergillus nidulans. Nature 254, 31–34 (1975)
Arst, H.N.; Scazzocchio, C.: Integrator gene in Aspergillus nidulans. Nature 262, 231–237 (1976)
Burr, B.; Burr, F.A.: Zein synthesis in maize endosperm by polyribosomes attached to protein bodies. Proc. Natl. Acad. Sci. (USA) 73, 515–519 (1976)
Chovnick, A.; Gelbart, W.; McCarron, M.; Osmond, B.; Candido, E.P.M.; Baillie, D.L.: Organization of the rosy locus in Drosophyla melanogaster: evidence for a control element adjacent to the xanthyne deydrogenase structural element. Genetics 84, 233–255 (1976)
Di Fonzo, N.; Fornasari, E.; Salamini, F.; Soave, C: SDS-protein subunits in normal, opaque-2 and floury-2 maize endosperms. Maydica 22, 77–88 (1977)
Douglas, H.C.; Hawtorne, D.C.: Uninducible mutants in the gal i locus of Saccharomyces cerevisiae. J. Bacteriol. 109, 1139–1143 (1972)
Gentinetta, E.; Maggiore, T.; Salamini, F.; Lorenzoni, C.; Pioli, F.; Soave, C.: Protein studies in 46 ‘opaque-2’ strains with modifled endosperm texture. Maydica 20, 145–165 (1975)
Gianazza, E.; Righetti, P.G.; Pioli, F.; Galante, E.; Soave, C.: Size and charge heterogeneity of zein in normal and opaque-2 maize endosperms. Maydica 21, 1–17 (1976)
Gianazza, E.; Viglienghi, V.; Righetti, P.G.; Salamini, F.; Soave, C: Amino acid composition of zein molecular components. Phytochemistry 16, 315–317 (1977)
Immer, F.: Formulae and tables for calculating linkage intensities. Genetics 15, 81–98 (1930)
Larkins, B.A.; Bracker, C.E.; Tsai, C.Y.: Storage protein synthesis in maize. Plant Physiol. 57, 740–745 (1976)
Lee, K.H.; Johns, R.A.; Dalby, A.; Tsai, C.Y.: Genetic regulation of storage protein content in maize endosperm. Biochem. Genet 14, 641–650 (1976)
Lewin, B.: Units of transcription and translation: the relationship between heterogeneous unclear RNA and messenger RNA. Cell 4, 11–20 (1975)
Littlewood, B.S.; Chia, W.; Metzenberg, R.L.: Genetic control of phosphate-metabolizing enzymes in Neurospora crassa: relationships among regulatory mutations. Genetics 79, 419–434 (1975)
Ma, Y.; Nelson, O.E.: Amino acid composition and storage proteins in two new high-lysine mutants in maize. Cereal Chem. 52, 412–419 (1975)
McClintock, B.: Controlling elements and the gene. Cold Spring Harbor Symp. Quant Biol. 21, 197–216 (1956)
McWhirter, K.S.: A floury endosperm, high lysine locus on chromosome 10. Maize Genet. Coop. News Letter 45, 184 (1971)
Mertz, E.T.; Bates, L.S.; Nelson, O.E.: Mutant gene that changes protein composition and increases lysine content of maize endosperm. Science 145, 279–280 (1964)
Misra, P.S.; Mertz, E.T.: Studies on corn proteins. × Polypeptide molecular-weight distribution in Landry — Moreaux fractions of normal and mutant endosperms. Cereal Chem. 53, 705–711 (1976)
Monod, J.; Jacob, F.: General conclusions: teleonomic mechanisms in cellular metabolism, growth and differentiation. Cold Spring Harbor Symp. Quant. Biol. 26, 389–401 (1961)
Mossé, J.: Alcohol-soluble proteins of cereal grains. Fed. Proc. 25, 1663 (1966)
Mossè, J.; Baudet, J.; Landry, J.; Moreaux, T.: Etude comparée sur les proportions et les compositions en acides aminés des fractions protéiques de grains de maïs normaux et mutants. C. Red. Acad. Sci. 263, 788–791 (1966)
Murphy, J.J.; Dalby, A.: Changes in the protein fractions of developing normal and ‘opaque-2’ maize endosperm. Cereal Chem. 48, 336–349 (1971)
Nelson, O.E.; Mertz, E.T.; Bates, L.S.: Second mutant gene affecting the amino acid pattern of maize endosperm proteins. Science 150, 1469–1470 (1965)
Nelson, O.E.: Genetic modification of protein quality in plants. Adv. Agron. 21, 171–194 (1969)
Paigen, K.: The genetics of enzyme realization. In: Enzyme Synthesis and Degradation in Mammalian Systems. (Ed. Rechcigl, M.) pp 1–46. Basel: S. Karger 1971
Righetti, P.G.; Drysdale, J.W.: Isoelectric focusing in polyacrylamide gels. Biochim. Biophys. Acta 236, 17–28 (1974)
Righetti, P.G.; Gianazza, E.; Salamini, F.; Galante, E.; Viotti, A.; Soave, C.: Zein: macromolecular properties, biosynthesis and genetic regulation. In: Electrofocusing and Isotachophoresis. pp. 199–211. Berlin-New York: Gruyter & Co. 1977a
Righetti, P.G.; Gianazza, E.; Viotti, A.; Soave, C.: Heterogeneity of Storage Proteins in Maize. Planta 136, 115–123 (1977b)
Soave, C.; Pioli, F.; Viotti, A.; Salamini, F.; Righetti, P.G.: Synthesis and heterogeneity of endosperm proteins in normal and ‘opaque-2’ maize. Maydica 20, 83–94 (1975)
Soave, C.; Righetti, P.G.; Lorenzoni, C.; Gentinetta, E.; Salamini, F.: Expressivity of the opaque-2 gene at the level of zein molecular components. Maydica 21, 61–75 (1976)
Swank, R.T.; Paigen, K.; Gansschow, R.E.: Genetic control of glucuronidase induction in mice. J. Mol. Biol. 81, 225–243 (1973)
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Communicated by P.L. Pfahler
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Soave, C., Suman, N., Viotti, A. et al. Linkage relationships between regulatory and structural gene loci involved in zein synthesis in maize. Theoret. Appl. Genetics 52, 263–267 (1978). https://doi.org/10.1007/BF00303503
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DOI: https://doi.org/10.1007/BF00303503